LOCUS       sequence-349027-        8934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of wild-type mouse Myc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Mollaoglu G, Guthrie MR, Bohm S, Bragelmann J, Can I, Ballieu PM, 
            Marx A, George J, Heinen C, Chalishazar MD, Cheng H, Ireland AS, 
            Denning KE, Mukhopadhyay A, Vahrenkamp JM, Berrett KC, Mosbruger TL,
            Wang J, Kohan JL, Salama ME, Witt BL, Peifer M, Thomas RK, Gertz J, 
            Johnson JE, Gazdar AF, Wechsler-Reya RJ, Sos ML, Oliver TG
  TITLE     MYC Drives Progression of Small Cell Lung Cancer to a Variant 
            Neuroendocrine Subtype with Vulnerability to Aurora Kinase 
            Inhibition.
  JOURNAL   Cancer Cell. 2017 Feb 13;31(2):270-285. doi: 
            10.1016/j.ccell.2016.12.005. Epub 2017 Jan 12.
  PUBMED    28089889
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.12.005"; journalName: "Cancer Cell"; date: 
            "2017-02-13- 13"; volume: "31"; issue: "2"; pages: "270-285"
COMMENT     Sequence Label: PIG-Myc
FEATURES             Location/Qualifiers
     source          1..8934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             136..735
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(136..155)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     632..652
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    796..1363
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    813..1365
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(957..974)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1184..1203
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1377..1379,1380..1382,1383..2096)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1422..1443)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1683..1702)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2030..2051
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             2198..2712
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    2874..2890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2898..2904,2905..2922,2923..2928))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2943..2964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3081..3098)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3252..3840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3332..3351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4011..4802,4803..4871))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4634..4653
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4872..4976)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5044..5062
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5100..5122)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5222..5241
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5435..5457
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5585..5604
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             5804..6320
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6384..6725
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             6792..7208
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7138..7160
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7176..7192
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        join(8550..8934,1..115)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8585..8608)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
       61 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
      121 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
      181 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
      241 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
      301 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
      361 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
      421 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
      481 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
      541 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
      601 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
      661 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
      721 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
      781 ccccatgcat ccaattccgc cccccccccc taacgttact ggccgaagcc gcttggaata
      841 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
      901 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
      961 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     1021 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     1081 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     1141 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     1201 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     1261 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     1321 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg
     1381 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1441 acgtaaacgg ccacaagttc agcgtgtctg gcgagggcga gggcgatgcc acctacggca
     1501 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1561 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1621 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1681 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1741 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1801 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     1861 tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1921 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1981 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2041 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaatgaa
     2101 ttaattaaga attatcaagc ttatcgatac cgtcgacctg cagccaagct tatcgataaa
     2161 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     2221 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata
     2281 gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat
     2341 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     2401 cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     2461 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     2521 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc
     2581 cgatagactg cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     2641 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     2701 ggggtctttc atgggtaaca gtttcttgaa gttggagaac aacattctga gggtaggagt
     2761 cgaatattaa gtaatcctga ctcaattagc cactgttttg aatccacata ctccaatact
     2821 cctgaaatag ttcattatgg acagcgcaga aagagctggg gagaattgtg aaattgttat
     2881 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2941 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     3001 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3061 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3121 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3181 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3241 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3301 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3361 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3421 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3481 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3541 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3601 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3661 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3721 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3781 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3841 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3901 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3961 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4021 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4081 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4141 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4201 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4261 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4321 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4381 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4441 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4501 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4561 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4621 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4681 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4741 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4801 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4861 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4921 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4981 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     5041 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     5101 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5161 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     5221 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     5281 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     5341 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     5401 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     5461 gcgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     5521 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     5581 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     5641 ccacgatgcg tccggcgtag aggcgattag tccaatttgt taaagacagg atatcagtgg
     5701 tccaggctct agttttgact caacaatatc accagctgaa gcctatagag tacgagccat
     5761 agataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     5821 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact
     5881 gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac
     5941 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     6001 atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     6061 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     6121 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
     6181 agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
     6241 tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc
     6301 cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct gcccagggac
     6361 caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct
     6421 ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact
     6481 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
     6541 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
     6601 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
     6661 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
     6721 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     6781 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     6841 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     6901 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     6961 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     7021 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     7081 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     7141 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     7201 taggcgccgg aattagatct atgcccctca acgtgaactt caccaacagg aactatgacc
     7261 tcgactacga ctccgtacag ccctatttca tctgcgacga ggaagagaat ttctatcacc
     7321 agcaacagca gagcgagctg cagccgcccg cgcccagtga ggatatctgg aagaaattcg
     7381 agctgcttcc caccccgccc ctgtccccga gccgccgctc cgggctctgc tctccatcct
     7441 atgttgcggt cgctacgtcc ttctccccaa gggaagacga tgacggcggc ggtggcaact
     7501 tctccaccgc cgatcagctg gagatgatga ccgagttact tggaggagac atggtgaacc
     7561 agagcttcat ctgcgatcct gacgacgaga ccttcatcaa gaacatcatc atccaggact
     7621 gtatgtggag cggtttctca gccgctgcca agctggtctc ggagaagctg gcctcctacc
     7681 aggctgcgcg caaagacagc accagcctga gccccgcccg cgggcacagc gtctgctcca
     7741 cctccagcct gtacctgcag gacctcaccg ccgccgcgtc cgagtgcatt gacccctcag
     7801 tggtctttcc ctacccgctc aacgacagca gctcgcccaa atcctgtacc tcgtccgatt
     7861 ccacggcctt ctctccttcc tcggactcgc tgctgtcctc cgagtcctcc ccacgggcca
     7921 gccctgagcc cctagtgctg catgaggaga caccgcccac caccagcagc gactctgaag
     7981 aagagcaaga agatgaggaa gaaattgatg tggtgtctgt ggagaagagg caaacccctg
     8041 ccaagaggtc ggagtcgggc tcatctccat cccgaggcca cagcaaacct ccgcacagcc
     8101 cactggtcct caagaggtgc cacgtctcca ctcaccagca caactacgcc gcacccccct
     8161 ccacaaggaa ggactatcca gctgccaaga gggccaagtt ggacagtggc agggtcctga
     8221 agcagatcag caacaaccgc aagtgctcca gccccaggtc ctcagacacg gaggaaaacg
     8281 acaagaggcg gacacacaac gtcttggaac gtcagaggag gaacgagctg aagcgcagct
     8341 tttttgccct gcgtgaccag atccctgaat tggaaaacaa cgaaaaggcc cccaaggtag
     8401 tgatcctcaa aaaagccacc gcctacatcc tgtccattca agcagacgag cacaagctca
     8461 cctctgaaaa ggacttattg aggaaacgac gagaacagtt gaaacacaaa ctcgaacagc
     8521 ttcgaaactc tggtgcatag aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     8581 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     8641 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     8701 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     8761 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     8821 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     8881 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca gggg
//
