LOCUS       sequence-34903-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Kamura T, Sato S, Iwai K, Czyzyk-Krzeska M, Conaway RC, Conaway JW
  TITLE     Activation of HIF1alpha ubiquitination by a reconstituted von 
            Hippel-Lindau (VHL) tumor suppressor complex.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Sep 12. 97(19):10430-5.
  PUBMED    10973499
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Sep 12. 97(19):10430-5."
COMMENT     Sequence Label: pBacPAK8-His-VHL-Flag(117-213)
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             76..98
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             118..135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             442..465
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttcgtacag ttttgtaata aaaaaaccta taaatacgga tccctgcagg cctcgagttc
       61 gaatctagaa ataattttgt ttaactttaa gaaggagata tacatatgcg gggttctcat
      121 catcatcatc atcatggtat ggctagctgg ctcttcagag atgcagggac acacgatggg
      181 cttctggtta accaaactga attatttgtg ccatctctca atgttgacgg acagcctatt
      241 tttgccaata tcacactgcc agtgtatact ctgaaagagc gatgcctcca ggttgtccgg
      301 agcctagtca agcctgagaa ttacaggaga ctggacatcg tcaggtcgct ctacgaagat
      361 ctggaagacc acccaaatgt gcagaaagac ctggagcggc tgacacagga gcgcattgca
      421 catcaacgga tgggggatcc agactacaag gacgatgatg acaagtgaat tcccgggcgg
      481 ccgcttaatt aattgatccg ggttattagt acatttatta agcgctagat tctgtgcgtt
      541 gttgatttac agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag
      601 ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata
      661 ttaaatcctc aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt
      721 ccgaaccgat ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat
      781 cttgtagcag caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt
      841 cgacgtcgtt caaaatatta tgcgcttttg tatttctttc atcactg
//
