LOCUS       sequence-349030-        5926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-mediated expression of histone fused GCaMP8m under the synapsin 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5926)
  TITLE     Looger Lab GCaMP8 plasmids
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-Syn-H2B-jGCaMP8m-WPRE
FEATURES             Location/Qualifiers
     source          1..5926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(117..705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(197..216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(876..1667,1668..1736))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1499..1518
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1737..1841)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1867..2322)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2004..2025)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2216..2235
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2394..2523
                     /label=AAV2 ITR
     promoter        2605..3052
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3086..3463
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3479..3496
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     3778..3799
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3905..3926)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4166..4185)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    4757..5345
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4810..4830)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5228..5239)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5348..5364)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5349..5365)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5394..5515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5405..5424)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5459..5478
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5677..5806
                     /label=AAV2 ITR
ORIGIN
        1 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
       61 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      121 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      181 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      241 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      301 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      361 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      421 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      481 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      541 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      601 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      661 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      721 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      781 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      841 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      901 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      961 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1021 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1081 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1141 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1201 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1261 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1321 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1381 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1441 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1501 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1561 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1621 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1681 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1741 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1801 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1861 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     1921 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     1981 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2041 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2101 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2161 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     2221 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     2281 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     2341 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctgcgcg
     2401 ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc
     2461 cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt
     2521 ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc tctaggaaga
     2581 tctctgcaga gggccctgcg tatgagtgca agtgggtttt aggaccagga tgaggcgggg
     2641 tgggggtgcc tacctgacga ccgaccccga cccactggac aagcacccaa cccccattcc
     2701 ccaaattgcg catcccctat cagagagggg gaggggaaac aggatgcggc gaggcgcgtg
     2761 cgcactgcca gcttcagcac cgcggacagt gccttcgccc ccgcctggcg gcgcgcgcca
     2821 ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc aaactcccct
     2881 tcccggccac cttggtcgcg tccgcgccgc cgccggccca gccggaccgc accacgcgag
     2941 gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg actcagcgct
     3001 gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc agtcgaattc
     3061 aagctgctag caaggatccg ccaccatgcc agagccagcg aagtctgctc ccgccccgaa
     3121 aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg
     3181 cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga
     3241 caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg acattttcga
     3301 gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc
     3361 cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt
     3421 gtccgagggt actaaggcca tcaccaagta caccagcgct aaggacccac cggtcatgca
     3481 tcatcaccat catcacacgc gtcgcaagaa gaccttcaag gaggtggcca ccgccgtgaa
     3541 gatcatcggc cgcctgatgg gcctgaagat caacgtctat atcaaggccg acaagcagaa
     3601 gaacggcatc aaggcgaact tccacatccg ccacaacatc gaggacggcg gcgtgcagct
     3661 cgcctaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3721 ccactacctg agcgtggagt ccaaactttc gaaagacccc aacgagaagc gcgatcacat
     3781 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3841 gggcggtacc ggagggagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3901 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     3961 tgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4021 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4081 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacat
     4141 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4201 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4261 cggcaacatc ctggggcaca agctggagta caacctgccg gaccaactga ctgaagagca
     4321 gatcgcagaa tacaaagagg ctttctccct atttgacaag gacggggatg ggacaataac
     4381 aaccaaggag ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct
     4441 gcaggacatg atcaatgaag tagatgccga cggtgacggc acaatcgact tccctgagtt
     4501 cctgacaatg atggcaagaa aaatgaaata cagggacacg gaagaagaaa ttagagaagc
     4561 gttcggtgtg tttgataagg atggcaatgg ctacatcagt gcagcagagc ttcgccacgt
     4621 gatgacaaac cttggagaga agttaacaga tgaagaggtt gatgaaatga tcagggaagc
     4681 agacatcgat ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa
     4741 gtaaaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4801 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4861 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4921 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4981 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5041 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5101 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     5161 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5221 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5281 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5341 cccgcatcga taccgtcgac ctcgacccgg gcggccgctt cgagcagaca tgataagata
     5401 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     5461 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     5521 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
     5581 caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag catggctacg
     5641 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg
     5701 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     5761 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc attaatgaat
     5821 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5881 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagct
//
