LOCUS       sequence-349044-        5907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  jGCaMP8f expression in GABA-ergic interneurons under the control of 
            mDlx enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5907)
  TITLE     Looger Lab GCaMP8 plasmids
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-mDlx-jGCaMP8f-WPRE
FEATURES             Location/Qualifiers
     source          1..5907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(27..615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(107..126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(786..1577,1578..1646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1409..1428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1647..1751)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1777..2232)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1914..1935)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2126..2145
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2304..2433
                     /label=AAV2 ITR
     intron          3184..3316
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3299..3318
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3370..3387
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     3669..3690
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3796..3817)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4057..4076)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    4648..5236
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4701..4721)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5119..5130)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5239..5255)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5240..5256)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5285..5406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5296..5315)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5350..5369
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5568..5697
                     /label=AAV2 ITR
ORIGIN
        1 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
       61 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      121 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      181 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      241 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      301 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      361 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      421 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      481 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      541 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      601 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      661 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      721 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      781 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
      841 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
      901 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
      961 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1021 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1081 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1141 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1201 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1261 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1321 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1381 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1441 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1501 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1561 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1621 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1681 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     1741 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     1801 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1861 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     1921 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     1981 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     2041 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2101 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2161 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     2221 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2281 gggcctcttc gctattacgc cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa
     2341 agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag
     2401 agggagtggc caactccatc actaggggtt ccttgtagtt aatgattaac ccgccatgct
     2461 acttatctac gtagccatgc tctaggaaga tcttatacac tcacagtggt ttggcatata
     2521 tttggtgaaa ttttttaagg aaaaattagt gttggtttcg atatatggta gctttttctc
     2581 taacataatt tgaataattc agcaaagccc tactaccagc tgtacttctg cagcctcttc
     2641 cattctttcc agcattataa ttttggttaa ttttcaattt taggtcctac gtctctgcaa
     2701 tttgtgtatg aataacagaa taatttccct cttttgtttc gcctttcctg ttcctgaatc
     2761 taaataaaga tggcttttta gtattaaaag tggaagaaaa ttacaggtaa ttatctttga
     2821 cggtaaaaac gctgtaatca gcgggctaca tgaaaaatta ctctaattat ggctgcattt
     2881 aagagaatgg aaaaaaacct tcttgtggat aaaaacctta aattgtcccc aatgtctgct
     2941 tcaaattgga tggcactgca gctggaggct ttgttcagaa ttgatcctgg ggagctacga
     3001 acccaaagtt tcacagtagg aagcttgata tcgaattcct gcagcccggg ctgggcataa
     3061 aagtcagggc agagccatct attgcttaca tttgcttcta gcctgcaggt cgaggagcgc
     3121 agccttccag aagcagagcg cggcgcctta agctgcagaa gttggtcgtg aggcactggg
     3181 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     3241 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     3301 gcctttctct ccacaggtgt ccactcccag ttcaattaca gcgctagcaa ggatccaccc
     3361 gccaccatgc atcatcacca tcatcacacg cgtcgcaaga agaccttcaa ggaggtggcc
     3421 accgccgtga agatcatcgc ccgcctgatg ggcctgaaga tcaacgtcta tatcaaggcc
     3481 gacaagcaga agaacggcat caaggcgaac ttccacatcc gccacaacat cgaggacggc
     3541 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3601 ctgcccgaca accactacct gagcgtggag tccaaacttt cgaaagaccc caacgagaag
     3661 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3721 gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc
     3781 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     3841 tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     3901 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     3961 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     4021 gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4081 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4141 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacctgcc ggaccaactg
     4201 actgaagagc agatcgcaga atttaaagag gctttctccc tatttgacaa ggacggggat
     4261 gggacaataa caaccaagga gctggggacg gtgatgcggt ctctcggact gaaccccaca
     4321 gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac
     4381 ttccctgagt tcctgacaat gatggcaaga aaaatgaaat acagggacac ggaagaagaa
     4441 attagagaag cgttcggtgt gtttgataag gatggcaatg gctacatcag tgcagcagag
     4501 cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt tgatgaaatg
     4561 atcagggaag cagacatcga tggggatggt caggtaaact acgaagagtt tgtacaaatg
     4621 atgacagcga agtaaaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     4681 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4741 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4801 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4861 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4921 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4981 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     5041 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5101 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5161 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     5221 tgggccgcct ccccgcatcg ataccgtcga cctcgacccg ggcggccgct tcgagcagac
     5281 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     5341 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     5401 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     5461 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga
     5521 gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt
     5581 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     5641 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     5701 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5761 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5821 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5881 gcaaaaggcc agcaaaaggc caggaac
//
