LOCUS       sequence-349046-        5907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  jGCaMP8s expression in GABA-ergic interneurons under the control of 
            mDlx enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5907)
  TITLE     Looger Lab GCaMP8 plasmids
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-mDlx-jGCaMP8s-WPRE
FEATURES             Location/Qualifiers
     source          1..5907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1985..2440)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2122..2143)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2334..2353
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2512..2641
                     /label=AAV2 ITR
     intron          3392..3524
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3507..3526
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3578..3595
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     3877..3898
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4004..4025)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4265..4284)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    4856..5444
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4909..4929)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5327..5338)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5447..5463)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5448..5464)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5493..5614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5504..5523)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5558..5577
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5776..5905
                     /label=AAV2 ITR
ORIGIN
        1 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
       61 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2041 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     2101 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     2161 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     2221 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     2281 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     2341 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     2401 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     2461 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctgcgcgct
     2521 cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg
     2581 gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc
     2641 ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc taggaagatc
     2701 ttatacactc acagtggttt ggcatatatt tggtgaaatt ttttaaggaa aaattagtgt
     2761 tggtttcgat atatggtagc tttttctcta acataatttg aataattcag caaagcccta
     2821 ctaccagctg tacttctgca gcctcttcca ttctttccag cattataatt ttggttaatt
     2881 ttcaatttta ggtcctacgt ctctgcaatt tgtgtatgaa taacagaata atttccctct
     2941 tttgtttcgc ctttcctgtt cctgaatcta aataaagatg gctttttagt attaaaagtg
     3001 gaagaaaatt acaggtaatt atctttgacg gtaaaaacgc tgtaatcagc gggctacatg
     3061 aaaaattact ctaattatgg ctgcatttaa gagaatggaa aaaaaccttc ttgtggataa
     3121 aaaccttaaa ttgtccccaa tgtctgcttc aaattggatg gcactgcagc tggaggcttt
     3181 gttcagaatt gatcctgggg agctacgaac ccaaagtttc acagtaggaa gcttgatatc
     3241 gaattcctgc agcccgggct gggcataaaa gtcagggcag agccatctat tgcttacatt
     3301 tgcttctagc ctgcaggtcg aggagcgcag ccttccagaa gcagagcgcg gcgccttaag
     3361 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
     3421 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
     3481 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt
     3541 caattacagc gctagcaagg atccacccgc caccatgcat catcaccatc atcacacgcg
     3601 tcgcaagaag accttcaagg aggtggccac cgccgtgaag atcatcgcca tgctgatggg
     3661 cctgaagatc aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt
     3721 ccacatccgc cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa
     3781 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtggagtc
     3841 caaactttcg aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3901 cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat
     3961 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     4021 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg
     4081 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     4141 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     4201 gcacgacttc ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt
     4261 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     4321 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     4381 gctggagtac aacctgccgg accaactgac tgaagagcag atcgcagaat acaaagaggc
     4441 tttctcccta tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt
     4501 gatgcggtct ctcggacaca accccacaga agcagagctg caggacatga tcaatgaagt
     4561 agatgccgac ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa
     4621 aatgaaatac agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga
     4681 tggcaatggc tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa
     4741 gttaacagat gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca
     4801 ggtaaactac gaagagtttg tacaaatgat gacagcgaag taaaagctta tcgataatca
     4861 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4921 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4981 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5041 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5101 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5161 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5221 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5281 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5341 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5401 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     5461 tcgacccggg cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     5521 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     5581 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     5641 gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     5701 tggtaaaatc gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt
     5761 taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     5821 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     5881 cctcagtgag cgagcgagcg cgcagct
//
