LOCUS       sequence-349051-        5907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  jGCaMP8m expression in GABA-ergic interneurons under the control of 
            mDlx enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5907)
  TITLE     Looger Lab GCaMP8 plasmids
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-mDlx-jGCaMP8m-WPRE
FEATURES             Location/Qualifiers
     source          1..5907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(350..938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(430..449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1109..1900,1901..1969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1732..1751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1970..2074)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2100..2555)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2237..2258)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2449..2468
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2627..2756
                     /label=AAV2 ITR
     intron          3507..3639
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3622..3641
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3693..3710
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     3992..4013
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4119..4140)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4380..4399)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    4971..5559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5024..5044)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5442..5453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5562..5578)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5563..5579)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5608..5729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5619..5638)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5673..5692
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   join(5891..5907,1..113)
                     /label=AAV2 ITR
ORIGIN
        1 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
       61 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcatt
      121 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      181 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      241 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      301 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      361 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      421 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      481 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      541 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      601 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      661 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      721 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      781 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      841 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      901 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      961 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1021 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1081 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1141 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1201 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1261 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1321 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1381 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     1441 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1501 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1561 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1621 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1681 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1741 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1801 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1861 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1921 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1981 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2041 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     2101 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     2161 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     2221 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     2281 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     2341 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     2401 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     2461 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     2521 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa
     2581 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgc gcgctcgctc
     2641 gctcactgag gccgcccggg caaagcccgg gcgtcgggcg acctttggtc gcccggcctc
     2701 agtgagcgag cgagcgcgca gagagggagt ggccaactcc atcactaggg gttccttgta
     2761 gttaatgatt aacccgccat gctacttatc tacgtagcca tgctctagga agatcttata
     2821 cactcacagt ggtttggcat atatttggtg aaatttttta aggaaaaatt agtgttggtt
     2881 tcgatatatg gtagcttttt ctctaacata atttgaataa ttcagcaaag ccctactacc
     2941 agctgtactt ctgcagcctc ttccattctt tccagcatta taattttggt taattttcaa
     3001 ttttaggtcc tacgtctctg caatttgtgt atgaataaca gaataatttc cctcttttgt
     3061 ttcgcctttc ctgttcctga atctaaataa agatggcttt ttagtattaa aagtggaaga
     3121 aaattacagg taattatctt tgacggtaaa aacgctgtaa tcagcgggct acatgaaaaa
     3181 ttactctaat tatggctgca tttaagagaa tggaaaaaaa ccttcttgtg gataaaaacc
     3241 ttaaattgtc cccaatgtct gcttcaaatt ggatggcact gcagctggag gctttgttca
     3301 gaattgatcc tggggagcta cgaacccaaa gtttcacagt aggaagcttg atatcgaatt
     3361 cctgcagccc gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt
     3421 ctagcctgca ggtcgaggag cgcagccttc cagaagcaga gcgcggcgcc ttaagctgca
     3481 gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag
     3541 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
     3601 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt
     3661 acagcgctag caaggatcca cccgccacca tgcatcatca ccatcatcac acgcgtcgca
     3721 agaagacctt caaggaggtg gccaccgccg tgaagatcat cggccgcctg atgggcctga
     3781 agatcaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttccaca
     3841 tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc
     3901 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg gagtccaaac
     3961 tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     4021 ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga
     4081 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4141 taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc tacggcaagc
     4201 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4261 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4321 acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg
     4381 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4441 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4501 agtacaacct gccggaccaa ctgactgaag agcagatcgc agaatacaaa gaggctttct
     4561 ccctatttga caaggacggg gatgggacaa taacaaccaa ggagctgggg acggtgatgc
     4621 ggtctctggg gcagaacccc acagaagcag agctgcagga catgatcaat gaagtagatg
     4681 ccgacggtga cggcacaatc gacttccctg agttcctgac aatgatggca agaaaaatga
     4741 aatacaggga cacggaagaa gaaattagag aagcgttcgg tgtgtttgat aaggatggca
     4801 atggctacat cagtgcagca gagcttcgcc acgtgatgac aaaccttgga gagaagttaa
     4861 cagatgaaga ggttgatgaa atgatcaggg aagcagacat cgatggggat ggtcaggtaa
     4921 actacgaaga gtttgtacaa atgatgacag cgaagtaaaa gcttatcgat aatcaacctc
     4981 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5041 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5101 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5161 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5221 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5281 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5341 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     5401 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5461 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5521 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgac
     5581 ccgggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     5641 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     5701 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     5761 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     5821 aaatcgataa ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc
     5881 attaactaca aggaacccct agtgatg
//
