LOCUS       sequence-349059-       11007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector to introduce a Fucci cell cyle reporter cassette 
            mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using 
            puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur 
            N, Dekker J, Mirny LA, Bruneau BG
  TITLE     Targeted Degradation of CTCF Decouples Local Insulation of 
            Chromosome Domains from Genomic Compartmentalization.
  JOURNAL   Cell. 2017 May 18;169(5):930-944.e22. doi: 
            10.1016/j.cell.2017.05.004.
  PUBMED    28525758
REFERENCE   2  (bases 1 to 11007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.05"; journalName: "Cell"; date: "2017-05-18- 
            18"; volume: "169"; issue: "5"; pages: "930-944.e22"
COMMENT     Sequence Label: pEN435 -
            pCAGGS-TagBFP-hGeminin-2A-mCherry-hCd...
FEATURES             Location/Qualifiers
     source          1..11007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1351..1486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    1591..1612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1627..1632,1633..1650,1651..1657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1665..1681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1689..1705
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2048..2067
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2066..2121
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2167..2186
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2277..2549)
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             complement(2580..3287)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2855..2874)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3124..3142
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(3294..3347)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3363..3692)
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     CDS             complement(3729..4427)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4481..4500)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          4508..5524
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(4562..4584)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        5802..6105
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    6169..6202
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(6301..6324)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6674..6693
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        6753..6800
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6819..7418
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6819..6838)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7315..7335
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7450..7467)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7456..7680
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7735..7768
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8786..8802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8810..8826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8834..8840,8841..8858,8859..8864))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8879..8900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9017..9034)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9188..9776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9268..9287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9947..10738,10739..10807))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10570..10589
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10808..10912)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10980..10998
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtattta aatactgccc ctaggagatg
      421 gatactagca tttgaaactg aggctcatat gtgttatcac tcaaagtgag gtcagactgc
      481 taggaagtta gggaagtggg atttgagcca ctgaaggtcg gaatctgaaa tctggcgagg
      541 tggtaggaag aggtaggaag gtacccaaca gcttcgaaca ggaggaactc ccagatcctt
      601 acaccaaaaa ccacttaggg ttttttgttt tgtttttgtt ttttaaattt cttttctttt
      661 cttccatggg caaagaaaat gactcaggaa gactcagtgg caaccctaga ctcagggcct
      721 cggaagaggc ccacctctgc atcatcctct ccggagcccc ctgagttcag cactttccgg
      781 gcctgccagt gaggctgacg aatgaggacc actttatcca gttccttccc tcccactgcc
      841 agaggctgca catctgtcca gagacttggc agtggaggta gggtgggggt gggaatcaag
      901 ccatagcttt cttagggaag cactggccaa aggaagggga ctcctagagt tgtaaccttc
      961 ctcacagaag acaagaaaat gagttggggt atcagcctca ggctagacag agagccagaa
     1021 cctcttcaca gattcccaga tcaccggaga agtcactatt gaatccggac acccagtcag
     1081 gcacaagagg tctacattct ctaactcctt tccagtgctt ccccaacggt cacttacttc
     1141 cagactgcgt gttttatttt taggagagat gtgtatattt tttgttgctg ttgttgtttc
     1201 tagatagggt ctcactgtgt agccctggct tttctggaac tcactgtgta gagcaagcca
     1261 gcctcaaact catagatcca cctgcctctg cctccagatc gctagaatta aagttactgc
     1321 cataacacct aacatttaaa tcctaaccta ggcctccaaa aaagcctcct cactacttct
     1381 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     1441 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     1501 gcgggactat ggttgctgac taattgagat gcggatccgc tggcacgaca ggtttcccga
     1561 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     1621 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     1681 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgggctgc aggtcgaggg
     1741 atctccataa gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc
     1801 tggctagtaa aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac
     1861 aaaatataaa aaaaatctaa cctcaagtca aggcttttct atggaataag gaatggacag
     1921 cagggggctg tttcatatac tgatgacctc tttatagcca acctttgttc atggcagcca
     1981 gcatatgggc atatgttgcc aaactctaaa ccaaatactc attctgatgt tttaaatgat
     2041 ttgccctccc atatgtcctt ccgagtgaga gacacaaaaa attccaacac actattgcaa
     2101 tgaaaataaa tttcctttat tagccagaag tcagatgctc aaggggcttc atgatgtccc
     2161 cataattttt ggcagaggga aaaagatctc agtggtattt gtgagccagg gcattggcca
     2221 caccagccac caccttctga taggcagcct gcacctgagg ccgcttaccc gggttagatg
     2281 gtgtcctggt cctgcgcgga tgtcaggtgg ggcggctgac ctgctgccgg ggtggatttc
     2341 tttatcttct ggcccggaga agggcaggct gggatgtctg gggcctcggg ggtactgggg
     2401 ctggaaacct cgtccaccga cagccgcagt ctcctgcggg ccggtggcct ggcctggtcg
     2461 cgtccggggg cggcgggcgg gcgggcgcgc ttgcggctgc cactggtagc ggaggccggg
     2521 gcgcggagtg cgggcctggc ggggctgggc tcgagcggcc gccagtgtga tggatatccc
     2581 ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc
     2641 acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg
     2701 ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg
     2761 ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg
     2821 tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg
     2881 gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag
     2941 gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg
     3001 aagccctcgg ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag
     3061 gccttggagc cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca
     3121 cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg
     3181 ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg
     3241 aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatatg catggggccc
     3301 gggttttcct cgacgtcacc acatgtaaga agactccccc gtccctcgcc gcttccctta
     3361 agcagcgcct ttctccgttt ttctgccact tccttccaat actgagagga tggattttct
     3421 ttaatcataa gatcaaatga ctcctgggtg actcctccaa gatttttatt ttcactagat
     3481 tctgggacaa taaccccagg gctggaagtt gtagatgtta agtggtcatt ccgatgtttc
     3541 cttttggaca agcctgcgga cagctcattt tctcttccaa caagagatcc agatgcagaa
     3601 ggctgaatca tcttcagagt tcttcttggg acagaactat tctttatatt ctctttgatt
     3661 tcttcttgtt tctgcttcat actgggattc atctcgagcg gccgccagtg tgatggatat
     3721 cccctaggat taagcttgtg ccccagtttg ctagggaggt cgcagtatct ggccactgcc
     3781 acctcgtgct gctcgacgta ggtctcgttg ttggcctcct tgattctttc cagtctgtag
     3841 tccacatagt agacgccagg catcttgagg ttcttagcgg gtttcttgga tctatatgtg
     3901 gtcttgatgt ttgcgatcag atggctcccg cccacgagct tcagggccat gtcgtttctg
     3961 ccttccaggc cgccgtcagc ggggtacagc gtctcggtga aggcctccca gccgagtgtt
     4021 ttcttctgca tcacagggcc gttggatgtg aagttcaccc ctctgatctt gacgttgtag
     4081 atgaggcagc cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc cccgtcttcg
     4141 tatgtggtga ctctctccca tgtgaagccc tcagggaagg actgcttgaa gaagtcgggg
     4201 atgccctggg tgtggttgat gaaggtcttg ctgccgtaga ggaagctagt agccaggatg
     4261 tcgaaggcga aggggagagg gccgccctcg accaccttga ttctcatggt ctgggtgccc
     4321 tcgtagggct tgccttcgcc ctcggatgtg cacttgaagt gatggttgtc cacggtgccc
     4381 tccatgtaca gcttcatgtg catgttctcc ttaatcagct cgctcatggt ggcgacgcgt
     4441 gagaattctc gaggaattct ttgccaaaat gatgagacag cacaataacc agcacgttgc
     4501 ccaggagctg taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagagccgc
     4561 cggtcacacg ccagaagccg aaccccgccc tgccccgtcc cccccgaagg cagccgtccc
     4621 cccgcggaca gccccgaggc tggagaggga gaaggggacg gcggcgcggc gacgcacgaa
     4681 ggccctcccc gcccatttcc ttcctgccgg cgccgcaccg cttcgccccg cgcccgctag
     4741 agggggtgcg gcggcgcctc ccagatttcg gctccgcaca gatttgggac aaaggaagtc
     4801 cctgcgccct ctcgcacgat taccataaaa ggcaatggct gcggctcgcc gcgcctcgac
     4861 agccgccggc gctccggggg ccgccgcgcc cctcccccga gcccyccccg gcccgaggcg
     4921 gccccgcccc gcccggcacc cccacctgcc gccacccccc gcccggcacg gcgagccccg
     4981 cgccacgccc cgtacggagc cccgcacccg aagccgggcc gtgctcagca actcggggag
     5041 gggggtgcag ggggggttgc agcccgaccg acgcgcccac accccctgct caccccccca
     5101 cgcacacacc ccgcacgcag cctttgttcc cctcgcagcc ccccccgcac cgcggggcac
     5161 cgcccccggc cgcgctcccc tcgcgcacac tgcggagcgc acaaagcccc gcgccgcgcc
     5221 cgcagcgctc acagccgccg ggcagcgcgg agccgcacgc ggcgctcccc acgcacacac
     5281 acacgcacgc accccccgag ccgctccccc cgcacaaagg gccctcccgg agcccctcaa
     5341 ggctttcacg cagccacaga aaagaaacaa gccgtcatta aaccaagcgc taattacagc
     5401 ccggaggaga agggccgtcc cgcccgctca cctgtgggag taacgcggtc agtcagagcc
     5461 ggggcgggcg gcgcgaggcg gcggcggagc ggggcacggg gcgaaggcag cgcgcagcga
     5521 ctcccgcccg ccgcgcgctt cgctttttat agggccgccg ccgccgccgc ctcgccataa
     5581 aaggaaactt tcggagcgcg ccgctctgat tggctgccgc cgcmccycyc csccycsccc
     5641 csccccsccc cccscccccc cccccccccc cyggcgcgcs cccccccccc ccccgccccc
     5701 atcgctgcac aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg
     5761 gagatgggga gagtgaagca gaacgtgggg ctcacctcga ccatggtaat agcgatgact
     5821 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     5881 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     5941 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     6001 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     6061 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     6121 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcga agttcctatt
     6181 ctctagaaag tataggaact tcaggtctga agaggagttt acgtccagcc aagctagctt
     6241 ggctgcaggt cgtcgaaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     6301 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     6361 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     6421 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     6481 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     6541 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     6601 ttctgggctc agaggctggg aaggggtggg tccgggggcg gggcgggcgc ccgaaggtcc
     6661 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     6721 tcctcatctc cgggcctttc gacctgcagc ctgttgacaa ttaatcatcg gcatagtata
     6781 tcggcatagt ataatacgac aaggtgagga actaaaccat gaccgagtac aagcccacgg
     6841 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     6901 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     6961 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     7021 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     7081 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     7141 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     7201 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     7261 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     7321 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     7381 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagg ggatcaattc tctagagctc
     7441 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     7501 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7561 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7621 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     7681 cttctgaggc ggaaagaacc agctggggct cgactagagc ttgcggaacc cttcgaagtt
     7741 cctattctct agaaagtata ggaacttcgg cgcgccattt aaattttggg atgttttctg
     7801 tttgttttga gatagggttt ttcagtgtag tcctggctgt cctagaactc actctgtaga
     7861 ccaggctggc ctggaactca aggactgcca gcctctgcct cctgagcatc gggattaaag
     7921 gtgtgtgcca ccactgccca gcagagtgcg tttttactct tcactttgtg ggtcctggct
     7981 gcctgagatt gtgtgcttgc accattagat gggagggatg acttgggaaa gggttgagtc
     8041 ttttgtgagt cttgcggagg cccaggaggg ggtacgtaaa ttagagttct aagaggccca
     8101 gcaaaagtga acaagtgact ggccactgtg agttcactgg gggacctgag agttaactag
     8161 tgaaaaggag aggtcctagt ggagaagcta aagaattaaa gttactgcct tgaagttctt
     8221 tgtgaaggcc aagctctgga gcgcggcttg aaaagccttt atcccagagc agcatgggaa
     8281 ggggccccag ctgggtgcga gcctgcaaac cagagcacac ctatcacagg gccttttctt
     8341 accagtggga cgggatcaag gcggagctta cctgggaacg tacagggcgg aaccttaact
     8401 tgggggcgtg gccgaatcac ctgggtttgg aaacggttct tgtctttcct caagctcagg
     8461 agaggttgtt tgcaggacac tggcccggag gggcccgaac tctccgaggg agggtttcgt
     8521 ggatcagaac accgtccctt gacaaaaaga tgcagagcac cgtctgcatt ctgctagtaa
     8581 ccgtccctcc tgggggcgga gccagccctc ctccgggtgt ataaagtagc tgagcgcctc
     8641 cttctgacaa acgcctcacc cccaagagta ctgggggagt acgcagcacg ccagcctagg
     8701 ggcggagctc agccctggag gaggcgtttc ttcttagttc tccgactcac gcctctccac
     8761 ttttcattta aatggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8821 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8881 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8941 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9001 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9061 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9121 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9181 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9241 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9301 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9361 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9421 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9481 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9541 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9601 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9661 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9721 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9781 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9841 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9901 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9961 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10021 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10081 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10141 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10201 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10261 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10321 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10381 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10441 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10501 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10561 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10621 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10681 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10741 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10801 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10861 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10921 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    10981 ataggcgtat cacgaggccc tttcgtc
//
