LOCUS       sequence-34906-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Kamura T, Sato S, Iwai K, Czyzyk-Krzeska M, Conaway RC, Conaway JW
  TITLE     Activation of HIF1alpha ubiquitination by a reconstituted von 
            Hippel-Lindau (VHL) tumor suppressor complex.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Sep 12. 97(19):10430-5.
  PUBMED    10973499
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Sep 12. 97(19):10430-5."
COMMENT     Sequence Label: pBacPAK8-His-myc-Rbx1
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             58..80
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             133..162
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 taaaaaaacc tataaatacg gatccctgca ggcctcgagt tcgaatctag aaataatttt
       61 gtttaacttt aagaaggaga tatacatatg cggggttctc atcatcatca tcatcatggt
      121 atggctagca tggagcagaa gctgatcagc gaggaggacc tgatggcggc ggcgatggat
      181 gtggataccc ccagcggcac caacagcggc gcgggcaaga agcgctttga agttaaaaag
      241 tggaatgcag tggccctctg ggcctgggac attgtggttg ataactgtgc catctgcagg
      301 aaccacatta tggatctttg tatcgaatgt caggccaacc aggcgtcagc tacttccgaa
      361 gagtgtacgg ttgcatgggg agtctgcaac catgcttttc atttccactg catctctcga
      421 tggctcaaaa cgaggcaggt gtgtccgttg gacaacagag agtgggagtt ccagaagtat
      481 gggcattagg aaagcttgat ccggctgctc gagaattccc gggcggccgc ttaattaatt
      541 gatccgggtt attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac
      601 aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga
      661 gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata
      721 gatttgtaaa ataggtttcg attagtttca aaca
//
