LOCUS       sequence-34908-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Kamura T, Sato S, Iwai K, Czyzyk-Krzeska M, Conaway RC, Conaway JW
  TITLE     Activation of HIF1alpha ubiquitination by a reconstituted von 
            Hippel-Lindau (VHL) tumor suppressor complex.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Sep 12. 97(19):10430-5.
  PUBMED    10973499
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Sep 12. 97(19):10430-5."
COMMENT     Sequence Label: pBacPAK8-His-myc-Uba1
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             67..89
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             109..126
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gttttgtaat aaaaaaacct ataaatacgg atccctgcag gcctcgagtt cgaatctaga
       61 aataattttg tttaacttta agaaggagat atacatatgc ggggttctca tcatcatcat
      121 catcatggta tggctagcat ggagcagaag ctgatcagcg aggaggacct gatgagcagc
      181 aataatagtg gtctctctgc cgccggagaa atcgatgaaa gtctttattc tcgtcaactt
      241 tatgtgttgg gtaaggaagc aatgttgaaa atgcaaacct ccaacgtatt aattctcggc
      301 ttgaaggggc taggtgttga gatagcgaag aacgttgtgt tggcaggtgt caaatctatg
      361 accgtttttg accctgagcc ggttcaattg gcagacttat caacccaatt ctttttgacc
      421 gaaaaggata ttggtcaaaa gagaggagat gtgaccagag ccaaattggc agagttgaac
      481 gcatatgtcc cggttaatgt gctggattcc ttagacgatg ttacccaatt gagtcaattc
      541 caggtcgttg tcgctacgga cactgtttct ttggaagata aggtgaaaat caacgaattt
      601 tgccattcct ccggtattag attcatttcc tcggagacaa gagggctgtt tggtaacacc
      661 ttcgtagacc taggtgacga gttcacagtg ttggacccaa cgggtgaaga acctcgcact
      721 ggtatggttt cagacatcga gcccgatgga acagtgacca tgctagatga taacagacac
      781 gggttggaag acggaaattt tgtcaggttc tccgaagtag aaggtcttga taagttgaat
      841 gatgggaccc tttttaaagt tgaggttttg gggccctttg cattcagaat tggttcagtc
      901 aaagaatatg gcgagtataa gaagggcggt atctc
//
