LOCUS       sequence-349111-        9730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3180
FEATURES             Location/Qualifiers
     source          1..9730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2668..3256
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2721..2741)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3139..3150)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3328..3561
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3639..3760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3676..3695)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3730..3749
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3800..3935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3955..3974)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3956..3974)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3984..4001)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3984..4000)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3993..4015)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4142..4597
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4229..4248)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4439..4460
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4623..4727
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4728..4796,4797..5588)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4946..4965)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5759..6347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6248..6267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6501..6518
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6635..6656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6671..6676,6677..6694,6695..6701)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6709..6725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6714..6736
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6733..6749
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6733..6749
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6768..6788
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6770..6788
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6816..7042
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7043..7223
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7270..7395
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7888..8121
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8306..8350
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8581..8791
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8603..8799
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8650..8785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8831..9433
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8831..8850)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9327..9347
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9476..9593
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 caccaataag gagcgactct ttgcggctgg tgccctgggg cctggatctg gctacccagg
     1261 ggcaggtttc cccttcgcct tcccaggggc actcaggggg tctccgcctt tcgagatgct
     1321 gagccctagc ttccggggcc tgggccagcc tgacctcccc aaggagatgg cctctctgtc
     1381 ggtggagaca cagagcacca gctcagagga gatggtgccc agctcgccct cgccccctcc
     1441 gcctcctcgg gtctacaagc catgcttcgt gtgcaatgac aagtcctctg gctaccacta
     1501 tggggtcagc tcttgtgaag gctgcaaggg cttctttcgc cgaagcatcc agaagaacat
     1561 ggtgtacacg tgtcaccgcg acaaaaactg tatcatcaac aaggtgacca ggaatcgctg
     1621 ccagtactgc cggctacaga agtgcttcga agtgggcatg tccaaggaag ctgtgcgaaa
     1681 tgaccggaac aagaagaaga aagaggtgaa ggaagaaggg tcacctgaca gctatgagct
     1741 gagccctcag ttagaagagc tcatcaccaa ggtcagcaaa gcccatcagg agactttccc
     1801 ctcgctctgc cagctgggca agtataccac gaactccagt gcagaccacc gcgtgcagct
     1861 ggatctgggg ctgtgggaca agttcagtga gctggctacc aagtgcatca tcaagatcgt
     1921 ggagtttgcc aagcggttgc ctggctttac agggctcagc attgctgacc agatcactct
     1981 gctcaaagct gcctgcctag atatcctgat gctgcgtatc tgcacaaggt acaccccaga
     2041 gcaggacacc atgaccttct ccgacgggct gaccctgaac cggacccaga tgcacaatgc
     2101 cggcttcggg cccctcacag accttgtctt tgcctttgct gggcagctcc tgcccctgga
     2161 gatggatgac accgagacag ggctgctcag cgccatctgc ctcatctgcg gagaccgcat
     2221 ggacctggag gagcccgaaa aagtggacaa gctgcaggag ccactgctgg aagccctgag
     2281 gctgtacgcc cggcgccggc ggcccagcca gccctacatg ttcccaagga tgctaatgaa
     2341 aatcaccgac ctccggggca tcagcactaa gggagctgaa agggccatta ctctgaagat
     2401 ggagattcca ggcccgatgc ctcccttaat ccgagagatg ctggagaacc ctgaaatgtt
     2461 tgaggatgac tcctcgcagc ctggtcccca ccccaatgcc tctagcgagg atgaggttcc
     2521 tgggggccag ggcaaagggg gcctgaagtc cccagcctag taatgaacta gtccgtaact
     2581 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa atcgctcgat
     2641 atgcaaccga caacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     2701 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2761 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2821 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2881 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2941 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3001 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3061 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3121 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3181 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3241 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     3301 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3361 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     3421 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3481 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     3541 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     3601 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     3661 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3721 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     3781 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3841 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3901 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     3961 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4021 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4081 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4141 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4201 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4261 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4321 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4381 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4441 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4501 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4561 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     4621 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4681 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4741 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4801 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4861 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4921 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4981 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5041 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5101 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5161 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5221 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5281 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5341 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5401 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5461 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5521 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5581 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5641 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5701 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5761 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5821 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5881 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5941 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6001 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6061 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6121 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6181 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6241 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6301 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6361 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6421 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6481 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6541 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6601 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     6661 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     6721 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     6781 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     6841 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     6901 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     6961 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     7021 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     7081 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7141 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7201 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7261 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7321 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7381 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7441 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     7501 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     7561 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     7621 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     7681 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     7741 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     7801 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     7861 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     7921 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     7981 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8041 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8101 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8161 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8221 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8281 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8341 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8401 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     8461 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     8521 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8581 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
     8641 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8701 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8761 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
     8821 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     8881 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     8941 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9001 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     9061 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9121 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9181 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9241 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9301 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9361 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9421 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     9481 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9541 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9601 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg
     9661 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
     9721 agtgggaatt
//
