LOCUS       sequence-34919-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Jin M, Smith C, Hsieh HY, Gibson DF, Tait JF
  TITLE     Essential role of B-helix calcium binding sites in annexin 
            V-membrane binding
  JOURNAL   J Biol Chem 2004; 279:40351-57.
  PUBMED    15280367
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem"; date: "2004"; volume: " 279"; pages: "40351-57"
COMMENT     Sequence Label: pJ143
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(27..54)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(146..232)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        join(402..407,408..424,425..430)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 aataacaaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttaa
       61 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg
      121 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
      181 aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg
      241 cagttcccta ctctcgcatg gggagacccc acactaccat cggcgctacg gcgtttcact
      301 tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg caaattctgt
      361 tttatcagcc taggcccttt cgtcttcaag aattatcatg tttgacagct tatcatcgaa
      421 tagctttaat gcggtagttt atcacagtta aattgctaac gcagtcagga gtattgatta
      481 tgactatgat tacaacgccc gggcaggaat tcgatttagt catcttctcc agagagcagc
      541 agaagagctt tcttatagtc cccagatgta tttcccttaa tcatggaata aagagaggtg
      601 gcaaaattct tcctaaactc cttcctgatg ttaaacagat caatctcact cctggaaacc
      661 atgactctga tgagggtatg atcatctgtc ccagctccct tcatagcata atagagggtc
      721 tctgcaaggt aggcaggtat acttcgaata gatttcacaa cagcaaggag tagttgctct
      781 aaattgccag aagtctcgcg gtcaatggtt tcctcaattt gaaatcctga tatagtcatg
      841 tacttgtcaa acacctttct caaatgagac ac
//
