LOCUS       sequence-349207-        7439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conditionally active form of Cre recombinase (activated in response 
            to tamoxifen) under Syn promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Linghu C, An B, Shpokayte M, Celiker OT, Shmoel N, Zhang R, Zhang C,
            Park D, Park WM, Ramirez S, Boyden ES
  TITLE     Recording of cellular physiological histories along optically 
            readable self-assembling protein chains.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01586-7.
  PUBMED    36593405
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01586-7."
COMMENT     Sequence Label: pAAV-Syn-ERT2-iCre-ERT2
FEATURES             Location/Qualifiers
     source          1..7439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   58..187
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    243..291
                     /note="synthetic polyadenylation signal"
     misc_feature    305..396
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     345..364
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        432..879
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      888..897
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             897..1832
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2877..3812
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2925..3818
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     misc_feature    3843..4431
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3896..3916)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4314..4325)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4464..4481)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4470..4677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4686..4815
                     /label=AAV2 ITR
     rep_origin      4924..5379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5011..5030)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5221..5242
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5446..5465)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5565..5587
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5625..5643)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5711..5815
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5816..5884,5885..6676)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6034..6053)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6847..7435
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7336..7355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtcct
       61 gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc gacctttggt
      121 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
      181 ggttcctgcg gccgcacgcg ttctagaaat attaaggtac gggaggtact tggagcggcc
      241 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
      301 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
      361 tccccagtgc aagtgcaggt gccagaacat ttctctattc tagactcgag ctgcagaggg
      421 ccctgcgtat gagtgcaagt gggttttagg accaggatga ggcggggtgg gggtgcctac
      481 ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca aattgcgcat
      541 cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc actgccagct
      601 tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc
      661 actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc cggccacctt
      721 ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg cgagataggg
      781 gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg
      841 gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt cgaattcgcc accatggctg
      901 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga
      961 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
     1021 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg
     1081 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
     1141 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc
     1201 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt
     1261 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
     1321 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
     1381 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
     1441 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag
     1501 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
     1561 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc
     1621 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg
     1681 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg
     1741 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca
     1801 tcacggggga ggcagagggt ttccctgcca cagctgtcga caacctgctg actgtgcacc
     1861 aaaacctgcc tgccctccct gtggatgcca cctctgatga agtcaggaag aacctgatgg
     1921 acatgttcag ggacaggcag gccttctctg aacacacctg gaagatgctc ctgtctgtgt
     1981 gcagatcctg ggctgcctgg tgcaagctga acaacaggaa atggttccct gctgaacctg
     2041 aggatgtgag ggactacctc ctgtacctgc aagccagagg cctggctgtg aagaccatcc
     2101 aacagcacct gggccagctc aacatgctgc acaggagatc tggcctgcct cgcccttctg
     2161 actccaatgc tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg
     2221 agagagccaa gcaggccctg gcctttgaac gcactgactt tgaccaagtc agatccctga
     2281 tggagaactc tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca
     2341 acaccctgct gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg
     2401 atggtgggag aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg
     2461 tggagaaggc cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg
     2521 gtgtggctga tgaccccaac aactacctgt tctgccgggt cagaaagaat ggtgtggctg
     2581 ccccttctgc cacctcccaa ctgtccaccc gggccctgga agggatcttt gaggccaccc
     2641 accgcctgat ctatggtgcc aaggatgact ctgggcagag atacctggcc tggtctggcc
     2701 actctgccag agtgggtgct gccagggaca tggccagggc tggtgtgtcc atccctgaaa
     2761 tcatgcaggc tggtggctgg accaatgtga acattgtgat gaactacatc agaaacctgg
     2821 actctgagac tggggccatg gtgaggctgc tcgaggatgg ggacctcgag ccatctgctg
     2881 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga
     2941 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
     3001 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg
     3061 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
     3121 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc
     3181 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt
     3241 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
     3301 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
     3361 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
     3421 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag
     3481 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
     3541 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc
     3601 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg
     3661 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg
     3721 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca
     3781 tcacggggga ggcagagggt ttccctgcca cagcttgagc tagcgatatc aagcttatcg
     3841 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3901 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3961 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4021 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4081 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4141 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4201 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     4261 tcgcctgtgt tgccacctgg attctgcgcg ggacgccctt ctgctacgtc ccttcggccc
     4321 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4381 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     4441 gtcgactaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4501 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4561 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4621 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggagag
     4681 atctgaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     4741 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     4801 cgagcgagcg cgcagagagg gagtggcccc cccccccccc ccccccctgc agcctggcgt
     4861 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4921 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4981 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5041 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5101 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5161 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5221 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5281 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5341 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct
     5401 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     5461 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5521 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     5581 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     5641 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5701 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5761 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5821 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5881 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5941 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6001 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6061 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6121 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6181 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6241 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6301 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6361 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6421 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6481 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6541 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6601 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6661 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6721 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6781 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6841 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6901 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6961 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7021 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7081 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7141 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7201 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7261 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7321 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7381 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacg
//
