LOCUS       sequence-349228-        7121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conditionally active form of Cre recombinase (activated in response 
            to tamoxifen) under UBC promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Linghu C, An B, Shpokayte M, Celiker OT, Shmoel N, Zhang R, Zhang C,
            Park D, Park WM, Ramirez S, Boyden ES
  TITLE     Recording of cellular physiological histories along optically 
            readable self-assembling protein chains.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01586-7.
  PUBMED    36593405
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01586-7."
COMMENT     Sequence Label: pAAV-UBC-ERT2-iCre-ERT2
FEATURES             Location/Qualifiers
     source          1..7121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   72..201
                     /label=AAV2 ITR
     promoter        274..672
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      681..690
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             690..1625
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2670..3605
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2718..3611
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     misc_feature    3636..4224
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3689..3709)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4107..4118)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4267..4388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4278..4297)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4332..4351
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4550..4679
                     /label=AAV2 ITR
     rep_origin      complement(4916..5504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4996..5015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5675..6466,6467..6535))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6298..6317
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6536..6640)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6666..7121)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6803..6824)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7015..7034
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
      121 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      181 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
      241 agccatgctc taggaagatc gtaggttaat taaggcctcc gcgccgggtt ttggcgcctc
      301 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
      361 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
      421 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
      481 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
      541 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
      601 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
      661 atcgtcactt ggcgactagt gccaccatgg ctggagacat gagagctgcc aacctttggc
      721 caagcccgct catgatcaaa cgctctaaga agaacagcct ggccttgtcc ctgacggccg
      781 accagatggt cagtgccttg ttggatgctg agccccccat actctattcc gagtatgatc
      841 ctaccagacc cttcagtgaa gcttcgatga tgggcttact gaccaacctg gcagacaggg
      901 agctggttca catgatcaac tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc
      961 atgatcaggt ccaccttcta gaatgtgcct ggctagagat cctgatgatt ggtctcgtct
     1021 ggcgctccat ggagcaccca gtgaagctac tgtttgctcc taacttgctc ttggacagga
     1081 accagggaaa atgtgtagag ggcatggtgg agatcttcga catgctgctg gctacatcat
     1141 ctcggttccg catgatgaat ctgcagggag aggagtttgt gtgcctcaaa tctattattt
     1201 tgcttaattc tggagtgtac acatttctgt ccagcaccct gaagtctctg gaagagaagg
     1261 accatatcca ccgagtcctg gacaagatca cagacacttt gatccacctg atggccaagg
     1321 caggcctgac cctgcagcag cagcaccagc ggctggccca gctcctcctc atcctctccc
     1381 acatcaggca catgagtaac aaaggcatgg agcatctgta cagcatgaag tgcaagaacg
     1441 tggtgcccct ctatgacctg ctgctggagg cggcggacgc ccaccgccta catgcgccca
     1501 ctagccgtgg aggggcatcc gtggaggaga cggaccaaag ccacttggcc actgcgggct
     1561 ctacttcatc gcattccttg caaaagtatt acatcacggg ggaggcagag ggtttccctg
     1621 ccacagctgt cgacaacctg ctgactgtgc accaaaacct gcctgccctc cctgtggatg
     1681 ccacctctga tgaagtcagg aagaacctga tggacatgtt cagggacagg caggccttct
     1741 ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc
     1801 tgaacaacag gaaatggttc cctgctgaac ctgaggatgt gagggactac ctcctgtacc
     1861 tgcaagccag aggcctggct gtgaagacca tccaacagca cctgggccag ctcaacatgc
     1921 tgcacaggag atctggcctg cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga
     1981 ggagaatcag aaaggagaat gtggatgctg gggagagagc caagcaggcc ctggcctttg
     2041 aacgcactga ctttgaccaa gtcagatccc tgatggagaa ctctgacaga tgccaggaca
     2101 tcaggaacct ggccttcctg ggcattgcct acaacaccct gctgcgcatt gccgaaattg
     2161 ccagaatcag agtgaaggac atctcccgca ccgatggtgg gagaatgctg atccacattg
     2221 gcaggaccaa gaccctggtg tccacagctg gtgtggagaa ggccctgtcc ctgggggtta
     2281 ccaagctggt ggagagatgg atctctgtgt ctggtgtggc tgatgacccc aacaactacc
     2341 tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc caactgtcca
     2401 cccgggccct ggaagggatc tttgaggcca cccaccgcct gatctatggt gccaaggatg
     2461 actctgggca gagatacctg gcctggtctg gccactctgc cagagtgggt gctgccaggg
     2521 acatggccag ggctggtgtg tccatccctg aaatcatgca ggctggtggc tggaccaatg
     2581 tgaacattgt gatgaactac atcagaaacc tggactctga gactggggcc atggtgaggc
     2641 tgctcgagga tggggacctc gagccatctg ctggagacat gagagctgcc aacctttggc
     2701 caagcccgct catgatcaaa cgctctaaga agaacagcct ggccttgtcc ctgacggccg
     2761 accagatggt cagtgccttg ttggatgctg agccccccat actctattcc gagtatgatc
     2821 ctaccagacc cttcagtgaa gcttcgatga tgggcttact gaccaacctg gcagacaggg
     2881 agctggttca catgatcaac tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc
     2941 atgatcaggt ccaccttcta gaatgtgcct ggctagagat cctgatgatt ggtctcgtct
     3001 ggcgctccat ggagcaccca gtgaagctac tgtttgctcc taacttgctc ttggacagga
     3061 accagggaaa atgtgtagag ggcatggtgg agatcttcga catgctgctg gctacatcat
     3121 ctcggttccg catgatgaat ctgcagggag aggagtttgt gtgcctcaaa tctattattt
     3181 tgcttaattc tggagtgtac acatttctgt ccagcaccct gaagtctctg gaagagaagg
     3241 accatatcca ccgagtcctg gacaagatca cagacacttt gatccacctg atggccaagg
     3301 caggcctgac cctgcagcag cagcaccagc ggctggccca gctcctcctc atcctctccc
     3361 acatcaggca catgagtaac aaaggcatgg agcatctgta cagcatgaag tgcaagaacg
     3421 tggtgcccct ctatgacctg ctgctggagg cggcggacgc ccaccgccta catgcgccca
     3481 ctagccgtgg aggggcatcc gtggaggaga cggaccaaag ccacttggcc actgcgggct
     3541 ctacttcatc gcattccttg caaaagtatt acatcacggg ggaggcagag ggtttccctg
     3601 ccacagcttg agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     3661 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3721 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3781 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3841 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3901 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3961 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4021 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4081 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4141 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4201 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc cgggcggccg cttcgagcag
     4261 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     4321 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     4381 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     4441 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatcttccta
     4501 gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta
     4561 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     4621 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     4681 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4801 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5341 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5461 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5521 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5581 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5641 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5701 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5761 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5821 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5881 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5941 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6001 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6061 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6121 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6181 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6241 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6301 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6361 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6421 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6481 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6541 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6601 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6661 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     6721 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     6781 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     6841 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     6901 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     6961 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     7021 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     7081 caccacaccc gccgcgctta atgcgccgct acagggcgcg t
//
