LOCUS       sequence-349280-        5968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfer vector to generate recombinant baculovirus expressing 
            BAF57/SMARCE1-His protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Shimoda M, Lyu Y, Wang KH, Kumar A, Miura H, Meckler JF, Davis RR, 
            Chantarasrivong C, Izumiya C, Tepper CG, Nakajima KI, Tuscano J, 
            Barisone G, Izumiya Y
  TITLE     KSHV transactivator-derived small peptide traps coactivators to 
            attenuate MYC and inhibits leukemia and lymphoma cell growth.
  JOURNAL   Commun Biol. 2021 Dec 2;4(1):1330. doi: 10.1038/s42003-021-02853-0.
  PUBMED    34857874
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-2"; volume: "4(1):1330. doi"; pages: " 
            10.1038/s42003-021-02853-0"
COMMENT     Sequence Label: pFAST-BAC BAF57/SMARCE1-His
FEATURES             Location/Qualifiers
     source          1..5968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(430..435,436..452,453..458))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        810..901
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     826..843
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2190..2207
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2364..2498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2401..2420)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2455..2474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2527..2692
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2876..3330
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2963..2982)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3172..3193
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3356..3460
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3461..3529,3530..4321)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3679..3698)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4492..5080
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4981..5000
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5234..5251
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5385..5609
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(5676..5968,1..241))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 cagcaagcgc gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct
       61 gatgttggga gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc
      121 gcatggattt gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc
      181 cacctaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca
      241 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
      301 gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
      361 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
      421 ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt
      481 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
      541 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
      601 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
      661 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
      721 ggttttctgg aaggcgagca tcgtttgttc gcccaggact ctagctatag ttctagtggt
      781 tggctacgta tactccggaa tattaataga tcatggagat aattaaaatg ataaccatct
      841 cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa aacctataaa
      901 tattccggat tattcatacc gtcccaccat cgggcgcgac catgtcaaaa agaccatctt
      961 atgccccacc tcccacccca gctcctgcaa cacaaatgcc cagcacacca gggtttgtgg
     1021 gatacaatcc atacagtcat ctcgcctaca acaactacag gctgggaggg aacccgggca
     1081 ccaacagccg ggtcacggca tcctctggta tcacgattcc aaaaccccca aagccaccag
     1141 ataagccgct gatgccctac atgaggtaca gcagaaaggt ctgggaccaa gtaaaggctt
     1201 ccaaccctga cctaaagttg tgggagattg gcaagattat tggtggcatg tggcgagatc
     1261 tcactgatga agaaaaacaa gaatatttaa acgaatacga agcagaaaag atagagtaca
     1321 atgaatctat gaaggcctat cataattccc ccgcgtacct tgcttacata aatgcaaaaa
     1381 gtcgtgcaga agctgcttta gaggaagaaa gtcgacagag acaatctcgc atggagaaag
     1441 gagaaccgta catgagcatt cagcctgctg aagatccaga tgattatgat gatggctttt
     1501 caatgaagca tacagccacc gcccgtttcc agagaaacca ccgcctcatc agtgaaattc
     1561 ttagtgagag tgtggtgcca gacgttcggt cagttgtcac aacagctaga atgcaggtcc
     1621 tcaaacggca ggtccagtcc ttaatggttc atcagcgaaa actagaagct gaacttcttc
     1681 aaatagagga acgacaccag gagaagaaga ggaaattcct ggaaagcaca gattcattta
     1741 acaatgaact taaaaggttg tgcggtctga aagtagaagt ggatatggag aaaattgcag
     1801 ctgagattgc acaggcagag gaacaggccc gcaaaaggca ggaggaaagg gagaaggagg
     1861 ccgcagagca agctgagcgc agtcagagca gcatcgttcc tgaggaagaa caagcagcta
     1921 acaaaggcga ggagaagaaa gacgacgaga acattccgat ggagacagag gagacacacc
     1981 ttgaagaaac aacagagagc caacagaatg gtgaagaagg cacgtctact cctgaggaca
     2041 aggagagtgg gcaggagggg gtcgacagta tggcagagga aggaaccagt gatagtaaca
     2101 ctggctcgga gagcaacagt gcaacagtgg aggagccacc aacagatccc ataccagaag
     2161 atgagaaaaa agaaggcgga ggaggttcac atcatcatca tcaccactaa tgcagtctcg
     2221 aggcatgcgg taccaagctt gtcgagaagt actagaggat cataatcagc cataccacat
     2281 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2341 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2401 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2461 tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagga
     2521 gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt aattttcgta
     2581 ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct
     2641 taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca
     2701 tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga caaaccgtca
     2761 tcttcggcta ctttttctct gtcacagaat gaaaattttt ctgtcatctc ttcgttatta
     2821 atgtttgtaa ttgactgaat atcaacgctt atttgcagcc tgaatggcga atgggacgcg
     2881 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2941 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3001 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3061 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3121 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttcttaat agtggactct
     3181 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3241 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3301 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     3361 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3421 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3481 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3541 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3601 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3661 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3721 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3781 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3841 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3901 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3961 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4021 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4081 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4141 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4201 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4261 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4321 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4381 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4441 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4501 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4561 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4621 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4681 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4741 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4801 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4861 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4921 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4981 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5041 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5101 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5161 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5221 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     5281 tctccttacg catctgtgcg gtatttcaca ccgcatagac cagccgcgta acctggcaaa
     5341 atcggttacg gttgagtaat aaatggatgc cctgcgtaag cgggtgtggg cggacaataa
     5401 agtcttaaac tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa
     5461 tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat
     5521 ggctaaagca aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg
     5581 tggccaaggg catggtaaag actatattcg cggcgttgtg acaatttacc gaacaactcc
     5641 gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata
     5701 tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg
     5761 tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg
     5821 aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga
     5881 tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga
     5941 gcgccaacaa ccgcttcttg gtcgaagg
//
