LOCUS       sequence-349281-        8050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfer vector to generate recombinant baculovirus expressing 
            BAF155/SMARCC1-His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Shimoda M, Lyu Y, Wang KH, Kumar A, Miura H, Meckler JF, Davis RR, 
            Chantarasrivong C, Izumiya C, Tepper CG, Nakajima KI, Tuscano J, 
            Barisone G, Izumiya Y
  TITLE     KSHV transactivator-derived small peptide traps coactivators to 
            attenuate MYC and inhibits leukemia and lymphoma cell growth.
  JOURNAL   Commun Biol. 2021 Dec 2;4(1):1330. doi: 10.1038/s42003-021-02853-0.
  PUBMED    34857874
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-2"; volume: "4(1):1330. doi"; pages: " 
            10.1038/s42003-021-02853-0"
COMMENT     Sequence Label: pFAST-BAC BAF155/SMARCC1-His
FEATURES             Location/Qualifiers
     source          1..8050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(115..120,121..137,138..143))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        495..586
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     511..528
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3957..3974
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4131..4265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4168..4187)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4222..4241
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4294..4459
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4643..5097
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4730..4749)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4939..4960
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5123..5227
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5228..5296,5297..6088)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5446..5465)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6259..6847
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6748..6767
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7001..7018
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  7152..7376
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7443..7976)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
       61 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
      121 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
      181 cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag
      241 cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc
      301 aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg
      361 cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc
      421 gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct acgtatactc
      481 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
      541 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
      601 ataccgtccc accatcgggc gcgaccatgg ccgcagcggc gggcggcggc gggccgggga
      661 cagcggtagg cgccacgggc tcggggattg cggcggcagc cgcaggccta gctgtttatc
      721 gacggaagga tgggggcccg gccaccaagt tttgggagag cccggagacg gtgtcccagc
      781 tggattcggt gcgggtctgg ctgggcaagc actacaagaa gtatgttcat gcggatgctc
      841 ctaccaataa aacactggct gggctggtgg tgcagcttct tcagttccag gaagatgcct
      901 ttgggaagca tgtcaccaac ccggccttca ccaaactccc tgcaaagtgt ttcatggatt
      961 tcaaagctgg aggcgcctta tgtcacattc ttggggctgc ttacaagtat aaaaatgaac
     1021 agggatggcg gaggtttgac ctacagaacc catctcgaat ggatcgtaat gtggaaatgt
     1081 ttatgaacat tgaaaaaaca ttggtgcaga acaattgttt gaccagaccc aacatctacc
     1141 tcattccaga cattgatctg aagttggcta acaaattgaa agatatcatc aaacgacatc
     1201 agggaacatt tacggatgag aagtcaaaag cttcccacca catttaccca tattcttcct
     1261 cacaagacga tgaagaatgg ttgagaccgg tgatgagaaa agagaagcaa gtgttagtgc
     1321 attggggctt ttacccagac agctatgata cttgggtcca tagtaatgat gttgatgctg
     1381 aaattgaaga tccaccaatt ccagaaaaac catggaaggt tcatgtgaaa tggattttgg
     1441 acactgatat tttcaatgaa tggatgaatg aggaggatta tgaggtggat gaaaatagga
     1501 agcctgtgag ttttcgtcag cggatttcaa ccaagaatga agagccagtc agaagtccag
     1561 aaagaagaga tagaaaagca tcagctaatg ctcgaaagag gaaacattcg ccttcgcctc
     1621 cccctccgac accaacagaa tcacggaaga agagtgggaa gaaaggccaa gctagccttt
     1681 atgggaagcg cagaagtcag aaagaggaag atgagcaaga agatctaacc aaggatatgg
     1741 aagacccaac acctgtaccc aatatagaag aagtagtact tcccaaaaat gtgaacctaa
     1801 agaaagatag tgaaaataca cctgttaaag gaggaactgt agcggatcta gatgagcagg
     1861 atgaagaaac agtcacagca ggaggaaagg aagatgaaga tcctgccaaa ggtgatcaga
     1921 gtcgatcagt tgaccttggg gaagataatg tgacagagca gaccaatcac attattattc
     1981 ctagttatgc atcatggttt gattataact gtattcatgt gattgaacgg cgtgctcttc
     2041 ctgagttctt caatggaaaa aacaaatcca agactccaga aatatacttg gcatatcgaa
     2101 attttatgat tgacacgtat cgtctaaacc cccaagagta tttaactagc actgcttgtc
     2161 ggaggaactt gactggagat gtgtgtgctg tgatgagggt ccatgccttt ttagagcagt
     2221 ggggactcgt taattaccaa gttgacccgg aaagtagacc catggcaatg ggacctcctc
     2281 ctactcctca ttttaatgta ttagctgata ccccctctgg gcttgtgcct ctgcatcttc
     2341 gatcacctca ggttcctgct gctcaacaga tgctaaattt tcctgagaaa aacaaggaaa
     2401 aaccagttga tttgcagaac tttggtctcc gtactgacat ttactccaag aaaacattag
     2461 caaagagtaa aggtgctagt gctggaagag aatggactga acaggagacc cttctactcc
     2521 tggaggccct ggagatgtac aaggatgatt ggaacaaagt gtcggaacat gttggaagtc
     2581 gtactcagga tgaatgcatc ctccactttt tgagacttcc cattgaggac ccataccttg
     2641 agaattcaga tgcttccctt gggcctttgg cctaccagcc tgtccccttc agtcagtcag
     2701 gaaatccagt tatgagtact gttgcttttt tggcatctgt ggtggaccct cgcgtggcat
     2761 ctgctgcagc aaaagcggct ttggaggagt tttctcgggt ccgggaggag gtaccactgg
     2821 aattggttga agctcatgtc aagaaagtac aagaagcagc acgagcctct gggaaagtgg
     2881 atcccaccta cggtctggag agcagctgca ttgcaggcac agggcccgat gagccagaga
     2941 agcttgaagg agctgaagag gaaaaaatgg aagccgaccc tgatggtcag cagcctgaaa
     3001 aggcagaaaa taaagtggaa aatgaaacgg atgaaggtga taaagcacaa gatggagaaa
     3061 atgaaaaaaa tagtgaaaag gaacaggata gtgaagtgag tgaggatacc aaatcagaag
     3121 aaaaggagac tgaagagaac aaagaactca ctgatacatg taaagaaaga gaaagtgata
     3181 ctgggaagaa gaaagtagaa catgaaattt ccgaaggaaa tgttgccaca gccgcagcag
     3241 ctgctcttgc ctcagcggct accaaagcca agcacctggc tgcagtggaa gaaagaaaga
     3301 tcaagtccct ggtagctctc ttggttgaga cacaaatgaa gaaactagag atcaaacttc
     3361 gacattttga agagctggaa actatcatgg acagagagaa agaagctcta gaacaacaga
     3421 ggcagcagtt gcttactgaa cgccaaaact tccacatgga acagctgaag tatgctgaat
     3481 tacgagcacg acagcaaatg gaacagcagc agcatggcca gaaccctcaa caggcacacc
     3541 agcactcagg aggacctggc ctggccccac ttggagcagc agggcaccct ggcatgatgc
     3601 ctcatcaaca gccccctccc taccctctga tgcaccacca gatgccacca cctcatccac
     3661 cccagccagg tcagatacca ggcccaggtt ccatgatgcc cgggcagcac atgccaggcc
     3721 gcatgattcc cactgttgca gccaacatcc acccctctgg gagtggccct acccctcctg
     3781 gcatgccacc aatgccagga aacatcttag gaccccgggt acccctgaca gcacctaacg
     3841 gcatgtatcc ccctccacca cagcagcagc caccgccacc accacctgca gatggggtcc
     3901 ctccgcctcc tgctcctggc ccgccagcct cagctgctcc tggcggagga ggttcacatc
     3961 atcatcatca ccactaatgc agtctcgagg catgcggtac caagcttgtc gagaagtact
     4021 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     4081 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     4141 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4201 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4261 ggatctgatc actgcttgag cctaggagat ccgaaccaga taagtgaaat ctagttccaa
     4321 actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta
     4381 ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca
     4441 actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta atcaaacatc
     4501 ctgccaactc catgtgacaa accgtcatct tcggctactt tttctctgtc acagaatgaa
     4561 aatttttctg tcatctcttc gttattaatg tttgtaattg actgaatatc aacgcttatt
     4621 tgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4681 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4741 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4801 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4861 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4921 gagtccacgt tcttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4981 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5041 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     5101 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5161 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5221 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5281 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5341 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5401 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5461 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5521 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5581 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5641 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5701 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5761 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5821 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5881 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5941 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6001 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6061 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6121 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6181 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6241 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6301 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6361 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6421 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6481 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6541 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6601 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6661 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6721 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6781 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6841 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6901 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6961 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7021 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     7081 catagaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa tggatgccct
     7141 gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga tctaaactat
     7201 gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc agttatgctg
     7261 tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt ctgaagtgca
     7321 aattgcccgt cgtattaaag aggggcgtgg ccaagggcat ggtaaagact atattcgcgg
     7381 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     7441 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     7501 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     7561 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     7621 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     7681 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     7741 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     7801 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     7861 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     7921 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     7981 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     8041 aggcatagac
//
