LOCUS       sequence-349327-        4374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human TARDBP at the 
            N-terminus with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4374)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TARDBP_Halo_N_allele
FEATURES             Location/Qualifiers
     source          1..4374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..30)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    19..35
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(43..49,50..67,68..73))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(226..243)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(397..985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(477..496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1156..1947,1948..2016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1779..1798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2017..2121)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2189..2207
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2245..2267)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2367..2386
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2580..2602
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2594..2611
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2595..2611
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3018..3908
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     primer_bind     complement(join(4369..4374,1..11))
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(4369..4374,1..11))
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
       61 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      121 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      181 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1141 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1201 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1261 cttaccatct ggccccagtg ctgcaatgat accgcgagat ccacgctcac cggctccaga
     1321 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1381 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1441 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1501 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1561 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     1621 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     1681 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     1741 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     1801 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     1861 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     1921 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     1981 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2041 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2101 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     2161 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     2221 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     2281 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2341 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     2401 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     2461 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2521 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2581 ccagtcacga cgttgtaaaa cgacggccag tgaattcagc cagttaggct cctcctctgc
     2641 cttcaggatt acttaacgac taacccgatt gtcataaaat agctactgtt tattgttccc
     2701 ttgcataccc tagcttgtaa ccagcaagcc acgttggact cacagttaca gtttcagtac
     2761 ctgacgcatc ataagccttc agggaaagtt ttttgaatga atgaactggc gaggcatcac
     2821 attttgatag gaaatcacta cccttacctt cacctcgtca tttttcaggg ataaccaatg
     2881 catatacgaa tccagacaag catttttctg gaagtcagaa ctctgacatg gtttgggtat
     2941 tatcattata acgaaacagt tattctgaca tgaatgttgt tcattcatat ctcttttctc
     3001 tttaggaaaa gtaaaagatg gccgagatcg gcaccggctt tcccttcgat cctcactacg
     3061 tggaagtgct gggcgagaga atgcactatg tggacgtggg ccccagagat ggaacccctg
     3121 tgctgtttct gcacggcaac cctaccagca gctacgtgtg gcggaacatc atccctcacg
     3181 tggcccctac acacagatgt atcgcccctg acctgatcgg catgggcaag agcgataagc
     3241 ccgacctggg ctacttcttc gacgaccacg tgcggttcat ggacgccttt attgaggccc
     3301 tcggcctgga agaggtggtg ctggttattc acgattgggg cagcgccctg ggattccact
     3361 gggccaagag aaaccccgag agagtgaagg gaatcgcctt catggaattc atcaggccca
     3421 ttcctacctg ggacgagtgg cctgagttcg ccagagagac attccaggcc ttcagaacca
     3481 ccgacgtggg cagaaagctg atcatcgacc agaacgtgtt catcgagggc accctgccta
     3541 tgggagtcgt cagaccactg accgaggtgg aaatggacca ctacagagag ccctttctga
     3601 accccgtgga ccgggaacct ctttggagat tccccaacga gctgcccatt gccggcgagc
     3661 ctgctaatat tgtggccctg gtggaagagt acatggactg gctgcatcag agccccgtgc
     3721 ctaagctgct gttttgggga acacccggcg tgctgattcc tcctgctgaa gctgccagac
     3781 tggccaagag cctgcctaat tgcaaggccg tggatatcgg ccctggcctg aatctgctgc
     3841 aagaggacaa ccccgatctg atcggctccg agattgccag atggctgagc accctggaaa
     3901 tcagcggagg aggaggttct ggaggcggtg gaagtggtgg cggaggtagc tctgaatata
     3961 ttcgggtaac cgaagatgag aacgatgagc ccattgaaat accatcggaa gacgatggga
     4021 cggtgctgct ctccacggtt acagcccagt ttccaggggc gtgtgggctt cgctacagga
     4081 atccagtgtc tcagtgtatg agaggtgtcc ggctggtaga aggaattctg catgccccag
     4141 atgctggctg gggaaatctg gtgtatgttg tcaactatcc aaaaggtttg ttaccatttg
     4201 gtttttgtaa tcatgctgaa gtgtgttcag gtgtgtgtct catccatgga tcttagcctc
     4261 ttttggtggc agaatgtctc ctgaaactta agtatttcct tgaacttcag tgttagacaa
     4321 gtttggaaga ccacgagtct aaatttatgg aagcttggcg taatcatggt cata
//
