LOCUS       sequence-349330-        4456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human HNRNPA2B1 at the 
            C-terminus with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4456)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HNRNPA2B1_Halo_C_allele
FEATURES             Location/Qualifiers
     source          1..4456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             711..1598
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     primer_bind     complement(2043..2060)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2043..2059)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2052..2074)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2268..2287)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2387..2409
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2447..2465)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2533..2637
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2638..2706,2707..3498)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2856..2875)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3669..4257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4158..4177
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4411..4428
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcagg cctctgcagt
      241 cgacgggccc gggatccgat ccaatattca aaccattgct tagcttattt ttgaggtttt
      301 tagctaggag taaactagct ttgtcttggg cttgatgtac ttttaaaaaa atcccttact
      361 cagtccaaat gaggatgaga gggtgaaagg accctttatt taaaagaata gggtcagcca
      421 cgaaataaaa atgtctatga acccgagtaa tttatctcct gagtaattct gctaactggc
      481 tgcaaaggat taggatctgc ttgtttaaaa gactggatgg atataaaata gaatcaactg
      541 tagtgttagg ctgatcatgg gaaatcaaag taagtttgtt ttctcttgct gttccaacaa
      601 ttataggaaa ctatggtcca ggaggcagtg gaggaagtgg gggttatggt gggaggagcc
      661 gatacggtgg aggaggttct ggaggcggtg gaagtggtgg cggaggtagc gccgagatcg
      721 gcaccggctt tcccttcgat cctcactacg tggaagtgct gggcgagaga atgcactatg
      781 tggacgtggg ccccagagat ggaacccctg tgctgtttct gcacggcaac cctaccagca
      841 gctacgtgtg gcggaacatc atccctcacg tggcccctac acacagatgt atcgcccctg
      901 acctgatcgg catgggcaag agcgataagc ccgacctggg ctacttcttc gacgaccacg
      961 tgcggttcat ggacgccttt attgaggccc tcggcctgga agaggtggtg ctggttattc
     1021 acgattgggg cagcgccctg ggattccact gggccaagag aaaccccgag agagtgaagg
     1081 gaatcgcctt catggaattc atcaggccca ttcctacctg ggacgagtgg cctgagttcg
     1141 ccagagagac attccaggcc ttcagaacca ccgacgtggg cagaaagctg atcatcgacc
     1201 agaacgtgtt catcgagggc accctgccta tgggagtcgt cagaccactg accgaggtgg
     1261 aaatggacca ctacagagag ccctttctga accccgtgga ccgggaacct ctttggagat
     1321 tccccaacga gctgcccatt gccggcgagc ctgctaatat tgtggccctg gtggaagagt
     1381 acatggactg gctgcatcag agccccgtgc ctaagctgct gttttgggga acacccggcg
     1441 tgctgattcc tcctgctgaa gctgccagac tggccaagag cctgcctaat tgcaaggccg
     1501 tggatatcgg ccctggcctg aatctgctgc aagaggacaa ccccgatctg atcggctccg
     1561 agattgccag atggctgagc accctggaaa tcagcggata agcttcttcc tatttgccat
     1621 gggtaagtag cttttgagtt ttacaattat tattatcttg ggagacatag ctgcaggagt
     1681 aaaagctttt taggatcatg ttatctttcc ttaaaatctg gttagatgga taatttcata
     1741 acctattttt tttttactct ttacttctgt tgaaacaggc ttcactgtat aaataggaga
     1801 ggatgagagc ccagaggtaa cagaacagct tcaggttatc gaaataacaa tgttaaggaa
     1861 actcttatct cagtcatgca taaatatgca gtgatatggc agaagacacc agagcagatg
     1921 cagagagcca ttttgtgaat ggattggatt atttaataac attaccttac tgtggaggaa
     1981 ggattgtaaa aaaaaatgcc tccaatatct agatgcattc gcgaggtacc gagctcgaat
     2041 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2101 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2161 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     2221 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     2281 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2341 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2401 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     2461 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2521 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2581 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2641 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2701 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2761 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2821 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2881 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2941 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3001 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3061 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3121 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3181 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3241 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3301 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggatctcgc
     3361 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3421 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3481 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3541 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3601 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3661 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3721 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3781 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3841 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3901 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3961 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4021 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4081 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4141 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4201 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4261 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4321 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4381 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4441 cgcccaatac gcaaac
//
