LOCUS       sequence-349334-       10027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3183
FEATURES             Location/Qualifiers
     source          1..10027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2781..2805)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2813..2854
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2965..3553
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3018..3038)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3436..3447)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3625..3858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3936..4057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3973..3992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4027..4046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4097..4232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4252..4271)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4253..4271)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4281..4298)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4281..4297)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4290..4312)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4439..4894
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4526..4545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4736..4757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4920..5024
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5025..5093,5094..5885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5243..5262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6056..6644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6545..6564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6798..6815
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6932..6953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6968..6973,6974..6991,6992..6998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7006..7022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7011..7033
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7030..7046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7030..7046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7065..7085
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7067..7085
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7113..7339
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7340..7520
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7567..7692
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8185..8418
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8603..8647
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8878..9088
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8900..9096
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8947..9082
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9128..9730
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9128..9147)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9624..9644
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9773..9890
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggcttc ctccccggcg gtggacgtgt cctgcaggcg
     1261 gcgggagaag cggcggcagc tggacgcgcg ccgcagcaag tgccgcatcc gcctgggcgg
     1321 ccacatggag cagtggtgcc tcctcaagga gcggctgggc ttctccctgc actcgcagct
     1381 cgccaagttc ctgttggacc ggtacacttc ttcaggctgt gtcctctgtg caggtcctga
     1441 gcctttgcct ccaaaaggtc tgcagtatct ggtgctcttg tctcatgccc acagccgaga
     1501 gtgcagcctg gtgcccgggc ttcgggggcc tggcggccaa gatggggggc ttgtgtggga
     1561 gtgctcagca ggccatacct tctcctgggg accctctttg agccctacac cttcagaggc
     1621 acccaagcca gcctcccttc cacatactac tcggagaagt tggtgttccg aggccacgag
     1681 tgggcaggag cttgcagatt tggaatctga gcatgatgag aggactcaag aggccaggtt
     1741 gcccaggagg gtgggacccc caccagagac cttcccacct ccaggagagg aagagggtga
     1801 ggaagaagag gacaatgatg aggatgaaga ggagatgctc agtgatgcca gcttatggac
     1861 ctacagctcc tccccagatg atagtgagcc tgatgccccc agactactgc cttcccctgt
     1921 cacctgcaca cctaaagagg gggagacacc accagcccct gcagcactct ccagtcctct
     1981 tgctgtgccg gccttgtcag catcctcatt gagttccaga gctcctccac ctgcagaagt
     2041 cagggtgcag ccacagctca gcaggacccc tcaagcggcc cagcagactg aggccctggc
     2101 cagcactggg agtcaggccc agtctgctcc aaccccggcc tgggatgagg acactgcaca
     2161 aattggcccc aagagaatta ggaaagctgc caaaagagag ctgatgcctt gtgacttccc
     2221 tggctgtgga aggatcttct ccaaccggca gtatttgaat caccacaaaa agtaccagca
     2281 catccaccag aagtctttct cctgcccaga gccagcctgt gggaagtctt tcaactttaa
     2341 gaaacacctg aaggagcaca tgaagctgca cagtgacacc cgggactaca tctgtgagtt
     2401 ctgcgcccgg tctttccgca ctagcagcaa ccttgtcatc cacagacgta tccacactgg
     2461 agaaaaaccc ctgcagtgtg agatatgcgg gtttacctgc cgccagaagg cttccctgaa
     2521 ctggcaccag cgcaagcatg cagagacggt ggctgccttg cgcttcccct gtgaattctg
     2581 cggcaagcgc tttgagaagc cagacagtgt tgcagcccac cgtagcaaaa gtcacccagc
     2641 cctgcttcta gcccctcaag agtcacccag tggtccccta gagccctgtc ccagcatctc
     2701 tgcccctggg cctctgggat ccagcgaggg gtccaggccc tctgcatctc ctcaggctcc
     2761 aaccctgctt cctcagcaat gcccaacttt cttgtacaaa gtggttgata tcggtaagcc
     2821 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga
     2881 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgacgata gcctgcctca
     2941 agtcaaatta cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     3001 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3061 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3121 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3181 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3241 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3301 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3361 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3421 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3481 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3541 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     3601 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     3661 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3721 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3781 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     3841 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     3901 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     3961 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4021 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     4081 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4141 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4201 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     4261 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4321 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4381 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     4441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4861 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     4921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6841 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6901 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6961 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7021 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     7081 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     7141 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     7201 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     7261 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     7321 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     7381 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7441 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7501 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     7561 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     7621 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     7681 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     7741 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     7801 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     7861 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     7921 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     7981 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     8041 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     8101 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     8161 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     8221 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     8281 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8341 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8401 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     8461 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     8521 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     8581 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     8641 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     8701 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     8761 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     8821 catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8881 ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact
     8941 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9001 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9061 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga
     9121 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     9181 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     9241 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     9301 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
     9361 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     9421 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     9481 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     9541 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     9601 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     9661 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     9721 tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     9781 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9841 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9901 acagggacag cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc aaagatggat
     9961 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
    10021 gggaatt
//
