LOCUS       sequence-34936-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  TITLE     Ning Zheng Lab Plasmids
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAL-hCul1-NTD
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgaagcaga ttggcctgga ccagatctgg
      121 gacgacctca gagccggcat ccagcaggtg tacacacggc agagcatggc caagtccaga
      181 tatatggagc tctacactca tgtttataac tactgtacta gtgttcacca gtcaaaccaa
      241 gcacgaggag ctggagttcc tccttctaag tcgaaaaagg ggcagacacc tggaggagct
      301 cagtttgttg gcctggaatt atataaacga cttaaggaat ttttgaagaa ttacttgaca
      361 aatcttctta aggatggaga agatttgatg gatgagagtg tactgaaatt ctacactcaa
      421 caatgggaag attatcgatt ttcaagcaaa gtgctgaatg gaatttgtgc ctacctcaat
      481 agacattggg ttcgccgtga atgtgacgaa ggacgaaaag gaatatatga aatctattcg
      541 cttgcattgg tgacttggag agactgtctg ttcaggccac tgaataaaca ggtaacaaat
      601 gctgttttaa agctgattga aaaggaaagg aatggtgaaa ccatcaatac aagattgatt
      661 agtggagttg tacagtctta cgtggaattg gggctgaatg aagatgatgc atttgcaaag
      721 ggccctacgt taacagtgta taaagaatcc tttgaatctc aatttttggc tgacacagag
      781 agattttata ccagagagag tactgaattc ttgcagcaga acccagttac tgaatatatg
      841 aaaaaggcag aggctcgtct gctngaggaa caacga
//
