LOCUS       sequence-349369-        4442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human HNRNPA1 at the 
            C-terminus with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4442)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HNRNPA1_Halo_C_allele
FEATURES             Location/Qualifiers
     source          1..4442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(132..149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(303..891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(383..402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1062..1853,1854..1922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1685..1704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1923..2027)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2095..2113
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2151..2173)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2273..2292
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2486..2508
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2500..2517
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2501..2517
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2998..3885
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     primer_bind     complement(4343..4359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4343..4359)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4356..4378)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4367..4383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4391..4397,4398..4415,4416..4421))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(4436..4442,1..15)
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
       61 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      121 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      181 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      241 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      301 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      361 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      421 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      481 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      541 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      601 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      661 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      721 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      781 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      841 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      901 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      961 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1021 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1081 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1141 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1201 cgagatccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1261 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1321 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1381 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1441 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1501 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1561 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1621 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1681 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1741 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1801 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1861 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1921 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1981 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2041 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2101 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2161 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2221 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2281 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2341 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2401 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2461 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     2521 ttcgagctcg gtacctcgcg aatgcatcta gagaagctct ggcccctatg gcggtggagg
     2581 ccaatacttt gcaaaaccac gaaaccaagg tatggtatct atgtaatttt ggataatgtc
     2641 aaaagagtgt ctgtagctac tgctgggaag aaagcccttt aactgctatg tctgggcagc
     2701 aaaacgttta tagtttagaa ccttcagaaa gtgataattt gatcacaaat tagaaaaatc
     2761 atgggacctc tttaccacct cccttgtagt agggccattt ttaaatggcc agacacttga
     2821 atttaacttt tattatccca aatatgaaaa cattactgtt ggcactttga aactttaaaa
     2881 gaaaaattgt acttttcagg tggctatggc ggttccagca gcagcagtag ctatggcagt
     2941 ggcagaagat ttggtggagg aggttctgga ggcggtggaa gtggtggcgg aggtagcgcc
     3001 gagatcggca ccggctttcc cttcgatcct cactacgtgg aagtgctggg cgagagaatg
     3061 cactatgtgg acgtgggccc cagagatgga acccctgtgc tgtttctgca cggcaaccct
     3121 accagcagct acgtgtggcg gaacatcatc cctcacgtgg cccctacaca cagatgtatc
     3181 gcccctgacc tgatcggcat gggcaagagc gataagcccg acctgggcta cttcttcgac
     3241 gaccacgtgc ggttcatgga cgcctttatt gaggccctcg gcctggaaga ggtggtgctg
     3301 gttattcacg attggggcag cgccctggga ttccactggg ccaagagaaa ccccgagaga
     3361 gtgaagggaa tcgccttcat ggaattcatc aggcccattc ctacctggga cgagtggcct
     3421 gagttcgcca gagagacatt ccaggccttc agaaccaccg acgtgggcag aaagctgatc
     3481 atcgaccaga acgtgttcat cgagggcacc ctgcctatgg gagtcgtcag accactgacc
     3541 gaggtggaaa tggaccacta cagagagccc tttctgaacc ccgtggaccg ggaacctctt
     3601 tggagattcc ccaacgagct gcccattgcc ggcgagcctg ctaatattgt ggccctggtg
     3661 gaagagtaca tggactggct gcatcagagc cccgtgccta agctgctgtt ttggggaaca
     3721 cccggcgtgc tgattcctcc tgctgaagct gccagactgg ccaagagcct gcctaattgc
     3781 aaggccgtgg atatcggccc tggcctgaat ctgctgcaag aggacaaccc cgatctgatc
     3841 ggctccgaga ttgccagatg gctgagcacc ctggaaatca gcggataatt aggtaagtaa
     3901 gcaccttttt gtgtgttgac ataatttttt aaattgctga tgaacccaat aaccctaatg
     3961 tagctgagca gtgcaacata gttaacatta taattgcagt aattgtggat ataaagttaa
     4021 tattcagatc agcaaaattt gtgggaaaca aacttgatat tggattgtag ccttgagtct
     4081 taatatgttt agattaacaa ctttattcca tattgttcaa caggaaacaa agcttagcag
     4141 gagaggagag ccagagaagt gacagggaag ctacaggtta caacagattt gtgaactcag
     4201 ccaagcacag tggtggcagg gcctagctgc tacaaagaag acatgtttta gacaaatact
     4261 catgtgtatg ggcaaaaaac tcgaggacgg atcccgggcc cgtcgactgc agaggcctgc
     4321 atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4381 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4441 tg
//
