LOCUS       sequence-34937-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     Ning Zheng Lab Plasmids
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCool-mRbx1-His-hCul1-CTP
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             368..390
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             409..426
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             436..453
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctggttcgcg tggatctcat atggatgtgg atacccccag cggcaccaac agcggcgcgg
       61 gcaagaagcg ctttgaagtt aaaaagtgga atgcagtggc cctctgggcc tgggacattg
      121 tggttgataa ctgtgccatc tgcaggaacc acattatgga tctttgtatc gaatgtcagg
      181 ccaaccaggc gtcagctact tccgaagagt gtacggttgc atggggagtc tgcaaccatg
      241 cttttcattt ccactgcatc tctcgatggc tcaaaacgag gcaggtgtgt ccgttggaca
      301 acagagagtg ggagttccag aagtatgggc attaactcga gcccggggga tcccctctag
      361 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat
      421 catcacagca gcggcctggt gccgcgcggc agccatatgg cccaatcatc cagtaaatcc
      481 cctgagttgc tggctcgata ctgtgactcc ttgttgaaga aaagttccaa gaacccagag
      541 gaggcagaac tagaagacac actcaatcaa gtgatggttg tcttcaagta catagaagac
      601 aaagacgtat ttcagaagtt ctatgcgaag atgctcgcca agaggctcgt ccaccagaac
      661 agtgcaagtg acgatgccga agccagcatg atctccaagt taaagcaagc ttgcgggttc
      721 gagtacacct ctaaacttca gcgcatgttt caagacattg gcgtgagcaa agatctgaac
      781 gagcaattca aaaagcactt gacaaactca gaacccctag acttggattt cagcattc
//
