LOCUS       sequence-34941-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: 426Gal-FUS-1-170aa-YFP
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(198..209)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(607..631)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(639..655)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(639..655)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(676..699)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc attaagcttg atatcgaatt cctgcagccc atcaccactt
       61 tgtacaagaa agctgggtct ccacctccac caccactgct gctgttgtac tggttctgct
      121 gtccatagcc ctgaggggga ttatagcttt gctgctgtcc atagctttgc tgctgtccac
      181 catagctagg ctgctggctg tagctcccac tctggggctg cccatagctg ctgctctgag
      241 aactgctacc gtaacttccc gaggtgctgc tgggagctgg ctgctggcca tagccaggat
      301 aggaggactg ctgcccgtaa gacgattggg agctctggct actgccatag ccgccagtcg
      361 agccatatcc ctggggagtt gactgagttc catagcctgt gttctggctc tggccataag
      421 aagaatagct gctctggcca tagcctgaag tgtccgtgga ctggctataa ccactgtaac
      481 tctgctgtcc gtagggctga ctgctctgct gggaatagcc ctgcccgggc tgggtggggt
      541 aggccccata gctttgggtt gcttgttggg tataatcgtt tgaggccatt ttgttatctc
      601 cttcgaagcc tgcttttttg tacaaacttg tgatggggga tccactagtt ctagaatccg
      661 gggttttttc tccttgacgt taaagtatag aggtatatta acaatttttt gttgatactt
      721 ttattacatt tgaataagaa gtaatacaaa ccgaaaatgt tgaaagtatt agttaaagtg
      781 gttatgcagt ttttgcattt atatatctgt taatagatca aaaatcatcg cttcgc
//
