LOCUS       sequence-349413-       19894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The insert is a codon optimized DNA sequence encoding VPS13D with an
            internal mScarlet protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19894)
  AUTHORS   Guillen-Samander A, Leonzino M, Hanna MG, Tang N, Shen H, De Camilli
            P
  TITLE     VPS13D bridges the ER to mitochondria and peroxisomes via Miro.
  JOURNAL   J Cell Biol. 2021 May 3;220(5). pii: 212021. doi: 
            10.1083/jcb.202010004.
  PUBMED    33891013
REFERENCE   2  (bases 1 to 19894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-05-3"; volume: "220(5). pii: 212021. doi"; 
            pages: " 10.1083/jcb.202010004"
COMMENT     Sequence Label: VPS13D^mScarlet
FEATURES             Location/Qualifiers
     source          1..19894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..30
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(149..169)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        154..483
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      334..469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     396..415
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             550..1344
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(604..623)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1214..1233
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1520..1641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1557..1576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1611..1630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1690..1706)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1690..1706)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1703..1725)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1714..1730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1738..1744,1745..1762,1763..1768))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1783..1804
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1921..1938)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2092..2680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2172..2191)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2851..3642,3643..3711))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3474..3493
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3712..3816)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3891..3910)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4082..4461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4462..4665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4616..4636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4662..4686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(9649..9667)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    18702..19290
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(18755..18775)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(19173..19184)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(19312..19331)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    19356..19404
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      join(19606..19894,1..140)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(19693..19712)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
       61 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta
      121 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      181 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      241 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      301 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      361 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      421 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      481 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
      541 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
      601 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
      661 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
      721 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
      781 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
      841 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
      901 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
      961 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1021 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1081 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1141 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1201 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1261 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1321 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc
     1381 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     1441 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1501 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     1561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1621 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     1681 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1741 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1801 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1861 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1921 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1981 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2041 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2101 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2161 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2221 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2281 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2341 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2401 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2461 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2521 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2581 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2641 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2701 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2761 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2821 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2881 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2941 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3001 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3061 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3121 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3181 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3241 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3301 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3361 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3421 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3481 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3541 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3601 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3661 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3721 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3781 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3841 tgacgtcgac ggatcgggag atctcccgat cccctatggt cgactctcag tacaatctgc
     3901 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     3961 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     4021 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     4081 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     4141 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4201 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4261 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4321 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4381 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4441 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4501 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4561 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     4621 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     4681 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     4741 tccagcctcc ggactctaga atgctggagg gcctggtcgc ttgggtcctg aacacctacc
     4801 tggggaagta tgtgaacaac ctgaacaccg atcagctgtc cgtcgctctg ctgaagggcg
     4861 ccgtggagct ggagaacctg ccactgaaga aggatgccct gaaggagctg gagctgccct
     4921 tcgaggtgaa ggccggcttt atcggcaagg tgaccctgca gatccctttt tacaggccac
     4981 acgtggaccc ctgggtcatc agcatcagct ccctgcacct gatcggcgcc cctgagaaga
     5041 tccaggactt caatgatgag aaggagaagc tgctggagag agagaggaag aaggccctgc
     5101 tgcaggccct ggaggagaag tggaagaacg accgccagca gaagggcgag tcctactggt
     5161 attctgtgac cgccagcgtg gtgacaagaa tcgtggagaa tatcgagctg aagatccagg
     5221 acgtgcacct gaggttcgag gatggcgtga ccaacccttc tcacccattc gcctttggca
     5281 tctgcatcaa gaatgtgagc atgcagaatg ccgtgaacga gcccgtgcag aagctgatgc
     5341 ggaagaagca gctggatgtg gccgagtttt ccatctactg ggacgtggat tgtacactgc
     5401 tgggcgacct gcctcagatg gagctgcagg aggcaatggc acgcagcatg gagagccggt
     5461 cccaccacta tgtgctggag ccagtgttcg ccagcgccct gctgaagcgc aactgctcca
     5521 agaagcctct gcgctctcgg cacagcccac ggatcgactg tgatatccag ctggagacaa
     5581 tccccctgaa gctgtcccag ctgcagtaca gacagatcat ggagtttctg aaggagctgg
     5641 agcgcaagga gcggcaggtg aagttccgga gatggaagcc taaggtggcc atcagcaaga
     5701 actgccgcga gtggtggtat ttcgccctga acgccaatct gtatgagatc cgggagcaga
     5761 gaaagaggtg tacctgggac tttatgctgc acagggcaag ggacgccgtg tcttacaccg
     5821 ataagtattt caataagctg aagggcggcc tgctgagcac agacgataag gaggagatgt
     5881 gccggatcga ggaggagcag tcctttgagg agctgaagat cctgcgcgag ctggtgcacg
     5941 atcggttcca caagcaggag gagctggccg agtctctgag agagccccag tttgacagcc
     6001 ctggcgcctg tccaggagca ccagagcctg gaggaggctc cggaatgctg cagtacctgc
     6061 agtcttggtt ccctggatgg ggaggatggt atggacagca gaccccagag ggaaacgtgg
     6121 tggagggcct gtccgccgag cagcaggagc agtggattcc cgaggagatc ctgggcaccg
     6181 aggagttctt tgacccaaca gccgatgcca gctgcatgaa cacatacacc aagcgcgacc
     6241 acgtgttcgc caagctgaat ctgcagctgc agcggggcac agtgaccctg ctgcacaagg
     6301 agcagggaac cccacagatg aacgagagcg ccttcatgca gctggagttt tccgatgtga
     6361 agctgctggc cgagtctctg cctaggcgca attctagcct gctgagcgtg cgcctgggag
     6421 gcctgtttct gcgggacctg gcaacagagg gcaccatgtt ccccctgctg gtgtttccaa
     6481 acccccagaa ggaagtgggc agagtgtctc agagcttcgg cctgcagacc acatccgccg
     6541 acaggtctga tcactaccca gcagccgacc ccgatggccc cgtgttcgag atgctgtatg
     6601 agagaaatcc cgcccacagc cacttcgagc ggagactgaa cgtgtccacc aggcccctga
     6661 atatcatcta caaccctcag gccatcaaga aggtggccga cttcttttac aagggcaagg
     6721 tgcacacatc tggcttcggc tatcagagcg agctggagct gagagtggca gaggcagcaa
     6781 ggcgccagta taacaagctg aagatgcaga ccaaggccga gatcagacag acactggaca
     6841 ggctgctggt gggcgacttc atcgaggagt ctaagcggtg gaccgtgcgg ctggatatca
     6901 gcgccccaca ggtcatcttc cccgacgatt tcaagtttaa gaatcctgtg ctggtggtgg
     6961 tggacctggg cagaatgctg ctgacaaata cccaggataa cagccggaga aagtccaggg
     7021 acggctccgc ctctgaggag acacagttct ccgacgatga gtacaagaca ccactggcca
     7081 ccccccctaa tacaccaccc cctgagtcct ctagctccaa cggcgagaag accccaccct
     7141 tctccggcgt ggagttttct gaggagcagc tgcaggccca cctgatgagc acaaagatgt
     7201 acgagcggta cagcctgtcc tttatggatc tgcagatcat ggtgggccgg gtgaaggaca
     7261 actggaagca cgtgcaggac atcgatgtgg gccccaccca cgtggtggag aagttcaatg
     7321 tgcacctgca gctggagagg cgcctgatct acacatccga cccaaagtat ccaggagccg
     7381 tgctgtctgg aaacctgcct gatctgaaga tccacatcaa tgaggacaag atcagcgccc
     7441 tgaagaactg ctttgccctg ctgaccacac cagagatgaa gacatccgat acccagatca
     7501 aggagaagat cttcccacag gaggagcaga ggggctctct gcaggacagc gtgatgaacc
     7561 tgacccagtc tatcgtgctg ctggagcagc acacaagaga ggtgctggtg gagagccagc
     7621 tgctgctggc cgagttcaag gtcaattgta tgcagctggg cgtggagtcc aacggcagat
     7681 acatctctgt gctgaaggtg ttcggcacca acgcccactt tgtgaagagg ccatatgatg
     7741 ccgaggtgag cctgacagtg cacggcctgc tgctggtgga cacaatgcag acctacggcg
     7801 ccgactttga tctgctgatg gccagccaca agaatctgtc cttcgatatc cccaccggct
     7861 ccctgagaga ctctagggca cagtcccccg tgtctggacc taatgtggca cacctgaccg
     7921 acggagccac actgaacgat aggtctgcca cctctgtgag cctggacaag atcctgacaa
     7981 aggagcagga gagcctgatc aagctggagt atcagttcgt gtctagcgag tgcccctcta
     8041 tgaacctgga tagcaccctg caggtcatca gcctgcaggt gaacaatctg gacatcatcc
     8101 tgaatcctga gacaatcgtg gagctgatcg gcttcctgca gaagtctttt ccaaaggaga
     8161 aggacgatct gagccctcag ccactgatga ccgatttcga gcggagcttc cgggagcagg
     8221 gcacctacca gtctacatat gagcagaata ccgaggtggc cgtggagatc cacagactga
     8281 acctgctgct gctgaggaca gtgggcatgg ccaatcgcga gaagtacgga aggaagatcg
     8341 caaccgcaag catcggcggc acaaaagtga atgtgagcat gggctccacc tttgacatga
     8401 acggctccct gggctgcctg cagctgatgg acctgacaca ggataatgtg aagaaccagt
     8461 atgtggtgag catcggcaat tccgtgggct acgagaacat catctctgat atcggctatt
     8521 tcgagagcgt gtttgtgcgc atggaggacg ccgccctgac cgaggccctg tccttcacat
     8581 ttgtggagcg gtctaagcag gagtgtttcc tgaatctgaa gatggccagc ctgcactaca
     8641 accactccgc caagtttctg aaggagctga ccctgtctat ggacgagctg gaggagaact
     8701 tcaggggcat gctgaagagc gccgccacaa aggtgaccac agtgctggcc acaaagaccg
     8761 ccgagtactc tgagatggtg agcctgttcg agacacctag aaagacaagg gagcccttca
     8821 tcctggagga gaatgagatc tatggcttcg acctggcctc ctctcacctg gataccgtga
     8881 agctgatcct gaacatcaat atcgagtccc ccgtggtgtc tatccctagg aagccaggct
     8941 cccccgagct gctggtggga cacctgggac agatcttcat ccagaacttt gtggccggcg
     9001 acgatgagtc ccgctctgat cggctgcagg tggagatcaa ggacatcaag ctgtacagcc
     9061 tgaattgcac ccagctggca ggaagagagg cagtgggctc tgagggcagc aggatgtttt
     9121 gtcctccaag cggctccggc tctgccaact cccaggagga ggcacacttc acacgccacg
     9181 acttctttga gtctctgcac cggggccagg cctttcacat cctgaacaat accacaatcc
     9241 agttcaagct ggagaagatc ccaatcgagc gggagagcga gctgaccttt agcctgtccc
     9301 ccgacgatct gggcacaagc tccatcatga agatcgaggg caagttcgtg aatccagtgc
     9361 aggtggtgct ggccaagcac gtgtacgagc aggtgctgca gaccctggac aacctggtgt
     9421 acagcgagga tctgaataag tatccagcat ccgccacatc tagcccatgc cctgattccc
     9481 cactgccagg ctctggctcc tctggatccg gaggaatggt gtccaaggga gaggccgtga
     9541 tcaaggagtt catgaggttt aaggtgcaca tggagggcag catgaacggc cacgagtttg
     9601 agatcgaggg agagggagag ggcagaccct acgagggcac acagaccgcc aagctgaagg
     9661 tgaccaaggg cggccctctg ccattctctt gggacatcct gagcccacag ttcatgtatg
     9721 gctctcgggc ctttatcaag cacccagccg acatccccga ttactataag cagagcttcc
     9781 ccgagggctt taagtgggag agagtgatga actttgagga tggaggagca gtgacagtga
     9841 cccaggacac ctccctggag gatggcacac tgatctacaa ggtgaagctg aggggaacca
     9901 atttcccacc tgacggcccc gtgatgcaga agaagaccat gggatgggag gcaagcacag
     9961 agcgcctgta tcctgaggac ggcgtgctga agggcgatat caagatggcc ctgagactga
    10021 aggacggcgg caggtacctg gccgatttca agaccacata taaggccaag aagcctgtgc
    10081 agatgccagg cgcctacaac gtggaccgga agctggatat caccagccac aatgaggatt
    10141 acacagtggt ggagcagtat gagagatccg agggcaggca ctctaccggc ggaatggacg
    10201 agctgggcag cggcagctcc ggatccggag gacccctgtc tacatgcggc gagtctagcg
    10261 tggagagaaa ggagaatggc ctgttttctc actcctctct gtccaacacc tctcagaagt
    10321 ctctgagcgt gaaggaggtg aagagcttca cccagatcca ggccacattt tgtatcagcg
    10381 agctgcaggt gcagctgtcc ggcgatctga ccctgggagc acagggcctg gtgtctctga
    10441 agttccagga ttttgaggtg gagttcagca aggaccaccc ccagacactg tctatccaga
    10501 tcgccctgca cagcctgctg atggaggacc tgctggagaa gaatcctgat agcaagtaca
    10561 agaacctgat ggtgtccagg ggagcaccaa agcctagctc cctggcacag aaggagtacc
    10621 tgtcccagtc ttgccctagc gtgtccaacg tggaatatcc tgacatgcca cgcagcctgc
    10681 catcccacat ggaggaggcc cccaacgtgt tccagctgta ccagcgccct acctctgcca
    10741 gccggaagaa gcagaaggag gtgcaggaca aggattatcc actgacccca ccccctagcc
    10801 ctacagtgga tgagccaaag atcctggtgg gcaagagcaa gtttgacgat tccctggtgc
    10861 acatcaacat cttcctggtg gacaagaagc acccagagtt ttctagctcc tacaatagag
    10921 tgaacaggtc catcgacgtg gatttcaatt gtctggacgt gctgatcaca ctgcagacct
    10981 gggtggtcat cctggacttc ttcggaatcg gcagcaccgc agacaaccac gccatgagac
    11041 tgccacccga gggcatcctg cacaatgtga agctggagcc ccacgcctct atggagagcg
    11101 gcctgcagga ccctgtgaac acaaagctgg atctgaaggt gcactccctg tctctggtgc
    11161 tgaataagac cacatctgag ctggccaagg ccaacgtgag caagctggtg gcccacctgg
    11221 agatgatcga gggcgatctg gccctgcagg gctccatcgg cagcctgtcc ctgtctgacc
    11281 tgacctgcca cggcgagttc tacagagaga ggtttaccac aagcggcgag gaggccctga
    11341 tcttccagac cttcaagtat ggccggccag atcccctgct gaggagagag cacgacatcc
    11401 gcgtgtctct gcggatggcc agcgtgcagt acgtgcacac ccagagattc caggccgagg
    11461 tggtggcctt catccagcac tttacacagc tgcaggatgt gctgggccgc cagagggcag
    11521 caatcgaggg acagaccgtg agggatcagg cacagcggtg ctcccgggtg ctgctggaca
    11581 tcgaggcagg agcccccgtg ctgctgatcc ctgagtctag ccgcagcaac aatctgatcg
    11641 tggccaacct gggcaagctg aaggtgaaga ataagttcct gtttgccggc ttccccggca
    11701 ccttttctct gcaggacaag gagagcgtgc caagcgcctc ccctacaggc atcccaaagc
    11761 actccctgag aaagaccaca tctaccgagg agccaagggg aacacactcc cagggccagt
    11821 ttaccatgcc tctggccggc atgtccctgg gctctctgaa gagcgagttc gtgccatcta
    11881 caagcaccaa gcagcagggc cctcagccaa cactgagcgt gggacaggag tcctctagcc
    11941 cagaggatca cgtgtgcctg ctggactgcg tggtggtgga tctgcaggac atggatatct
    12001 tcgccgccga gagacaccct agggagtaca gcaaggcccc agaggactcc tctggcgatc
    12061 tgatcttccc ctcttatttt gtgaggcaga ccggcggcag cctgctgaca gagccttgcc
    12121 gcctgaagct gcaggtggag cggaacctgg ataaggagat ctcccacacc gtgcctgaca
    12181 tctctatcca cggcaatctg agctccgtgc actgttccct ggacctgtac aagtataagc
    12241 tgatccgcgg cctgctggag aacaatctgg gcgagccaat cgaggagttc atgcggccct
    12301 acgacctgca ggaccccaga atccacaccg tgctgagcgg cgaggtgtat acatgcatgt
    12361 gcttcctgat cgatatggtg aacgtgagcc tggagctgaa ggaccccaag aggaaggagg
    12421 gagcaggctc cctggcacgg ttcgacttca agaagtgcaa gctgctgtac gagtcctttt
    12481 ctaatcagac caagtccatc aacctggtga gccactccat gatggccttc gacacacggt
    12541 atgccggcca gaagacctcc ccaggcatga caaacgtgtt ctcttgcatc tttcagcccg
    12601 ccaagaattc tagcaccaca cagggctcca tccagatcga gctgcacttc cgctctacca
    12661 aggactcctc ttgttttaca gtggtgctga acaatctgcg ggtgttcctg atctttgact
    12721 ggctgctgct ggtgcacgat ttcctgcaca ccccaagcga catcaagaag cagaatcacg
    12781 tgacacccag cagacacagg aacagctcct ctgagtccgc catcgtgcca aagaccgtga
    12841 agtctggcgt ggtgacaaag agaagctccc tgcccgtgtc caatgagagg cacctggagg
    12901 tgaaggtgaa cgtgacaggc accgagttcg tggtcatcga ggacgtgagc tgctttgata
    12961 ccaacgccat catcctgaag ggcaccacag tgctgacata caagccaaga ttcgtggaca
    13021 ggcccttttc tggcagcctg ttcggcatcg aggtgtttag ctgtagactg ggcaacgagc
    13081 acgataccgc cctgtccatc gtggaccctg tgcagatcca gatggagctg gtgggcaatt
    13141 ctagctacca gaactcctct ggcctgatgg acgccttcaa ttccgaggat tttcctccag
    13201 tgctggagat ccagctgcag gccctggata tcaggctgtc ctataacgac gtgcagctgt
    13261 tcctggccat cgccaagtct atccctgagc aggccaatgc agcagtgcca gatagcgtgg
    13321 ccctggagtc cgactctgtg ggcacctacc tgccaggagc atcccgcgtg ggagaggaga
    13381 tcagggaggg aacaaggcac accctggacc ccgtgctgga gctgcagctg gcaagactgc
    13441 aggagctggg cttttccatg gacgattgca ggaaggccct gctggcatgt cagggacagc
    13501 tgaagaaggc cgccagctgg ctgttcaaga acgcagagcc cctgaagtcc ctgtccctgg
    13561 catctaccag cagggacagc cctggagcag tggcagcacc actgatcagc ggcgtggaga
    13621 tcaaggccga gtccgtgtgc atctgtttca tcgacgattg catggactgt gatgtgcctc
    13681 tggccgagct gacattctcc aggctgaact ttctgcagcg cgtgcggacc tctccagagg
    13741 gatacgccca ctttacactg agcggcgact actataatcg cgccctgtcc ggctgggagc
    13801 cattcatcga gccctggcct tgctccgtgt cttggcagca gcaggcagca tccagactgc
    13861 acccacctag gctgaagctg gaggcaaagg caaagccacg cctggatatc aacatcacca
    13921 gcgtgctgat cgaccagtac gtgagcacaa aggagtcctg gatggccgat tattgcaagg
    13981 acgataagga catcgagtcc gccaagtctg aggattggat gggcagctcc gtggacccac
    14041 cctgtttcgg ccagagcctg cccctggtgt acctgagaac aaggagcacc gcctccctga
    14101 ccaacctgga gcaccagatc tatgcccgcg ccgaggtgaa gacacctaag aggcgccagc
    14161 ccttcgtgcc ttttgccctg cggaatcaca caggctgtac cctgtggttt gccaccctga
    14221 ccacaacccc aacaagagcc gccctgagcc actccggctc tcccggagtg gtgcctgagg
    14281 gaaatggcac cttcctggac gatacacaca acgtgtccga gtggagggag gtgctgaccg
    14341 gcgaggagat ccccttcgag tttgaggcaa ggggcaagct gaggcacagg cacacacacg
    14401 acctgagaat ccaccagctg caggtgaggg tgaacggctg ggagcaggtg tctcctgtga
    14461 gcgtggataa agtgggcacc ttctttcggt acgccgcccc agacaagaac tctagctcct
    14521 ctaccatcgg cagccccagc tccagaacaa atatcatcca ccctcaggtg tatttttcta
    14581 gcctgcctcc agtgagggtg gtgttcgccg tgaccatgga gggctctgcc agaaaagtga
    14641 tcacagtgag gagcgccctg atcgtgagaa accggctgga gacacccatg gagctgagac
    14701 tggacagccc atccgccccc gataagcctg tggtgctgcc agccatcatg cccggcgatt
    14761 cctttgccgt gcctctgcac ctgacatctt ggagactgca ggccaggcct aagggcctgg
    14821 gcgtgttctt ttgcaaggcc ccaatccact ggaccaatgt ggtgaagaca gccgagatct
    14881 cctctagcaa gagggagtgc cacagcatgg acactgagaa gtcccgcttc tttcggttct
    14941 gcgtggccat caagaaggag aactaccctg attatatgcc aagcaatatc ttctctgaca
    15001 gcgccaagca gatctttcgc cagcccggcc acaccatcta cctgctgcct acagtggtca
    15061 tctgcaacct gctgccctgt gagctggact tctacgtgaa gggcatgccc atcaatggca
    15121 ccctgaagcc tggcaaggag gccgccctgc acacagcaga cacctctcag aatatcgagc
    15181 tgggcgtgag cctggagaac ttccctctgt gcaaggagct gctgatcccc cctggcaccc
    15241 agaactacat ggtgcgcatg cggctgtatg acgtgaatcg gagacagctg aacctgacaa
    15301 tcaggatcgt gtgcagggca gagggctctc tgaagatctt catcagcgcc ccttactggc
    15361 tgatcaacaa gaccggcctg ccactgatct ttaggcagga caatgccaag acagatgcag
    15421 caggacagtt cgaggagcac gagctggcac gctccctgtc tcccctgctg ttttgctatg
    15481 ccgataagga gcagcctaac ctgtgcacca tgagaatcgg aaggggcatc cacccagagg
    15541 gaatgcctgg atggtgccag ggcttctccc tggacggagg ctctggcgtg cgggccctga
    15601 aagtgatcca gcagggcaat aggcctggcc tgatctacaa catcggcatc gatgtgaaga
    15661 agggcagagg caggtacatc gacacctgta tggtcatctt tgccccacgg tatctgctgg
    15721 acaacaagtc ctctcacaag ctggccttcg cccagagaga gtttgcaagg ggacagggaa
    15781 ccgcaaatcc agagggctac atcagcacac tgccaggcag ctccgtggtg ttccactggc
    15841 cccgcaacga ctatgatcag ctgctgtgcg tgcggctgat ggatgtgcct aattgtatct
    15901 ggagcggcgg cttcgaggtg aacaagaaca attcctttca catcaatatg agagacaccc
    15961 tgggcaagtg cttctttctg agggtggaga tcaccctgag gggagcaaca taccggatca
    16021 gcttttccga caccgatcag ctgccacccc ctttcaggat cgacaacttt tccaaggtgc
    16081 ctgtggtgtt cacacagcac ggagtggcag agcctaggct gaggaccgag gtgaagccaa
    16141 tgacaagcct ggactatgcc tgggatgagc caaccctgcc accctttatc acactgaccg
    16201 tgaagggcgc cggctctagc gagatcaact gcaatatgaa cgacttccag gataatcgcc
    16261 agctgtacta tgagaacttc atctacatcg ccgccacata taccttttcc ggcctgcagg
    16321 agggaaccgg ccggcccgtg gcctctaaca aggccatcac atgtgccgag ctggtgctgg
    16381 acgtgagccc taagacccag agagtgatcc tgaagaagaa ggagccaggc aagagatctc
    16441 agctgtggag gatgacaggc accggcatgc tggcacacga gggctcctct gtgccacaca
    16501 atcccaacaa gccttctgcc gcacggagca ccgagggctc cgccatcctg gatatcgcag
    16561 gcctggcagc agtgacagac aatcgctacg agcccctgat gctgaggaag cctgacaggc
    16621 gccggtccac aacccagacc tggtctttta gagagggcaa gctgacatgc ggcctgcacg
    16681 gcctggtggt gcaggcaaag ggaggcctgt ccggcctgtt cgatggagca gaggtggtgc
    16741 tgggaccaga cacctctatg gagctgctgg gccccgtgcc tccagagcag cagtttatca
    16801 accagaagat gcggccaggc tctggcatgc tgagcatccg cgtgatccca gatggaccaa
    16861 cccgggccct gcagatcaca gacttctgtc accgcaagag ctcccggtcc tatgaggtgg
    16921 atgagctgcc cgtgaccgag caggagctgc agaagctgaa gaatcctgac acagagcagg
    16981 agctggaggt gctggtgaga ctggagggcg gcattggcct gtccctgatc aacaaggtgc
    17041 ccgaggagct ggtgtttgcc tctctgaccg gcatcaatgt gcactacacc cagctggcca
    17101 caagccacat gctggagctg tccatccagg acgtgcaggt ggataaccag ctgatcggca
    17161 caacccagcc attcatgctg tatgtgaccc ccctgagcaa tgagaacgaa gtgatcgaga
    17221 caggcccagc agtgcaggtg aacgcagtga agtttccttc taagagcgcc ctgaccaata
    17281 tctacaagca cctgatgatc accgcccagc ggttcacagt gcagatcgag gagaagctgc
    17341 tgctgaagct gctgagcttc tttggctacg atcaggccga gtccgaggtg gagaagtatg
    17401 acgagaatct gcacgagaag accgcagagc agggaggaac acctatcaga tactattttg
    17461 agaacctgaa gatctccatc ccacagatca agctgtccgt gttcacctct aataagctgc
    17521 ccctggatct gaaggccctg aagagcacac tgggcttccc tctgatcaga tttgaggacg
    17581 ccgtgatcaa cctggacccc ttcaccaggg tgcaccccta cgagacaaag gagttcatca
    17641 tcaatgacat cctgaagcac tttcaggagg agctgctgtc ccaggccgcc agaatcctgg
    17701 gctctgtgga tttcctgggc aatcctatgg gcctgctgaa cgacgtgtcc gagggcgtga
    17761 ccggcctgat caagtatggc aatgtgggcg gcctgatccg gaacgtgaca cacggcgtgt
    17821 ccaattctgc cgcaaagttc gcaggcaccc tgagcgatgg cctgggcaag acaatggaca
    17881 acagacacca gagcgagaga gagtacatca ggtatcacgc agccacctcc ggagagcacc
    17941 tggtggccgg catccacggc ctggcacacg gaatcatcgg aggcctgacc agcgtgatca
    18001 catccaccgt ggagggagtg aagacagagg gaggcgtgtc tggctttatc agcggcctgg
    18061 gcaagggcct ggtgggaaca gtgaccaagc ccgtggcagg cgccctggat ttcgcaagcg
    18121 agacagcaca ggccgtgcgc gacacagcca ccctgtccgg accaaggacc caggcacaga
    18181 gagtgaggaa gccaaggtgc tgtacaggcc cacagggcct gctgccaagg tacagcgagt
    18241 cccaggcaga gggacaggag cagctgttca agctgaccga caacatccag gatgagttct
    18301 ttatcgccgt ggagaatatc gatagctatt gcgtgctgat ctctagcaag gccgtgtact
    18361 ttctgaaatc cggcgactat gtggatcggg aggccatctt cctggaggtg aagtacgacg
    18421 atctgtatca ctgtctggtg tccaaggacc acggcaaggt gtacgtgcag gtgaccaaga
    18481 aggccgtgtc tacatcctct ggcgtgagca tcccaggacc atcccaccag aagcccatgg
    18541 tgcacgtgaa gagcgaggtg ctggccgtga agctgtccca ggagatcaac tatgccaagt
    18601 ctctgtacta tgaacagcag ctgatgctga gactgagcga aaacagggaa cagctggaac
    18661 tggattcata aaccggttag taatgagttt gatatctcga caatcaacct ctggattaca
    18721 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
    18781 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
    18841 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
    18901 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
    18961 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
    19021 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
    19081 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
    19141 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
    19201 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
    19261 gtcggatctc cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg
    19321 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
    19381 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
    19441 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
    19501 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
    19561 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg
    19621 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    19681 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    19741 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc
    19801 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    19861 cggtttttcg ccctttgacg ttggagtcca cgtt
//
