LOCUS       sequence-349414-        9210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a GFP driven by human synapsin I promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Mattiassi S, Rizwan M, Grigsby CL, Zaw AM, Leong KW, Yim EKF
  TITLE     Enhanced efficiency of nonviral direct neuronal reprogramming on 
            topographical patterns.
  JOURNAL   Biomater Sci. 2021 Aug 7;9(15):5175-5191. doi: 10.1039/d1bm00400j. 
            Epub 2021 Jun 15.
  PUBMED    34128504
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d1bm00400j"; journalName: "Biomater Sci"; date: "2021-08-7-
            7"; volume: "9"; issue: "15"; pages: "5175-5191"
COMMENT     Sequence Label: pLV-hSyn1-GFP
FEATURES             Location/Qualifiers
     source          1..9210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(85..104)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        276..655
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        657..855
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     810..830
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             873..1053
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1100..1225
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1718..1951
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2136..2180
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2432..2548
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2582..3029
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3114..3123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3120..3818
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     misc_feature    4058..4646
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4111..4131)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4529..4540)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5053..5233
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5259..5276)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5265..5489
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5535..5963
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5622..5641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5832..5853
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5972..5992)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5977..6306
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6157..6292
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6219..6238
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6354..6401
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6420..6794
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6924..7045
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6961..6980)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7015..7034
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7094..7110)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7094..7110)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7107..7129)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7118..7134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7142..7148,7149..7166,7167..7172))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7187..7208
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7325..7342)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7496..8084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7576..7595)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8255..9046,9047..9115))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8878..8897
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9116..9210,1..10))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
       61 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
      121 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
      181 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
      241 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
      301 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      361 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      421 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      481 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      541 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      601 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      661 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      721 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      781 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      841 ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag
      901 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      961 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     1021 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     1081 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     1141 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     1201 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     1261 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     1321 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     1381 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1441 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1501 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1561 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1621 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1681 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1741 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1801 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1861 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1921 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1981 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     2041 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     2101 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     2161 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     2221 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     2281 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     2341 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     2401 acagatccat tcgattagtg aacggatcaa cttttaaaag aaaagggggg attggggggt
     2461 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2521 aaaaacaaat tacaaaattc aaaattttat cgatgctaga ctgcagaggg ccctgcgtat
     2581 gagtgcaagt gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg
     2641 accccgaccc actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag
     2701 agagggggag gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc
     2761 ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg
     2821 cgctgacgtc actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc
     2881 gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc
     2941 gcgaccatct gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg
     3001 gaggagtcgt gtcgtgcctg agagcgcagt cgatctagac gataagggcg aattctgcag
     3061 atatccatca cactggcggc cgctcgagca tgcatctaga ggatccaccg gtcgccacca
     3121 tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc
     3181 tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccgag cagggccgca
     3241 tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc
     3301 acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct
     3361 tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg
     3421 gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact
     3481 tcaaggtgat gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc
     3541 gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgatctg gatggcagct
     3601 tcacccgcac cttcagcctg cgcgacggcg gctactacag ctccgtggtg gacagccaca
     3661 tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct
     3721 tccgccgcgt ggaggaggat cacagcaaca ccgagctggg catcgtggag taccagcacg
     3781 ccttcaagac cccggatgca gatgccggtg aagaataaag cggccgcgac tctagatcat
     3841 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     3901 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aaacctggaa aaacatggag
     3961 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     4021 aggaggagga ggtgggtttt ccagtcacac ctcagacaat caacctctgg attacaaaat
     4081 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4141 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4201 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4261 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4321 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4381 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4441 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     4501 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4561 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4621 gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg
     4681 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     4741 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     4801 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     4861 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     4921 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     4981 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     5041 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5101 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5161 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5221 aaaatctcta gcagggccgc tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     5281 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     5341 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     5401 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     5461 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     5521 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5581 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5641 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5701 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5761 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5821 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5881 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5941 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     6001 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6061 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6121 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6181 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6241 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     6301 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     6361 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     6421 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     6481 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     6541 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     6601 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     6661 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     6721 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     6781 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     6841 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6901 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     6961 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7021 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     7081 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7141 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7201 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7261 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7321 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7381 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7441 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7501 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7561 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7621 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7681 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7741 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7801 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7861 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7921 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7981 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8041 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8101 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8161 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8221 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8281 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8341 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8401 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8461 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8521 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8581 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8641 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8701 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8761 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8821 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8881 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8941 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9001 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9061 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9121 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9181 ttgaatgtat ttagaaaaat aaacaaatag
//
