LOCUS       sequence-34942-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: 416Gal-SV40NLS-FUS-YFP
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..35
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             59..79
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             457..468
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atcccccatc acaagtttgt acaaaaaagc aggcttcgaa ggagataaca aaatgccacc
       61 aaaaaaaaaa agaaaagttg cctcaaacga ttatacccaa caagcaaccc aaagctatgg
      121 ggcctacccc acccagcccg ggcagggcta ttcccagcag agcagtcagc cctacggaca
      181 gcagagttac agtggttata gccagtccac ggacacttca ggctatggcc agagcagcta
      241 ttcttcttat ggccagagcc agaacacagg ctatggaact cagtcaactc cccagggata
      301 tggctcgact ggcggctatg gcagtagcca gagctcccaa tcgtcttacg ggcagcagtc
      361 ctcctatcct ggctatggcc agcagccagc tcccagcagc acctcgggaa gttacggtag
      421 cagttctcag agcagcagct atgggcagcc ccagagtggg agctacagcc agcagcctag
      481 ctatggtgga cagcagcaaa gctatggaca gcagcaaagc tataatcccc ctcagggcta
      541 tggacagcag aaccagtaca acagcagcag tggtggtgga ggtggaggtg gaggtggagg
      601 taactatggc caagatcaat cctccatgag tagtggtggt ggcagtggtg gcggttatgg
      661 caatcaagac cagagtggtg gaggtggcag cggtggctat ggacagcagg accgtggagg
      721 ccgcggcagg ggtggcagtg gtggcggcgg cggcggcggc ggtggtggtt acaaccgcag
      781 cagtggtggc tatgaaccca gaggtcgtgg aggtggccgt ggaggcagag gtggcatggg
      841 cggaagtgac cgtggtggct tcaataaatt tggtggccct cggggaccaa ggatcacgtc
      901 atgactccga acaggataat tcagacaaca acaccatctt tgtgc
//
