LOCUS       sequence-349433-        9763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding TRAPPC10 and EmGFP-Bsd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: TRAPPC10_pcDNA6.2/EmGFP-Bsd
FEATURES             Location/Qualifiers
     source          1..9763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    297..321
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4105..4129)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4155..4196
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4236..4255)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4280..4328
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4530..4958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4617..4636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4827..4848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4973..5472
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5008..5031)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5405..5424
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        5486..5533
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(5552..5554,5555..5557,5558..6268)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5597..5618)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5858..5877)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6205..6226
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6269..6664
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6822..6943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6859..6878)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6913..6932
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6992..7008)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6992..7008)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7005..7027)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7016..7032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7040..7046,7047..7064,7065..7070))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7085..7106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7223..7240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7394..7982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7474..7493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8153..8944,8945..9013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8776..8795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9014..9118)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9193..9212)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9384..9763
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag ctatcaacaa
      301 gtttgtacaa aaaagcaggc accatggacg cctctgagga gccgctgccg ccggtgatct
      361 acaccatgga gaacaagccc atcgtcacct gtgctggaga tcagaattta tttacctctg
      421 tttatccaac gctctctcag cagcttccaa gagaaccaat ggaatggaga aggtcctatg
      481 gccgggctcc gaagatgatt cacctagagt ctaactttgt tcaattcaaa gaggagctgc
      541 tgcccaaaga aggaaacaaa gctctgctca cgtttccctt cctccatatt tactggacag
      601 agtgctgtga taccgaagtg tataaagcta cagtaaaaga tgacctcacc aagtggcaga
      661 atgttctgaa ggctcatagc tctgtggact ggttaatagt gatagttgaa aatgatgcca
      721 agaaaaaaaa caaaaccaac atccttcccc gaacctctat tgtggacaaa ataagaaatg
      781 atttttgtaa taaacagagt gacaggtgtg ttgtgctctc cgaccccttg aaggactctt
      841 ctcgaactca ggaatcctgg aatgccttcc tgaccaaact caggacattg cttcttatgt
      901 cttttaccaa aaacctaggc aagtttgagg atgacatgag aaccttgagg gagaagagga
      961 ctgagccagg ctggagcttt tgtgaatatt tcatggttca ggaggagctt gcctttgttt
     1021 tcgagatgct gcagcagttc gaggacgccc tggtgcagta cgacgaactg gacgccctct
     1081 tctctcagta tgtggtcaac ttcggggccg gggatggtgc caactggctg acttttttct
     1141 gccagccagt gaagagctgg aacggattga tcctccgaaa acccatagat atggagaagc
     1201 gggaatcgat ccagaggcga gaagccaccc tgttagatct gcgcagttac ctgttctctc
     1261 gccagtgcac cttgctgctc ttcctgcaga ggccgtggga ggtggcccag cgcgccctag
     1321 agctgctgca caactgcgtg caggaactga agctcttaga agtctctgtc ccacctggtg
     1381 ctctggactg ctgggtgttt ctgagctgtc tggaggtgtt gcagaggata gaaggctgct
     1441 gtgaccgggc acagatcgac tcaaacattg cccacactgt ggggctatgg agctatgcca
     1501 cagaaaagtt aaagtccttg ggctatctat gtggacttgt gtcagagaaa ggacctaact
     1561 cagaagatct caacaggaca gttgaccttt tggcaggttt gggagctgag cgaccagaaa
     1621 cagccaacac agctcagagt ccttataaga aactgaaaga agcattatcg tcagtggaag
     1681 cttttgaaaa acactactta gatttgtccc atgccaccat tgaaatgtat acaagcattg
     1741 ggaggattcg atctgctaag tttgttggaa aagatctggc agagttttac atgaggaaaa
     1801 aggctccaca aaaggcagaa atctatcttc aaggagcact gaaaaactac ctggctgagg
     1861 gctgggcact ccccatcaca cacacaagga agcagctggc cgaatgtcaa aagcaccttg
     1921 gacaaattga aaactacctg cagaccagca gcctcttagc cagtgaccac cacctcactg
     1981 aagaggagcg caagcacttc tgccaggaga tacttgactt tgccagccag ccgtcagaca
     2041 gcccaggtca taagatagtg ctacccatgc attcctttgc acaactgcga gatctccatt
     2101 ttgatccctc caatgccgtg gtccacgtgg gcggcgtttt gtgcgttgag ataaccatgt
     2161 acagccagat gcctgtgcct gttcacgtgg agcagattgt ggtcaatgtc cacttcagca
     2221 ttgagaaaaa cagctaccgg aagactgcgg agtggcttac caagcacaag acgtccaatg
     2281 ggatcattaa ctttccaccc gagaccgcac ctttccctgt atcccaaaac agtttgcccg
     2341 cgctggagtt gtatgaaatg tttgagagaa gcccatctga taactccttg aacacgactg
     2401 ggattatctg cagaaacgtc cacatgctcc tgagaaggca ggagagcagc tcctctctag
     2461 agatgccctc aggggtggct ctggaggagg gtgcccacgt gctgaggtgc agccacgtga
     2521 ccctggaacc aggggccaac cagataacat tcaggactca ggccaaggaa cctggaacgt
     2581 atacactcag gcagctgtgc gcctcggtgg gctccgtgtg gttcgtcctc cctcacatct
     2641 accccattgt gcagtacgac gtgtactcac aggagcccca gctgcacgtg gagccgctgg
     2701 ctgatagcct tctggcaggc attcctcaga gagtcaagtt cactgtcact accggccatt
     2761 atacgataaa gaatggagac agcctgcagc ttagcaatgc cgaagccatg ctcatcctgt
     2821 gccaggcgga gagcagggct gtggtctact ccaacacgag agaacagtct tctgaggccg
     2881 cgctccggat tcagtcctcc gacaaggtca cgagcatcag tctgcctgtt gcgcctgcgt
     2941 accacgtgat cgaatttgaa ctggaagttc tctctttacc ttcagcccca gcactcggag
     3001 gggagagtga catgctgggg atggcagagc cccacaggaa gcataaggac aaacagagaa
     3061 ctggccgctg catggttacc acagaccaca aagtgtcgat tgactgcccg tggtccatct
     3121 actccacagt catcgcactg accttcagcg tacccttcag gaccacacac agcctcctgt
     3181 cctcaggaac acggaaatat gttcaagttt gtgtccagaa tttgtcagaa cttgactttc
     3241 agctgtcaga tagttatctt gtagataccg gtgatagtac cgacctgcaa ctagtaccac
     3301 tgaacacgca gtcccagcag cccatctaca gcaagcagtc ggtgttcttc gtctgggaac
     3361 tcaagtggac agaagagcct cccccttctc tgcattgccg gttctctgtt ggattttccc
     3421 cagcttctga ggaacagctg tctatctcct taaagccgta tacttatgaa tttaaagtgg
     3481 aaaatttttt tacattatac aacgtgaagg ctgagatctt tcccccttcg ggaatggagt
     3541 attgcagaac aggctccctc tgctccctgg aggttttgat cacgaggctc tcagacctct
     3601 tggaggtgga taaagatgaa gcactgactg aatctgatga gcatttttcg acaaagctta
     3661 tgtatgaagt tgtcgacaac agtagcaact gggcagtgtg tgggaaaagc tgcggtgtca
     3721 tctccatgcc agtggctgct cgggccactc acagggtcca catggaagtg atgccgctct
     3781 tcgccgggta tctccccctg cccgacgtca ggctgttcaa gtacctcccc catcattctg
     3841 cacactcctc ccaactggac gctgacagct ggatagaaaa cgacagcctg tcagtagaca
     3901 agcacgggga cgaccagccg gacagcagca gcctcaagag caggggcagc gtgcattcgg
     3961 cctgcagcag cgagcacaaa ggcctaccca tgccccggct gcaggcactg ccggccggcc
     4021 aggtcttcaa ctccagctcg ggcacacaag tcctggtcat ccccagccaa gatgaccacg
     4081 tcctggaagt cagtgtaaca tagaacccag ctttcttgta caaagtggtt gatctagagg
     4141 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     4201 ccggttagta atgagtttaa acgggggagg ctaactgaaa cacggaagga gacaataccg
     4261 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc
     4321 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     4381 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     4441 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatct
     4501 gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4561 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4621 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4681 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4741 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4801 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4861 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4921 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattat cctcgagcta ccgggtaggg
     4981 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     5041 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     5101 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     5161 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     5221 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     5281 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     5341 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     5401 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     5461 ccgggccttt cgactctaga cacgtgttga caattaatca tcggcatagt atatcggcat
     5521 agtataatac gacaaggtga ggaactaaac catggtgagc aagggcgagg agctgttcac
     5581 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     5641 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     5701 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcacct acggcgtgca
     5761 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5821 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5881 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     5941 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     6001 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca
     6061 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     6121 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     6181 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     6241 cactctcggc atggacgagc tgtacaaggc caagcctttg tctcaagaag aatccaccct
     6301 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     6361 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     6421 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     6481 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     6541 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     6601 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     6661 ctaagcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     6721 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6781 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6841 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6901 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6961 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7021 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7081 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7141 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7201 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7261 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7321 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7381 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7441 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7501 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7561 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7621 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7681 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7741 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7801 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7861 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7921 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7981 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8041 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8101 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8161 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8221 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8281 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8341 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8401 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8461 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagcttcg
     8521 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8581 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8641 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8701 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8761 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8821 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8881 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8941 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9001 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9061 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9121 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     9181 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     9241 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     9301 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     9361 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     9421 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     9481 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     9541 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     9601 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     9661 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     9721 tacttggcag tacatctacg tattagtcat cgctattacc atg
//
