LOCUS       sequence-34944-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: 416GPD-FUS-YFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     75..91
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     75..91
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    99..123
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             509..520
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tttacttctt aattctactt ttatagttag tctttttttt agttttaaaa caccagaact
       61 tagtttcgac ggattctaga actagtggat cccccatcac aagtttgtac aaaaaagcag
      121 gctccaccat ggcctcaaac gattataccc aacaagcaac ccaaagctat ggggcctacc
      181 ccacccagcc cgggcagggc tattcccagc agagcagtca gccctacgga cagcagagtt
      241 acagtggtta tagccagtcc acggacactt caggctatgg ccagagcagc tattcttctt
      301 atggccagag ccagaacaca ggctatggaa ctcagtcaac tccccaggga tatggctcga
      361 ctggcggcta tggcagtagc cagagctccc aatcgtctta cgggcagcag tcctcctatc
      421 ctggctatgg ccagcagcca gctcccagca gcacctcggg aagttacggt agcagttctc
      481 agagcagcag ctatgggcag ccccagagtg ggagctacag ccagcagcct agctatggtg
      541 gacagcagca aagctatgga cagcagcaaa gctataatcc ccctcagggc tatggacagc
      601 agaaccagta caacagcagc agtggtggtg gaggtggagg tggaggtgga ggtaactatg
      661 gccaagatca atcctccatg agtagtggtg gtggcagtgg tggcggttat ggcaatcaag
      721 accagagtgg tggaggtggc agcggtggct atggacagca ggaccgtgga ggccgcggca
      781 ggggtggcag tggtggcggc ggcggcggcg gcggtggtgg ttacaaccgc agcagtggtg
      841 gctatgaacc cagaggtcgt
//
