LOCUS       sequence-349449-        9401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Ohno H, Hirabayashi S, Iizuka T, Ohnishi H, Fujita T, Hata Y
  TITLE     Localization of p0071-interacting proteins, plakophilin-related 
            armadillo-repeat protein-interacting protein (PAPIN) and ERBIN, in 
            epithelial cells.
  JOURNAL   Oncogene. 2002 Oct 10;21(46):7042-9.
  PUBMED    12370826
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene.";
            date: "2002-10-10"; volume: "21(46)"; pages: "7042-9"
COMMENT     Sequence Label: pClneoMyc human ERBIN
FEATURES             Location/Qualifiers
     source          1..9401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     543..563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     589..613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          753..885
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     868..887
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        930..948
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             966..995
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(4448..4459)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4968..5089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4979..4998)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5033..5052
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5275..5730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5362..5381)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5572..5593
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5847..6204
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5847..6038
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5870..5890)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6055..6190
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6255..7049
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6309..6328)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6919..6938
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7113..7161
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7202..7221)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7321..7343
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7381..7399)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7467..7571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7572..7640,7641..8432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7790..7809)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8603..9191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9092..9111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctgcgatcgc ccgccccgtt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      601 gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa ttgctaacgc
      661 agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agtgactctc ttaaggtagc
      721 cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt
      781 aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg
      841 cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc cactcccagt
      901 tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct agacccccca
      961 acatggagca gaagcttatc agcgaggagg acctgctcga ggaattcacg cgtatgacta
     1021 caaaacgaag tttgtttgtg cggttggtac catgtcgctg tctacgaggg gaagaggaga
     1081 ctgtcactac tcttgattat tctcattgca gcttagaaca agttccgaaa gagattttta
     1141 cttttgaaaa aaccttggag gaactctatt tagatgctaa tcagattgaa gagcttccaa
     1201 agcaactttt taactgtcag tctttacaca aactgagttt gccagacaat gatttaacaa
     1261 cgttaccagc atccattgca aaccttatta atctcaggga actggatgtc agcaagaatg
     1321 gaatacagga gtttccagaa aatataaaaa attgtaaagt tttgacaatt gtggaggcca
     1381 gtgtaaaccc tatttccaag ctccctgatg gattttctca gctgttaaac ctaacccagt
     1441 cgtatctgaa tgatgctttt cttgagttct tgccagcaaa ttttggcaga ttaactaaac
     1501 tccaaatatt agagcttaga gaaaaccagt taaaaatgtt gcctaaaact atgaatagac
     1561 tgacccagct ggaaagattg gatttgggaa gtaacgaatt cacggaagtg cctgaagtac
     1621 ttgagcaact aagtggattg aaagagtttt ggatggatgc taatagactg acttttattc
     1681 cagggtttat tggtagtttg aaacagctca catatttgga tgtttctaaa aataatattg
     1741 aaatggttga agaaggaatt tcaacatgtg aaaaccttca agacctccta ttatcaagca
     1801 attcacttca gcagcttcct gagactattg gtttgttgaa gaatataaca acgcttaaaa
     1861 tagatgaaaa ccagttaatg tatctgccag actctatagg agggttaata tcagtagaag
     1921 aactggattg tagtttcaat gaagttgaag ctttgccttc atctattggg cagcttacta
     1981 acttaagaac ttttgctgct gatcataatt acttacagca gttgctccca gagattggaa
     2041 gctggaaaaa tataactgtg ctgtttctcc attccaataa acttgagaca cttccagagg
     2101 aaatgggtga tatgcaaaaa ttaaaagtca ttaatttaag tgataataga ttaaagaatt
     2161 taccctttag ctttacaaag ctacagcaat tgacagctat gtggctctca gataatcagt
     2221 ccaaacccct gatacctctt caaaaagaaa ctgattcaga gacccagaaa atggtgctta
     2281 ccaactacat gttccctcaa cagccaagga ctgaggatgt tatgtttata tcagataatg
     2341 aaagttttaa cccttcattg tgggaggaac agaggaaaca gcgggctcaa gttgcatttg
     2401 aatgtgatga agacaaagat gaaagggagg cacctcccag ggagggaaat ttaaaaagat
     2461 atccaacacc atacccagat gagcttaaga atatggtcaa aactgttcaa accattgtac
     2521 atagattaaa agatgaagag accaatgaag actcaggaag agatttgaaa ccacatgaag
     2581 atcaacaaga tataaataaa gatgtgggtg tgaagacctc agaaagtact actacagtaa
     2641 aaagcaaagt tgatgaaaga gaaaaatata tgataggaaa ctctgtacag aagatcagtg
     2701 aacctgaagc tgagattagt cctgggagtt taccagtgac tgcaaatatg aaagcctctg
     2761 agaacttgaa gcatattgtt aaccatgatg atgtttttga ggaatctgaa gaactttctt
     2821 ctgatgaaga gatgaaaatg gcggagatgc gaccaccatt aattgaaacc tctattaacc
     2881 agccaaaagt cgtagcactt agtaataaca aaaaagatga tacaaaggaa acagattctt
     2941 tatcagatga agttacacac aatagcaatc agaataacag caattgttct tctccatctc
     3001 ggatgtctga ttcagtttct cttaatactg atagtagtca agacacctca ctctgctctc
     3061 cagtgaaaca aactcatatt gatattaatt ccaaaatcag gcaagaagat gaaaatttta
     3121 acagcctttt acaaaatgga gatattttaa acagttcaac agaggaaaag ttcaaagctc
     3181 atgataaaaa agattttaac ttacctgaat atgatttgaa tgttgaagag cgattagttc
     3241 taattgagaa aagtgttgac tcaacagcca cagctgatga cactcacaaa ttagatcata
     3301 tcaatatgaa tcttaataaa cttataacta atgatacatt tcaaccagag atcatggaaa
     3361 gatcaaaaac acaggatatt gtgcttggaa caagcttttt aagcattaat tctaaagagg
     3421 aaactgagca cttggaaaat ggaaacaagt atcctaattt ggaatccgta aataaggtaa
     3481 atggacattc tgaggaaact tcccagtctc ctaataggac tgaaccacat gacagtgatt
     3541 gttctgttga cttaggtatt tccaaaagca ctgaagatct ctcccctcag aaaagtggtc
     3601 cagttggatc tgttgtgaaa tctcatagca taactaatat ggagattgga gggctaaaaa
     3661 tctatgatat tcttagtgat aatggacctc agcagccaag tacaaccgtt aaaatcacat
     3721 ctgctgttga tggaaaaaat atagtcagga gcaagtctgc cacactgttg tatgatcaac
     3781 cattgcaggt atttactggt tcttcctcat cttctgattt aatatcagga acaaaggcaa
     3841 ttttcaagtt tgattcaaat cataatcccg aagagccaaa tataataaga ggccccacaa
     3901 gtggcccaca atctgcacct caaatatatg gtcctccaca gtataatatc caatacagta
     3961 gcagtgctgc agtcaaagac actttgtggc actccaaaca aaatccccaa atagaccatg
     4021 ccagttttcc tcctcagctc cttcctagat cagagagcac agaaaatcaa agttatgcta
     4081 aacattctgc caatatgaat ttctctaatc ataacaatgt tcgagctaat actgcatacc
     4141 atttacatca gagacttggc ccagcaagac atggggaaat gtgggccatc tcaccaaacg
     4201 accgacttat tcctgcagta actcgaagta caatccagcg acaaagtagt gtgtcctcca
     4261 cagcctctgt aaatcttggt gatccaggct ctacaaggcg ggctcagatt cctgaaggag
     4321 attatttatc atacagagag ttccactcag cgggaagaac tcctccaatg atgccaggat
     4381 cacagagacc cctttctgca cgaacataca gcatagatgg tccaaatgca tcaagacctc
     4441 agagtgctcg accctctatt aatgaaatac cagagagaac tatgtcagtt agtgatttca
     4501 attattcacg gactagtcct tcaaaaagac caaatgcaag ggttggttct gagcattctt
     4561 tattagatcc tccaggaaaa agtaaagttc ctcgtgactg gagagaacaa gtacttcgac
     4621 atattgaagc caaaaagtta gaaaagattc gagtgagggt tgaaaaggat ccagaacttg
     4681 gatttagcat atcaggtggt gtcgggggta gaggaaaccc attcagacct gatgatgatg
     4741 gtatatttgt aacaagggta caacctgaag gaccagcatc aaaattactg cagccaggtg
     4801 ataaaattat tcaggctaat ggctacagtt ttataaatat tgaacatgga caagcagtgt
     4861 ccttgctaaa aactttccag aatacagttg aactcatcat tgtacgagaa gtttcctcat
     4921 agcggccgct tccctttagt gagggttaat gcttcgagca gacatgataa gatacattga
     4981 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     5041 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     5101 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta
     5161 aaacctctac aaatgtggta aaatccgata aggatcgatc cgggctggcg taatagcgaa
     5221 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
     5281 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5341 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5401 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5461 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5521 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5581 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5641 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5701 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     5761 gtgcggtatt tcacaccgca tacgcggatc tgcgcagcac catggcctga aataacctct
     5821 gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt
     5881 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5941 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6001 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6061 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6121 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6181 tttggaggcc taggcttttg caaaaagctt gattcttctg acacaacagt ctcgaactta
     6241 aggctagagc caccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     6301 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     6361 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     6421 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     6481 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     6541 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     6601 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     6661 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     6721 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     6781 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     6841 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     6901 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     6961 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     7021 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     7081 agcgacgccc aacctgccat cacgatggcc gcaataaaat atctttattt tcattacatc
     7141 tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat ccgcgtatgg tgcactctca
     7201 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     7261 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     7321 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     7381 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7441 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7501 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7561 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7621 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7681 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7741 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7801 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7861 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7921 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7981 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8041 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8101 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8161 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8221 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8281 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8341 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8401 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8461 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8521 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8581 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8641 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8701 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8761 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8821 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8881 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8941 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9001 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9061 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9121 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9181 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9241 ttgctcacat ggctcgacag atcttcaata ttggccatta gccatattat tcattggtta
     9301 tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat
     9361 gtacatttat attggctcat gtccaatatg accgccatgt t
//
