LOCUS       sequence-349452-        6156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 1, position 2, transcriptional unit for transient 
            expression of strictosidine synthase (CrSTR) from Catharanthus 
            roseus driven by 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD1CB0775
FEATURES             Location/Qualifiers
     source          1..6156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    149..173
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        359..704
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     647..667
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    1888..2062
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2107..2131
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2287..3435)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(3771..4359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3851..3870)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4530..5321,5322..5390))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5153..5172
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5391..5495)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(5582..6156,1..57)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgca
       61 aaggagatcc tgatctgact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
      121 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
      181 cgcttagaca acttaataac acattgcgga cgtttttaat gtactggggt ggatgcagtg
      241 ggccccactc tgtgaagaca agcaagaatt caagcttgga ggtcaacatg gtggagcacg
      301 acactctggt ctactccaaa aatgtcaaag atacagtctc agaagatcaa agggctattg
      361 agacttttca acaaaggata atttcgggaa acctcctcgg attccattgc ccagctatct
      421 gtcacttcat cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg
      481 ataaaggaaa ggctatcatt caagatctct ctgccgacag tggtcccaaa gatggacccc
      541 cacccacgag gagcatcgtg gaaaaagaag aggttccaac cacgtctaca aagcaagtgg
      601 attgatgtga catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
      661 acccttcctc tatataagga agttcatttc atttggagag gacacgctcg agtataagag
      721 ctcattttta caacaattac caacaacaac aaacaacaaa caacattaca attacattta
      781 caattatcga tacaatggca aacttttctg aatctaaatc catgatggca gttttcttca
      841 tgtttttcct gcttcttttg tcatcttcta gttcttcatc ttcaagttca ccaattttga
      901 aaaagatttt tattgaaagc ccttcctatg ctccgaatgc cttcaccttc gattcaactg
      961 ataaagggtt ctacacttcc gtccaagatg gccgagttat caaatatgaa gggccaaatt
     1021 caggcttcac tgacttcgcc tacgcatctc ccttctggaa caaagctttt tgtgagaaca
     1081 gcaccgatcc agagaaaaga ccattgtgtg ggaggacata tgatatttcc tatgactata
     1141 agaacagcca aatgtacatt gttgatggcc attaccatct ttgtgtggtt ggaaaagaag
     1201 gtgggtatgc cacacaacta gccacaagtg tgcaaggagt gccattcaaa tggctctatg
     1261 cagtaactgt tgatcagaga acagggattg tttatttcac tgatgttagc tccatacatg
     1321 atgacagtcc cgaaggtgtg gaagaaatca tgaatacaag tgatagaaca gggagattaa
     1381 tgaagtatga tccttcaaca aaagaaacca ccttattatt gaaagagcta catgttcccg
     1441 gcggtgcaga aatcagcgca gatggttcct ttgttgtagt agcagaattt ttaagcaatc
     1501 ggatagtgaa gtattggcta gaagggccaa agaaaggcag tgcagagttc ttagttacaa
     1561 tcccaaatcc aggaaatata aagaggaatt ctgatggcca tttttgggtg tcatctagtg
     1621 aagaattaga tggaggtcaa catggaagag ttgtttcaag aggaattaag tttgatggat
     1681 ttgggaatat tcttcaagtt ataccacttc caccaccata tgaaggtgaa cattttgaac
     1741 agattcaaga gcacgatggt ttgttataca ttggaagtct cttccatagc tctgtgggta
     1801 tattagtgta tgatgatcat gataacaagg gaaattctta tgtttctagc tgagcttctc
     1861 tagctagagt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga
     1921 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     1981 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     2041 atccagtact aaaatccaga tcgctactat tgtcttctgc acgaagtggt ttaaactatc
     2101 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     2161 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     2221 accacagggt tccccagatc aggcgctggc tgctgaaccc ccagccggaa ctgaccccac
     2281 aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc
     2341 gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt
     2401 ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg
     2461 cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca
     2521 tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac
     2581 ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac
     2641 cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga
     2701 caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca
     2761 aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa
     2821 cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat
     2881 cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt
     2941 cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt
     3001 gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt
     3061 gtgtttcagc aacgcggcct gcttggcctc gctgacctgt tttgccaggt cctcgccggc
     3121 ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa
     3181 acctgccgcc tcctgttcaa gacgacgcga acgctccacg gcggccgatg gcgcgggcag
     3241 ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat
     3301 cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt
     3361 ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc
     3421 aaacgtccga ttcattcacc ctccttgcgg gattgccccg actcacgccg gggcaatgtg
     3481 cccttattcc tgatttgacc cgcctggtgc cttggtgtcc agataatcca ccttatcggc
     3541 aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg tacttggtat tccgaatctt
     3601 gccctgcacg aataccagcg accccttgcc caaatacttg ccgtgggcct cggcctgaga
     3661 gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg
     3721 ccacatctag gatctgccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4021 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4081 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4141 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4201 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4261 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4321 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4501 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4561 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4621 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
     4681 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4741 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4801 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4861 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4921 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4981 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5041 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5101 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5161 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5221 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5281 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5341 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5401 tttcaatatt attgaagcat ttatcaaggt tattgtctca tgagcggata catatttgaa
     5461 tgtatttaga aaaataaaca aataggggtt ccgcgcacga attggccagc gctgccattt
     5521 ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg aggcccgcgt
     5581 tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg cggtcacgcg
     5641 cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa agcaggttaa
     5701 aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct ggattttctg
     5761 cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc actctgcccc
     5821 tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac
     5881 cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag
     5941 gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc
     6001 ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac gtccgcccct
     6061 catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg gccgcggtgt
     6121 ctcgcacacg gcttcgacgg cgtttctggc gcgttt
//
