LOCUS       sequence-349453-       11146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In planta gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   Harant A, Pai H, Sakai T, Kamoun S, Adachi H
  TITLE     A vector system for fast-forward studies of the HOPZ-ACTIVATED 
            RESISTANCE1 (ZAR1) resistosome in the model plant Nicotiana 
            benthamiana.
  JOURNAL   Plant Physiol. 2022 Jan 20;188(1):70-80. doi: 
            10.1093/plphys/kiab471.
  PUBMED    34633454
REFERENCE   2  (bases 1 to 11146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Physiol"; date: "2022-01-20- 20"; volume: "188"; issue: "1"; 
            pages: "70-80"
COMMENT     Sequence Label: pZA57
FEATURES             Location/Qualifiers
     source          1..11146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..56
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(179..767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(259..278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(938..1732)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(1733..1837)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2120..2314
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2380..3453)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3882..4511)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4775..4799
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4977..5156
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5009..5192
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5213..6007
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5267..5286)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5877..5896
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6033..6285
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7301..7646
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7589..7609
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             8635..8646
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             10278..10367
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             10308..10397
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     terminator      10408..11115
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 gggacacgaa gtggtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
       61 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
      121 ttcgtccatt tgtatgtgcc aaccacagga accgtaaaaa ggccgcgttg ctggcgtttt
      181 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      241 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      301 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      361 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      421 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      481 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      541 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      601 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
      661 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      721 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      781 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      841 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
      901 caatctaaag tatatatgag taaacttggt ctgacagcta aaacaattca tccagtaaaa
      961 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
     1021 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
     1081 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
     1141 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
     1201 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtcc tcttcccagt
     1261 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
     1321 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
     1381 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
     1441 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
     1501 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
     1561 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
     1621 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttgcgcagc
     1681 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atactcttcc
     1741 tttttcaata ttattgaagc atttatcaag gttattgtct catgagcgga tacatatttg
     1801 aatgtattta gaaaaataaa caaatagggg ttccgcgcac gaattggcca gcgctgccat
     1861 ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggagctcggt
     1921 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
     1981 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
     2041 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
     2101 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
     2161 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
     2221 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
     2281 cggctttggg ttcccaatgt acgtgctatc cacaggaaag aagctttttc gacctttttc
     2341 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
     2401 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
     2461 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
     2521 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
     2581 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
     2641 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
     2701 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
     2761 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
     2821 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
     2881 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
     2941 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
     3001 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
     3061 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
     3121 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
     3181 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
     3241 gggcggcttc ctaatcgacg acgcaccggc tgccggcggt tgccgggatt ctttgcggat
     3301 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
     3361 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
     3421 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
     3481 gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct
     3541 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
     3601 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
     3661 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
     3721 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
     3781 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
     3841 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
     3901 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
     3961 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
     4021 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
     4081 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
     4141 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
     4201 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
     4261 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
     4321 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
     4381 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
     4441 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
     4501 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
     4561 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
     4621 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
     4681 ccaggatttc atgcagccgc acggctgatg ggctgcctgt atcgagtggt gattttgtgc
     4741 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
     4801 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggat
     4861 gcagtgggcc ccactctgtg tgccgaattc ggatccggag agcggagaat taagggagtc
     4921 acgttatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac
     4981 cgcaacgttg aaggagccac tcagccgcgg gtttctggag tttaatgagc taagcacata
     5041 cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata
     5101 tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt
     5161 ctcatattca ctctcaatcc aaataatctg caccggatct ggatcgtttc gcatgattga
     5221 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5281 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5341 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tccaggacga
     5401 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5461 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5521 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5581 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5641 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5701 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgtatgcccg acggcgagga
     5761 tctcgtcgtg actcatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5821 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5881 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5941 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6001 cttctgagcg ggtctggatc tagagtcaag cagatcgttc aaacatttgg caataaagtt
     6061 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     6121 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     6181 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     6241 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgacgc tgcaagaatt
     6301 caagcttgga ggaattccaa tcccacaaaa atctgagctt aacagcacag ttgctcctct
     6361 cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc
     6421 acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg
     6481 agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac
     6541 aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc
     6601 caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct
     6661 aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat
     6721 tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga
     6781 cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc
     6841 tgacccacag atggttagag aggcctacgc agcaagtctc atcaagacga tctacccgag
     6901 taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
     6961 caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat
     7021 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt
     7081 ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg
     7141 aggatctaac agaactcgcc gtcaagactg gcgaacagtt catacagagt cttttacgac
     7201 tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca
     7261 aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt caacaaagga
     7321 taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga
     7381 cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca
     7441 ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     7501 tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt gacatctcca
     7561 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     7621 gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt tacaacaatt
     7681 accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc gatacaatgg
     7741 ttgatgctgt tgttacagtt tttcttgaaa agcttcttca tgttcttact gaagaatcta
     7801 gatttttgac taagtataga caacagttcg agaagcttaa gaacgaactt cttttcatgc
     7861 agtctttcct taaggacgct gaaagactta aaagaaagaa taacactttg aagggtgtga
     7921 tgtcttgcct tagggacttg atcttcgagg ctgaggagat cttggaggat tgccagaacc
     7981 agtctgctga ctctgataga gctactactt gcttccaccc taagaggttg tctcttaggc
     8041 accagactgg aaagtgtctt gcaaagatta atgataggat ttctgaaatt aagcagaata
     8101 tttctacata tcttggtgtg cctcttttgg aggagggttc tatggaggct cacaataacc
     8161 ttatgtctag atggacatct tccctttatg accacactca ggtagttggt ttggaaggtg
     8221 acactgagaa gataaaggat tggctatttg aagcaagaga tggtttactt accattgcat
     8281 ttgtgggtat gggaggactt ggtaaaacca ctcttgctca gaaagttttc aatgacaaaa
     8341 gggtggagga tcacttggag aggaggattt gggtgtctgt ttctcaaaca tttactgagg
     8401 aacaagtcat gagaagcata ttgaggagtt taggagatgc atgtgttggt gatgaccagt
     8461 gtgaactgtt aagaaaaata aaccagtacc ttttgggaaa gaggtttttg attgttatgg
     8521 atgatgtttg gagctgggac aatgcctggt ggcaaaaaat ctatactgga ctgcccaaag
     8581 gaaatgggag cactgttatt gtaacaacga gaaatgagct agtcgctcgc aagatgggag
     8641 ttacagaagc aagaatacat tggccaaaat tcctcaatga gcactacagt tggttactat
     8701 tccggaagat tgcatttgca ggaagtgcag gtgaatgcca tttccccgag ttggaggacg
     8761 tgggaaagga gattgtggaa aaatgcaagg gacttccttt agcaatcaaa gcagtaggag
     8821 gagtgatgct ttgcaagcca tcttactatc acgaatggag gcgtatttca aatcatttcc
     8881 gtgatgaatt gaaagaaaat gatgactcag tgatggcctc actacagttg agctatgatg
     8941 aactccctcc ttacttgaaa tcctgtttcc tctgtttttc actctttcct gaggattgtg
     9001 tcataccaaa agaccaactg atccgttggt ggattggaga aggtttcata cctctgagaa
     9061 gtggtaggtt atcgactgaa gtgggagaag attgtttctc acaactatcc aatcggtgtt
     9121 tgatagaagt tgttgataag gcttacaatg gtgtgatcca cacatgcaaa atgcatgaca
     9181 tggttcgtga cttggtgatc aaattagccg aggatgatgc atttttcacc ccagctgatg
     9241 caacttgtcg gcatttgggt attaagagtg agatgaattg gaagcaacta ttgagcaatc
     9301 aaaaattacg agcactgcta acaaccacca agagtggtga agttaacaaa atccattccg
     9361 acattgccaa gaagctgtgc aagagtcgac acctccaagt attggatctg tcaaaatcaa
     9421 ttttcgatgt gcctctttca agtttgttgg aaggaattgg atctgctaag cagctcactt
     9481 atctcagttt aagcaataca catccaatga ttggagttcc agcttccata tccaagcttg
     9541 aaaaattaca gattttggat ttcagctatt gccaaaatat gaaaatgctc ccctcttgcg
     9601 ttctgacatt tgaggaactt gctgttttgg atgtgaacaa ctgtggttcc cttgagtacc
     9661 ttccaaaagg attaagtagg ctttccaatc ttcaagtatt gcttgggttt aagcctgcaa
     9721 aattaagcca accaggaggt tgtcgtattg ctgagctcag aagcctgact cgactcagaa
     9781 ctctcagttt aagactaacc gaaaatgagg agattggaga cgatgagggg aacgcactgg
     9841 tagatctcca agaacttcaa tttttaacaa taagttgctt tggcagtcaa gataatggac
     9901 ttgcgacaaa acttggtaga ctttatcctc cacgacaact ccacgagctg attctcaaat
     9961 tttatccaag taagacaagt cctgaatggc ttaaccccaa tctctccccc atgttgcgat
    10021 atttatcaat catctctggt gatattacac aaatgcacga gaacttctgg ggtgatggta
    10081 gtactgcttg gaaaattgag ggcttaatgt tggaatcttt atcggatttg agattggaat
    10141 ggtcagcaat gcatcaagtc atgccttcgt taagaatact caaggttagc tggtgccctg
    10201 agttggagtc atttccaatt gaagatgcag ggtttagagg gggtctatgg aagaaggaag
    10261 aacataggaa cggttcggaa caaaagttga tctctgaaga ggaccttgag cagaaattga
    10321 tctctgagga agatcttgag cagaagctta tctcagagga ggatttggag caaaaactta
    10381 tttctgaaga ggacctttga gcttgtcctg ctttaatgag atatgcgaga agcctatgat
    10441 cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc
    10501 tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta
    10561 tttttatgaa taatattctc cgttcaattt actgattgta ccctactact tatatgtaca
    10621 atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag
    10681 cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca
    10741 gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc
    10801 aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc
    10861 cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc
    10921 taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg
    10981 ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt
    11041 caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat
    11101 gtcgaggctc agcaggaccg ctactagagg atgcacatgt gaccga
//
