LOCUS       sequence-349456-        4882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Coelho MA, Cooper S, Strauss ME, Karakoc E, Bhosle S, Goncalves E, 
            Picco G, Burgold T, Cattaneo CM, Veninga V, Consonni S, Dincer C, 
            Vieira SF, Gibson F, Barthorpe S, Hardy C, Rein J, Thomas M, Marioni
            J, Voest EE, Bassett A, Garnett MJ
  TITLE     Base editing screens map mutations affecting interferon-gamma 
            signaling in cancer.
  JOURNAL   Cancer Cell. 2023 Feb 13;41(2):288-303.e6. doi: 
            10.1016/j.ccell.2022.12.009. Epub 2023 Jan 19.
  PUBMED    36669486
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2022.12.009"; journalName: "Cancer Cell"; date: 
            "2023-02-13- 13"; volume: "41"; issue: "2"; pages: "288-303.e6"
COMMENT     Sequence Label: HA-SOCS1
FEATURES             Location/Qualifiers
     source          1..4882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..280
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     32..52
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     203..222
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        299..374
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        471..756
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          1007..1234
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             1255..1281
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1954..1971)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1960..2167
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2176..2316
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2176..2305
                     /label=AAV2 ITR
     rep_origin      2391..2846
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2478..2497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2688..2709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2863..2882)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2982..3004
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3042..3060)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3128..3232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3233..3301,3302..4093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3451..3470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4264..4852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4753..4772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctggcctttt gctggccttt tgctcacatg tgagggccta tttcccatga ttccttcata
       61 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
      121 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
      181 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
      241 tttcttggct ttatatatct tgtggaaagg acgaaacacc gggtcttcga gaagacctgt
      301 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      361 caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc
      421 cgtttttagc gcgtgcgcca attctgcaga caaatggctc tagaggtacc cgttacataa
      481 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      541 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      601 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      661 ggtaaatggc ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg
      721 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      781 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      841 ttattttgtg cagcgatggg ggcggggggg gggggggggg gggcggggcg gggcgaggcg
      901 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
      961 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1021 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1081 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     1141 gctgagcaag aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta
     1201 cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catgtacccg
     1261 tacgacgttc cggactacgc ttctggtggt tctgtagcac acaaccaggt ggcagccgac
     1321 aatgcagtct ccacagcagc agagcctaga aggcgcccgg aaccgtcatc ttctagtagt
     1381 tcatcaccag ctgcgcctgc cagaccccga ccttgtcctg ctgttcctgc cccggcgcct
     1441 ggggatacac acttccgcac attccgttcg cacgccgatt acaggcgcat cacgagggca
     1501 agcgcgctgc tcgatgcctg cggcttctac tggggacccc tgtctgtcca tggggcgcac
     1561 gagcggctgc gcgccgagcc cgtgggcacc ttcctggtgc gcgacagccg ccagcggaac
     1621 tgctttttcg cccttagcgt gaagatggcc tcgggaccca cgagcatccg cgtgcacttt
     1681 caggccggcc gctttcacct ggatggcagc cgcgagagct tcgactgcct cttcgagctg
     1741 ctggagcact acgtggctgc tccccgaaga atgcttggtg ctccgctgcg gcagcgaagg
     1801 gtccggcccc tgcaggaact gtgtcgacag cggattgtgg ccaccgtggg ccgcgagaac
     1861 ctggctcgca tccccctcaa ccccgtcctc cgcgactacc tgagctcctt ccccttccag
     1921 atttgagaat tctaactaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     1981 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2041 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     2101 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg
     2161 ctggggagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     2221 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     2281 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     2341 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     2401 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     2461 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     2521 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     2581 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     2641 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     2701 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     2761 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     2821 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     2881 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2941 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3001 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     3061 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3121 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3181 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3241 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3301 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3361 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3421 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3481 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3541 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3601 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3661 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3721 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3781 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3841 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3901 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggaa gccgcggtat
     3961 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4021 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4081 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4141 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4201 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4261 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4321 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4381 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4441 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4501 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4561 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4621 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4681 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4741 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4801 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4861 caacgcggcc tttttacggt tc
//
