LOCUS       sequence-34948-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Ra HJ, Harju-Baker S, Zhang F, Linhardt RJ, Wilson CL, Parks WC
  TITLE     Control of promatrilysin (MMP7) activation and substrate-specific 
            activity by sulfated glycosaminoglycans.
  JOURNAL   J Biol Chem. 2009 Oct 9. 284(41):27924-32.
  PUBMED    19654318
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Oct 9. 284(41):27924-32."
COMMENT     Sequence Label: pET23b-proMMP-7
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             811..828
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(881..899)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgctgccgc tgcctcagga ggcgggaggc
       61 atgagtgagc tacagtggga acaggctcag gactatctca agagatttta tctctatgac
      121 tcagaaacaa aaaatgccaa cagtttagaa gccaaactca aggagatgca aaaattcttt
      181 ggcctaccta taactggaat gttaaactcc cgcgtcatag aaataatgca gaagcccaga
      241 tgtggagtgc cagatgttgc agaatactca ctatttccaa atagcccaaa atggacttcc
      301 aaagtggtca cctacaggat cgtatcatat actcgagact taccgcatat tacagtggat
      361 cgattagtgt caaaggcttt aaacatgtgg ggcaaagaga tccccctgca tttcaggaaa
      421 gttgtatggg gaactgctga catcatgatt ggctttgcgc gaggagctca tggggactcc
      481 tacccatttg atgggccagg aaacacgctg gctcatgcct ttgcgcctgg gacaggtctc
      541 ggaggagatg ctcacttcga tgaggatgaa cgctggacgg atggtagcag tctagggatt
      601 aacttcctgt atgctgcaac tcatgaactt ggccattctt tgggtatggg acattcctct
      661 gatcctaatg cagtgatgta tccaacctat ggaaatggag atccccaaaa ttttaaactt
      721 tcccaggatg atattaaagg cattcagaaa ctatatggaa agagaagtaa ttcaagaaag
      781 aaatagtgaa agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      841 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      901 taaccctg
//
