LOCUS       sequence-349493-        6411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 1, position 5, transcriptional unit for transient 
            expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from 
            Catharanthus roseus driven by 35S promoter; contains a chloroplast 
            transit peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD1CB0116
FEATURES             Location/Qualifiers
     source          1..6411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    142..166
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        352..697
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     640..660
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    2136..2310
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2355..2379
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2535..3683)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(4019..4607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4099..4118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4778..5569,5570..5638))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5401..5420
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5639..5743)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(5830..6411,1..50)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc gcaaaggaga
       61 tcctgatctg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
      121 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
      181 acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggatgca gtgggcccca
      241 ctctgtgaag acaacagaga attcgcatgc ggaggtcaac atggtggagc acgacactct
      301 ggtctactcc aaaaatgtca aagatacagt ctcagaagat caaagggcta ttgagacttt
      361 tcaacaaagg ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
      421 catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
      481 aaaggctatc attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac
      541 gaggagcatc gtggaaaaag aagaggttcc aaccacgtct acaaagcaag tggattgatg
      601 tgacatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
      661 ctctatataa ggaagttcat ttcatttgga gaggacacgc tactgtataa gagctcattt
      721 ttacaacaat taccaacaac aacaaacaac aaacaacatt acaattacat ttacaattat
      781 cgataacatg gcttcttcta tgctttcttc tgctgctgtt gttgctactc gtgctagtgc
      841 tgctcaagct agtatggttg ctccttttac tggacttaag tctgctgctt cttttcctgt
      901 tactagaaag caaaacaacc ttgatattac ttctattgct agtaacggag gaagagttcg
      961 agcaatgacc aagaccaatt cccctgctcc ttctgtcatc acttgcaaag ctgctgtggt
     1021 atggaaatca ggggagccac caaaggtgga agagatacaa gttgatccac caaaggcctc
     1081 agaagttagg attaagatgc tttgtgccag tttgtgccac actgatttcc ttgcctgcaa
     1141 tggccttcct gttccattgt tccctcgcat tcctggacac gaaggagtcg gaatgatcga
     1201 gagcgttgga gaaaatgtta cgaacctaaa agaaggagac atagtgatgc cactttactt
     1261 gggagaatgt ggggaatgct tgaattgcaa atcaggaagg acaaatttgt gccacaaata
     1321 tcctttaggt tttagtggat tattgcttga tggaacatca agaatgtcaa ttggagagca
     1381 aaaagtatat caccatttca gctgttcgac atggtcagag tatatagtga ttgaagcagc
     1441 ttatgcagtg aaggtagatc caagggtttc tcttccacat gctagctttc tttgctgtgg
     1501 attcaccact ggttttggtg ctacttggag agatgtcaat gttgtcaagg gctctactgt
     1561 ggctgttcta ggccttggtg ctgttggact tggggctgtg caaggagcta aatcacaagg
     1621 agcatcaaga attataggtt tggatatcaa cgacaagaaa cgtgaaaagg gagaagcatt
     1681 tggaatgact gaatttataa atccaaaagg ttcaaacaaa tcaatttctg aactaataaa
     1741 tgaagcaact ggtggattag gacttgacta tgtctacgaa tgcactggag ttccagcttt
     1801 actcaatgaa gccattgaat cctctaaagt gggacttgga actgcagtat tgattggggc
     1861 aggacttgaa acaagtggag aaatcaaatt cattcctctt ctgtgtggta gaactgttaa
     1921 aggttcaatt tatggtggag taagacctaa atcagacctc cccactttaa ttgaaaagtg
     1981 cataaacaag gaaattccaa tggatgaact aatgactcat gaagtttcat tgtctgagat
     2041 aaacaaagga tttgagtacc ttaagcatcc tgattgtgtc aaagttgtta tcaagttcta
     2101 agcttctcta gctagagtcg atcgacaagc tcgagtttct ccataataat gtgtgagtag
     2161 ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga
     2221 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     2281 aaaccaaaat ccagtactaa aatccagatc gcttgtgttg tcttctgcac gaagtggttt
     2341 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     2401 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     2461 gcatgccaac cacagggttc cccagatcag gcgctggctg ctgaaccccc agccggaact
     2521 gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc
     2581 accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc
     2641 acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc
     2701 tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac
     2761 ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg
     2821 atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc
     2881 agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt
     2941 aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg
     3001 tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg
     3061 tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg
     3121 taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc
     3181 gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct
     3241 cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc
     3301 tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc
     3361 tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac
     3421 ttcgccaaac ctgccgcctc ctgttcaaga cgacgcgaac gctccacggc ggccgatggc
     3481 gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc
     3541 tggaccatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt
     3601 gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc
     3661 ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg
     3721 gcaatgtgcc cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc
     3781 ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc
     3841 cgaatcttgc cctgcacgaa taccagcgac cccttgccca aatacttgcc gtgggcctcg
     3901 gcctgagagc caaaacactt gatgcggaag aagtcggtgc gctcctgctt gtcgccggca
     3961 tcgttgcgcc acatctagga tctgccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4021 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4081 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4201 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4261 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4321 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4381 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4441 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4501 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4621 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4681 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4741 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4801 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4861 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4921 aaccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4981 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5041 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5101 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5161 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5221 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5281 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5341 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5401 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5461 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5521 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5581 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5641 tcttcctttt tcaatattat tgaagcattt atcaaggtta ttgtctcatg agcggataca
     5701 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacgaat tggccagcgc
     5761 tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag
     5821 gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg
     5881 gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag
     5941 caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg
     6001 attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac
     6061 tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt
     6121 gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt
     6181 aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc
     6241 gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt
     6301 ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc
     6361 cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg c
//
