LOCUS       sequence-349508-        7124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An improved vector for BacMam system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Guo W, Wang M, Chen L
  TITLE     A co-expression vector for baculovirus-mediated protein expression 
            in mammalian cells.
  JOURNAL   Biochem Biophys Res Commun. 2022 Feb 26;594:69-73. doi: 
            10.1016/j.bbrc.2022.01.056. Epub 2022 Jan 19.
  PUBMED    35074588
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "2022-02-26"; volume: "594:69-73. doi"; 
            pages: " 10.1016/j.bbrc.2022.01.056. Epub 2022 Jan 19"
COMMENT     Sequence Label: pBMCL1
FEATURES             Location/Qualifiers
     source          1..7124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          609..741
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     724..743
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             782..799
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    806..1394
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(859..879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1277..1288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    1521..1655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1558..1577)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1612..1631
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        join(2054..2059,2060..2076,2077..2082)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2252..2785
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  2852..3076
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(3210..3227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3381..3969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3461..3480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4140..4931,4932..5000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4763..4782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5001..5105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5131..5585)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5268..5289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5479..5498
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  5769..5934
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    6132..6180
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6205..6224
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6635..6667)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     promoter        complement(7001..7092)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(7059..7076)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcaaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      661 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      721 actttgcctt tctctccaca ggtgtaagga attcggatcc cgggcggccg ctagcctcga
      781 gcaccaccac caccaccact gagataatca acctctggat tacaaaattt gtgaaagatt
      841 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
      901 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
      961 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     1021 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     1081 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     1141 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     1201 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     1261 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     1321 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     1381 ggccgcctcc ccgcctggtc gagaagtact agaggatcat aatcagccat accacatttg
     1441 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     1501 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     1561 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1621 ccaaactcat caatgtatct tatcatgtct ggatctgatc agtaacaaca ccatttaaat
     1681 ggagtggtta caaatggagt ggttaattaa gttccggagt atacgtagcc aaccactaga
     1741 actatagcta gagtcctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     1801 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     1861 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     1921 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     1981 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     2041 ggcacgaacc cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca agtagcgtat
     2101 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     2161 ggttttcatg gcttgttatg actgtttttt tgtacagtct atgcctcggg catccaagca
     2221 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     2281 gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgcacatg
     2341 taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga
     2401 gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt
     2461 gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc
     2521 tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct atatctatga
     2581 tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca tcaatctcct
     2641 caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga
     2701 cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga
     2761 tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct tcccggccgc
     2821 ggagttgttc ggtaaattgt cacaacgccg cgaatatagt ctttaccatg cccttggcca
     2881 cgcccctctt taatacgacg ggcaatttgc acttcagaaa atgaagagtt tgctttagcc
     2941 ataacaaaag tccagtatgc tttttcacag cataactgga ctgatttcag tttacaacta
     3001 ttctgtctag tttaagactt tattgtcata gtttagatct attttgttca gtttaagact
     3061 ttattgtccg cccacacccg cttacgcagg gcatccattt attactcaac cgtaaccgat
     3121 tttgccaggt tacgcggctg gtctatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     3181 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4081 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4141 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4201 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4261 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4321 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4381 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4441 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4741 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5101 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     5161 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     5221 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     5281 agagtccact attaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     5341 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     5401 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     5461 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     5521 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     5581 cgcgtcccat tcgccattca ggctgcaaat aagcgttgat attcagtcaa ttacaaacat
     5641 taataacgaa gagatgacag aaaaattttc attctgtgac agagaaaaag tagccgaaga
     5701 tgacggtttg tcacatggag ttggcaggat gtttgattaa aaacataaca ggaagaaaaa
     5761 tgccccgctg tgggcggaca aaatagttgg gaactgggag gggtggaaat ggagttttta
     5821 aggattattt agggaagagt gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa
     5881 tacgaaaatt aaaaatgaca aaatagtttg gaactagatt tcacttatct ggttcggatc
     5941 tcctaggctc aagcagtgat cagtggctat ggcagggctt gccgccccga cgttggctgc
     6001 gagccctggg ccttcacccg aacttggggg ttggggtggg gaaaaggaag aaacgcgggc
     6061 gtattggtcc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
     6121 cccgcgttta tgaacaaacg acccaacacc cgtgcgtttt attctgtctt tttattgccg
     6181 tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctcccccatc
     6241 tcccgctcta gtacttctcg acaagctttt aggccagggc tttaccctcg gtcttggagg
     6301 tgacgtccac ctggacaccc acgaacttgg acacgcgacc gtcgggagtc ttgatggggg
     6361 tcacagtcag caggttccag aaaggggtac cgtccttgcg gtagttcagc agacgcacgg
     6421 agcaggcttc acccttcttg atagcgtcgc ggatcttctg cacctccttg gggtcggtac
     6481 cttcaccctg caggaagcgg gcgttgtggc ccagcacttc gtcggggccg taaccagtca
     6541 tggcgtagaa gccctcggaa gcgtacacca gggggcagtc gggcagagta gcgtcagcca
     6601 ccacgaaggt gtgacgcaga ccggcaccgc cacctttacc caggcggttc atctcgatgt
     6661 cagtatatat ctgacgcttc ttggtgtgct tcagcttgat gcacaggtgg atacccacgc
     6721 gcaggaccag gaacagaccg atgatcaggc cgatgatgaa gaagaaagaa gcgatgctgg
     6781 acttccagct gctgaaccag ccttcgacca gttcgatagg gttcttgctc agaccggtgt
     6841 caccagcgaa ggcggagtga gcagcagcag ccagcagcac gtacagcacg atagcgctca
     6901 ccatcttgct agtgtgctcc ttgttgaaac cctggtggct ctggttcacc agcagcatag
     6961 atccgcgccc gatggtggga cggtatgaat aatccggaat atttataggt ttttttatta
     7021 caaaactgtt acgaaaacag taaaatactt atttatttgc gagatggtta tcattttaat
     7081 tatctccatg atctattaat acttaattaa caacaccatt tgtt
//
