LOCUS       sequence-349524-        6600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 1, position 1, transcriptional unit for transient 
            expression of tryptophan decarboxylase (CrTDC) from Catharanthus 
            roseus driven by 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD1CB0773
FEATURES             Location/Qualifiers
     source          1..6600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    132..156
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        342..687
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     630..650
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    2315..2489
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2534..2558
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2714..3862)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(4198..4786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4278..4297)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4957..5748,5749..5817))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5580..5599
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5818..5922)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(6009..6600,1..40)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 cgccagccca gcggcgaggg caaccagccc ggtgagcgtc gcaaaggaga tcctgatctg
       61 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
      121 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
      181 aacacattgc ggacgttttt aatgtactgg ggtggatgca gtgggcccca ctctgtgaag
      241 acaatgccga attcggatcc ggaggtcaac atggtggagc acgacactct ggtctactcc
      301 aaaaatgtca aagatacagt ctcagaagat caaagggcta ttgagacttt tcaacaaagg
      361 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
      421 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
      481 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
      541 gtggaaaaag aagaggttcc aaccacgtct acaaagcaag tggattgatg tgacatctcc
      601 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
      661 ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctcattt ttacaacaat
      721 taccaacaac aacaaacaac aaacaacatt acaattacat ttacaattat cgatacaatg
      781 ggcagcattg attcaacaaa tgtagccatg tccaattctc cagttggaga atttaagcca
      841 cttgaagctg aggaattccg aaaacaagcc catcgtatgg tagatttcat agccgattat
      901 tacaaaaatg tggaaacata tccggtcctt agcgaagtcg aacctggata tctccgaaaa
      961 cgtatccccg aaaccgctcc ttacctcccc gaaccacttg acgacatcat gaaagatatt
     1021 cagaaggata ttatcccagg aatgacaaat tggatgagcc ctaattttta tgcatttttt
     1081 cctgccactg ttagttcagc tgccttttta ggagaaatgt tgtctactgc cctaaattca
     1141 gtaggcttta cttgggtttc ttcaccagcc gccaccgaat tagaaatgat tgttatggat
     1201 tggttggctc agatccttaa actccccaaa tctttcatgt tttcaggtac cggtggcggc
     1261 gtcatccaaa acaccactag cgagtccatt ctttgtacaa tcattgccgc ccgggaaagg
     1321 gccctggaga agctcggtcc cgatagtatt ggaaaacttg tctgttacgg atccgatcaa
     1381 acccatacca tgttccccaa aacttgcaaa ttggcgggaa tttatccgaa taatattagg
     1441 ttaataccta cgaccgtcga aacggatttc ggcatctcac ctcaagttct acgaaaaatg
     1501 gtcgaggatg acgtggcggc cggatatgta ccgctgttct tatgcgctac cctgggtacc
     1561 acctcgacca cggctaccga tcctgtggac tcactttctg aaatcgctaa cgagtttggt
     1621 atttggatcc acgtggatgc tgcttatgcg ggaagcgcct gtatatgtcc cgagtttaga
     1681 cattacttgg atggaatcga acgagttgac tcactgagtc tgagtccaca caaatggcta
     1741 ctcgcttact tagattgcac ttgcttgtgg gtcaagcaac cacatttgtt actaagggca
     1801 ctcactacga atcctgagta tttaaaaaat aaacagagtg atttagacaa agttgtggac
     1861 ttcaaaaatt ggcaaatcgc aacgggacga aaatttcggt cgctgaaact ttggctcatt
     1921 ttacgtagct atggagttgt taatttacag agtcatattc gttctgacgt cgcaatgggc
     1981 aaaatgttcg aagaatgggt tagatcagac tccagattcg aaattgtggt accgagaaac
     2041 ttttctcttg tttgttttag attaaaacct gacgtttcga gtttacatgt agaagaagtg
     2101 aataagaaac ttttggacat gcttaactcg acgggacgag tttatatgac tcatactatt
     2161 gtgggaggca tatacatgct aagactggct gttggctcgt cactaactga agaacatcat
     2221 gtacgccgtg tttgggattt gattcaaaaa ttaaccgatg atttgctcaa agaagcttga
     2281 gcttctctag ctagagtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt
     2341 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
     2401 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     2461 aaccaaaatc cagtactaaa atccagatcg ctgcaattgt cttctgcacg aagtggttta
     2521 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     2581 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     2641 catgccaacc acagggttcc ccagatcagg cgctggctgc tgaaccccca gccggaactg
     2701 accccacaag gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca
     2761 ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca
     2821 cgcgggtgga atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct
     2881 cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact
     2941 tctcccatat gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga
     3001 tcaggacctg gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca
     3061 gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta
     3121 ggcgcgacag gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt
     3181 cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt
     3241 actccaacac ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt
     3301 aggtgatctt cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg
     3361 ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc
     3421 gcatcgtgtc cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct
     3481 gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct
     3541 cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact
     3601 tcgccaaacc tgccgcctcc tgttcaagac gacgcgaacg ctccacggcg gccgatggcg
     3661 cgggcagggc agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct
     3721 ggaccatcga gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg
     3781 cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct
     3841 tccggtcaaa cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg
     3901 caatgtgccc ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct
     3961 tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc
     4021 gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa atacttgccg tgggcctcgg
     4081 cctgagagcc aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat
     4141 cgttgcgcca catctaggat ctgccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4201 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4261 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4321 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4381 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4441 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4501 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4561 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4621 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4681 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4741 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4801 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4861 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4921 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4981 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5041 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5101 accacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5161 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5221 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5281 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5341 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5401 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5461 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5521 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5581 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5641 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5701 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5761 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5821 cttccttttt caatattatt gaagcattta tcaaggttat tgtctcatga gcggatacat
     5881 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacgaatt ggccagcgct
     5941 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg
     6001 cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg
     6061 tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc
     6121 aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga
     6181 ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact
     6241 ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg
     6301 tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta
     6361 aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg
     6421 agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc
     6481 cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc
     6541 gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc
//
