LOCUS       sequence-349578-       13634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 2 plasmid containing multiple transcriptional units for 
            transient expression of P19 (cytosol, driven by nosP), along with 
            CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13634)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 13634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD2KN0908
FEATURES             Location/Qualifiers
     source          1..13634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    132..156
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        321..500
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    536..591
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      1124..1376
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    1411..1444
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    1470..1503
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    1533..1566
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    1592..1625
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        1642..1688
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      4068..4320
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     protein_bind    4353..4386
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    4412..4445
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    4475..4508
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    4534..4567
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        4584..4630
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      5930..6637
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    6673..6706
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6732..6765
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6795..6828
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6854..6887
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        6904..6950
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             8601..8612
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      8922..9122
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    9186..9210
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9519..10148
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10577..11650
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11716..11910
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        11972..12076
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12077..12871
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13042..13630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13531..13550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccagcaacgc ggccttttta cggttcctgc tcggatctgt tggaccggac agtagtcatg
       61 gttgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
      121 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
      181 aacacattgc ggacgttttt aatgtactgg ggttgaacac tctgtgccga attcggatcc
      241 ggagagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg ggacaagccg
      301 ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc gcgggtttct
      361 ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca aaagtcgcct
      421 aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac gttccataaa
      481 ttcccctcgg tatccaatta gagtctcata ttcactctca tactgtattt ttacaacaat
      541 taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta caatggaacg
      601 agctatacaa ggaaacgatg ctagggaaca agcttatggt gaacgttgga atggaggatc
      661 aggaagttcc acttctccct tcaaacttcc tgacgaaagt ccgagttgga ctgagtggcg
      721 gctacataac gatgagacta tttcgaatca agataatccc cttggtttca aggaaagctg
      781 gggtttcggg aaagttgtat ttaagagata tctcagatac gacgggacgg aaacttcact
      841 gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat ctcgatttct
      901 cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta gtgtcaccat
      961 ttctggaggg tcgcgaactc ttcagcatct cagtgaaatg gcaattcggt ctaagcaaga
     1021 actgctacag cttaccccag tcaaagtgga aagtgatgta tcaagaggat gccctgaagg
     1081 tgttgaaacc ttcgaagaag aaagcgagta agcttgtcaa gcagatcgtt caaacatttg
     1141 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     1201 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     1261 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     1321 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacg
     1381 ctgcaagaat tcaagcttgg agcggtgcgg gtgccagggc gtgcccttgg gctccccggg
     1441 cgcgtactcc actccccgag ccggtgcggg tgccagggcg tgcccttggg ctccccgggc
     1501 gcgtactcca ctccccccat ggtgcggtgc gggtgccagg gcgtgccctt gggctccccg
     1561 ggcgcgtact ccactccccg agccggtgcg ggtgccaggg cgtgcccttg ggctccccgg
     1621 gcgcgtactc cactcccctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     1681 ggagaggact ccggtatttt tacaacaatt accacaacaa aacaaacaac aaacaacatt
     1741 acaatttact attctagtcg aatactgtat aagagctcat ttttacaaca attaccaaca
     1801 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgataaca tggcttcttc
     1861 tatgctttct tctgctgctg ttgttgctac tcgtgctagt gctgctcaag ctagtatggt
     1921 tgctcctttt actggactta agtctgctgc ttcttttcct gttactagaa agcaaaacaa
     1981 ccttgatatt acttctattg ctagtaacgg aggaagagtt cgagcaatgg ctgctgctca
     2041 tgggcatgct cataatgttg atgaaaatta ccaagtccta gtgaagaagc agcctttgaa
     2101 tttttcagga gataagcctt ttactcctat tttggatacc atcaactacc ctacccatat
     2161 gaagaatctc tctcttcagg agcttcaaca attagctgac gaattaaggg aggagatagt
     2221 gtattctgtg gccaaaacgg gtggtcactt aagttccagc ctaggtgtgg ccgagctcac
     2281 tgtggcactc caccacgtgt tcgaaacccc aaatgataaa atcatatggg atgttggcca
     2341 ccagacatat gggcataaga ttctgacagg gagaaggtca aagatgcata ccatgagaca
     2401 aacttgcgga cttgcaggtt tccccaaaag agatgaaagt gcccatgatg catttggagt
     2461 tggccatagt tccaccagca tttctgccgg tttaggaatg gcaataggac gagatttatt
     2521 aggtaagaaa aatcatgtaa taacggtaat aggagatgga gctatgacag ccggacaagc
     2581 atatgaagcc atgaataatg ctggatatct tgattctaat ctcataatca tattgaatga
     2641 taacaaacaa gtttctctcc cgactgccac aattgatggt cctgcacctc ctactggtgc
     2701 tctaagcaat gccttaacta ggcttcaatc tagtagaaaa tttcgactac ttcgggaggc
     2761 tgcaaaaggt gtgacaaagc aattaggggg acaagttcat gaaatggcag ctaaagtgga
     2821 tacatatatg agagggatga atagccaaag ggcttcctta tttgaagatt taggaatgta
     2881 ttatattggt ccacttgatg gtcacaatat ggaagatctt gttgatattt tagagaaagt
     2941 aaaatctgtt cctgcaacgg gccctgttct tattcatatt attactgaga aaggaaaagg
     3001 atatcctcct gctgaagctg ccgccgacaa aatgcatggg gtggtgaaat ttgagccaat
     3061 gacaggaaaa caattcaagt caaagtcaaa aacaaagtca tatacaaatt attttgctga
     3121 gagtttaata agtgaagcta gggaagatga taagattatt gccattcatg ctgctatggg
     3181 aggaggaaca ggactcaatt tattccagaa acactttcct gaacgatgct ttgatgttgg
     3241 gatagctgag caacatgctg tcacttttgc tgctggctta gcttctgaag gactcaagcc
     3301 tttttgtgct atttactcat ctttcttaca aagaggttat gatcaggtgg ttcatgatgt
     3361 ggaccttcaa aaaattccag taaggtttgc tatggataga gcagggctgg tgggtgcaga
     3421 tggacccact cattgcggtg catttgacac aacttttatg gcttgtttgc ctaatatggt
     3481 ggtcatggct ccttcagatg aaaccgaatt gatgcacatg gttgccacag cagctgccat
     3541 tgatgatagg cctagttgct ttcgatatcc cagaggaaat ggcattggtg caattcttcc
     3601 accaaacaac aaaggaactc cattacagat tggtaaagga agaatattgt tggagggaac
     3661 aagagttgcc attcttgggt atggagcaat ggtgcaaaac tgtttaatgg ctgctcagct
     3721 tcttgaaatt aatggaatat cagctacagt tgttgatgcc cgattttgca aacctcttga
     3781 tggagatttg attagaaaat tggctcaaca acatgaagtc cttatcactg ttgaagaagg
     3841 atccattggt ggttttggtt cccatgtggc acagttcttg gctttgaatg gattgcttga
     3901 tggaaattta aagtggaggg caatgatgct tcctgacaga tatattgatc atggagctca
     3961 aaatgaccaa attgaagagg ctggcctaac tccaaagcat attgctggca ctgtgttttc
     4021 tctactcagc aaaaacaaga atactcttca agttctccag ttttaggctt ggactcccat
     4081 gttggcaaag gcaaccaaac aaacaatgaa tgatccgctc ctgcatatgg ggcggtttga
     4141 gtatttcaac tgccatttgg gctgaattgt agacatgctc ctgtcagaaa ttccgtgatc
     4201 ttactcaata ttcagtaatc tcggccaata tcctaaatgt gcgtggcttt atctgtcttt
     4261 gtattgtttc atcaattcat gtaacgtttg cttttcttat gaattttcaa ataaattatc
     4321 cgctactaga attcgagctc ggagcggtgc gggtgccagg gcgtgccctt gggctccccg
     4381 ggcgcgtact ccactccccg agccggtgcg ggtgccaggg cgtgcccttg ggctccccgg
     4441 gcgcgtactc cactcccccc atggtgcggt gcgggtgcca gggcgtgccc ttgggctccc
     4501 cgggcgcgta ctccactccc cgagccggtg cgggtgccag ggcgtgccct tgggctcccc
     4561 gggcgcgtac tccactcccc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
     4621 ttggagagga ctccggtatt tttacaacaa ttaccacaac aaaacaaaca acaaacaaca
     4681 ttacaattta ctattctagt cgaatactgt ataagagctc atttttacaa caattaccaa
     4741 caacaacaaa caacaaacaa cattacaatt acatttacaa ttatcgataa catggcttct
     4801 tctatgcttt cttctgctgc tgttgttgct actcgtgcta gtgctgctca agctagtatg
     4861 gttgctcctt ttactggact taagtctgct gcttcttttc ctgttactag aaagcaaaac
     4921 aaccttgata ttacttctat tgctagtaac ggaggaagag ttcgagcaat gtgctcaaac
     4981 acaaatgccc agttggccaa tttgtctgaa caagtaaagg ggaaggtcac tgaatttgat
     5041 ttcaaggagt acatgcgttc caaggcaatg tcagtgaatg aggcactgga cagggctgtc
     5101 ccacttcgtt atcctgaaaa aatacatgaa gctatgaggt attctcttct agcaggaggg
     5161 aagcgagttc gtcctattct gtgcattgca gcatgtgagc ttgtgggagg gagcgaggag
     5221 ctcgccatgc caactgcctg tgcaatggaa attatccaca caatgtcttt gattcatgat
     5281 gacttgcctc ccatggacaa tgatgattta cgccgaggta agcctacaaa ccacaaggtg
     5341 ttcggtgaag gcactgctgt tcttgcgggg gatgcacttc tttcatttgc atttgagcac
     5401 attgcagtgt ccacaagcaa gactgtggag tctgataggg ttttgagggt ggtatctgaa
     5461 ttgggtagag caataggctc ggaaggggtt gcaggtgggc aggttgctga tattaccagt
     5521 caggggaatc cttctgttgg ccttgagact ctggaatgga ttcacattca caagactgca
     5581 gtgctcttgg agtgctccgt tgctagtggg gcgatcatcg gtggtgcttc tgatgatgag
     5641 attgagagag ttcgaaagta tgcccgttgc gtggggcttc tgtttcaggt tgtggatgac
     5701 attctcgatg tcacgaaatc atcagaagaa ttgggtaaga ctgcagcaaa ggatttgctt
     5761 agtgataagg ccacttatcc caagctcatg ggtttggaga aagcaaagga gtttgctgat
     5821 gaattgttgg gcaaagctaa ggaggagtta tctttcttca acccaactaa ggctgcacct
     5881 ctgttgggtc ttgcagatta cattgcacaa agacagaact gagcttgtcc tgctttaatg
     5941 agatatgcga gaagcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt
     6001 aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa
     6061 tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg
     6121 taccctacta cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt
     6181 tatagcgaca tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa
     6241 tccaatttta aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt
     6301 attcaaattt caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa
     6361 taacgctcac tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa
     6421 gttgagtatt ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca
     6481 cggcggccgg gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt
     6541 gggccccaaa tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc
     6601 agggcgaatt ttgcgacaac atgtcgaggc tcagcaggac cgctttacga attcccatgg
     6661 ggagcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccactccccg
     6721 agccggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cactcccccc
     6781 atggtgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccactccc
     6841 cgagccggtg cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccactcccc
     6901 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt
     6961 tttacaacaa ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt
     7021 cgaatactgt ataagagctc atttttacaa caattaccaa caacaacaaa caacaaacaa
     7081 cattacaatt acatttacaa ttatcgataa catggcttct tctatgcttt cttctgctgc
     7141 tgttgttgct actcgtgcta gtgctgctca agctagtatg gttgctcctt ttactggact
     7201 taagtctgct gcttcttttc ctgttactag aaagcaaaac aaccttgata ttacttctat
     7261 tgctagtaac ggaggaagag ttcgagcaat gtctctgcct ttggcaactc cattgatcaa
     7321 agacaatgaa tctctcatca agttcttgcg ccaacccctg gtgcttcctc atgaggttga
     7381 tgacagcaca aaaaggaggg aattgttgga aagaacaaga aaagaactag aattaaatgc
     7441 agaaaaacca ttggaggcct tgaagatgat agatataatt caaagattgg gattatcata
     7501 tcattttgaa gatgatatta attcaattct cacaggattt tcaaatatta gcagccaaac
     7561 tcatgaagat ctcctcactg cttcactttg ttttcgattg cttcgacaca atgggcataa
     7621 gatcaatcct gatatattcc aaaaattcat ggacaacaat ggaaagttta aagattcatt
     7681 aaaggatgac acattaggca tgttaagctt atatgaagct tcatatttgg gagccaatgg
     7741 agaagaaata ttgatggaag cccaagaatt caccaaaact cacctgaaaa actcattgcc
     7801 agccatggca ccatctcttt ctaagaaggt ttctcaagct ttagagcaac caagacatag
     7861 aagaatgttg aggttagaag ctagaagatt tattgaagaa tatggtgctg aaaatgacca
     7921 taatccagac cttcttgagc ttgcaaaatt ggattataac aaagtccaat ctctacacca
     7981 aatggaattg tctgagataa caaggtggtg gaaacaatta gggcttgtgg ataaactcac
     8041 ctttgctcga gatcgacccc ttgaatgctt tctttggaca gtgggattat taccagagcc
     8101 taagtattca ggttgcagaa ttgagcttgc aaaaaccata gccattttgc ttgtcattga
     8161 tgatatcttt gatactcatg gtaccctaga tgagcttctt ctattcacta atgccattaa
     8221 aagatgggat cttgaggcca tggaagattt accagaatat atgagaattt gttacatggc
     8281 attgtacaat actactaatg aaatttgcta taaagttctt aaggaaaatg gttggagtgt
     8341 tcttccttac ctaaaggcaa cgtggattga tatgattgaa ggattcatgg ttgaagcaga
     8401 atggttcaat tctgattatg taccaaacat ggaagaatat gtagaaaatg gagttagaac
     8461 agcaggatca tatatggcct tagtccattt gttctttcta atagggcaag gtgtcactga
     8521 agataatgtg aaattactga ttaaacccta tccaaagctc ttttcctcct caggaagaat
     8581 ccttcgcctt tgggatgatt tgggaactgc aaaggaggaa caagaaagag gagacttggc
     8641 atcaagcata caattgttta tgagagagaa agagataaaa tcagaagaag aaggaaggaa
     8701 aggaatattg gaaattatag agaatttatg gaaagaattg aatggagaat tagtttatag
     8761 agaagaaatg cctcttgcaa taatcaagac agcattcaac atggcaagag cttcccaagt
     8821 tgtgtatcaa catgaagagg acacctattt ttcaagtgta gataattatg taaaagcttt
     8881 gttttttaca ccttgttttt aagcttgatc ccccgtcgac agctagctat atcatcaatt
     8941 tatgtattac acataatatc gcactcagtc tttcatctac ggcaatgtac cagctgatat
     9001 aatcagttat tgaaatattt ctgaatttaa acttgcatca ataaatttat gtttttgctt
     9061 ggactataat acctgacttg ttattttatc aataaatatt taaactatat ttctttcaag
     9121 atcgctcaga gaggatgcac atgtgaccga gggacacgaa gtgatccgtt taaactatca
     9181 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
     9241 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     9301 ccacagggtt cccctcggga gtcagccgtg cggctgcatg aaatcctggc cggtttgtct
     9361 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     9421 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     9481 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     9541 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     9601 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     9661 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     9721 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     9781 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     9841 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     9901 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     9961 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
    10021 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
    10081 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
    10141 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
    10201 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
    10261 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
    10321 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
    10381 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
    10441 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
    10501 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
    10561 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
    10621 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
    10681 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
    10741 atcccggcaa ccgccggcag ccggtgcgtc gtcgattagg aagccgccca agggcgacga
    10801 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
    10861 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
    10921 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
    10981 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
    11041 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
    11101 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
    11161 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
    11221 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
    11281 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agattacaga
    11341 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
    11401 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
    11461 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
    11521 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
    11581 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
    11641 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
    11701 aaaaagcttc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
    11761 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
    11821 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
    11881 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaacagct
    11941 gcaaaaagcg cccacatcaa ggctccgagt gcgcggaacc cctatttgtt tatttttcta
    12001 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    12061 ttgaaaaagg aagagtatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga
    12121 aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt
    12181 gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta
    12241 tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa
    12301 ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg
    12361 cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta
    12421 tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag
    12481 cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga
    12541 ttgcgaaaac tgggaagagg acactccatt taaagatccg cgcgagctgt atgatttttt
    12601 aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa
    12661 catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc
    12721 ggacaagtgg tatgacattg ccttctgcgt ccggtcgctc agggaggata tcggggaaga
    12781 acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa
    12841 atattatatt ttactggatg aattgtttta gctgtcagac caagtttact catatatact
    12901 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    12961 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    13021 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    13081 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    13141 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    13201 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    13261 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    13321 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    13381 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    13441 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    13501 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    13561 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    13621 cctatggaaa aacg
//
