LOCUS       sequence-349579-       16627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level P, position 1, plasmid containing multiple 
            transcriptional units for transient expression of CrISY, NmMLPL, 
            CrG8H, CrGOR, CrIO, and Cr7-DLGT driven by 4x opaatB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16627)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 16627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQDPKN0892
FEATURES             Location/Qualifiers
     source          1..16627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    144..168
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    275..308
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    334..367
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    397..430
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    456..489
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        506..552
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    642..697
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     polyA_signal    1900..2074
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    2106..2139
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2165..2198
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2228..2261
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2287..2320
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        2337..2383
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    2473..2528
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      3014..3266
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     protein_bind    3299..3332
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    3358..3391
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    3421..3454
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    3480..3513
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        3530..3576
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    3666..3721
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      5212..5919
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    5955..5988
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6014..6047
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6077..6110
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6136..6169
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        6186..6232
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    6322..6377
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      7535..7735
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    7768..7801
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    7827..7860
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    7890..7923
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    7949..7982
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        7999..8045
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    8135..8190
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     polyA_signal    9774..9948
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    9980..10013
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    10039..10072
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    10102..10135
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    10161..10194
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        10211..10257
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    10347..10402
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      11865..12117
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    12191..12215
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12524..13153
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13582..14655
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14721..14915
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        14977..15081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             15082..15876
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      join(16047..16627,1..8)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16536..16555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatggaaaaa cgccagcaac gcggcctttt tacggttcct gctcggatct gttggaccgg
       61 acagtagtca tggttgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
      121 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
      181 agacaactta ataacacatt gcggacgttt ttaatgtact ggggttgaac actctgtgga
      241 agacaatgcc gaattcggat ccggagcggt gcgggtgcca gggcgtgccc ttgggctccc
      301 cgggcgcgta ctccactccc cgagccggtg cgggtgccag ggcgtgccct tgggctcccc
      361 gggcgcgtac tccactcccc ccatggtgcg gtgcgggtgc cagggcgtgc ccttgggctc
      421 cccgggcgcg tactccactc cccgagccgg tgcgggtgcc agggcgtgcc cttgggctcc
      481 ccgggcgcgt actccactcc ccttcgcaag acccttcctc tatataagga agttcatttc
      541 atttggagag gactccggta tttttacaac aattaccaca acaaaacaaa caacaaacaa
      601 cattacaatt tactattcta gtcgaatact gtatttttac aacaattacc aacaacaaca
      661 aacaacaaac aacattacaa ttactattta caattacaat gagttggtgg tggaagaggt
      721 ccattggtgc tggaaagaac ttaccaaacc agaacaaaga aaatggagtt tgtaaaagct
      781 acaagagtgt ggcactagta gtaggagtca ccggcattgt tggcagtagt ttagccgaag
      841 ttctaaagct accagacacc cctggaggtc cctggaaggt atacggcgtg gcaaggcgcc
      901 catgccctgt atggctagcc aaaaaaccag ttgagtacat ccaatgtgat gtctcagata
      961 atcaagaaac aatttctaaa ttatctccac ttaaggacat aactcatata ttctatgttt
     1021 cttggattgg atcagaagat tgccaaacaa atgctacaat gttcaaaaac atccttaatt
     1081 cagtgattcc taatgcttca aatctccaac atgtgtgtct ccaaacagga atcaaacatt
     1141 attttggtat ttttgaagaa ggttctaaag ttgtaccaca tgattcgcct tttaccgagg
     1201 atttacctag gctaaatgtc ccaaatttct accatgatct tgaagatata ctgtatgaag
     1261 aaactggcaa gaacaattta acatggtctg tccatagacc agctctggtt ttcgggtttt
     1321 ccccatgtag tatgatgaac attgtcagta cattatgcgt ttacgccaca atttgtaaac
     1381 atgaaaataa ggccttggtt tatccaggta gtaaaaattc atggaattgt tatgctgatg
     1441 ctgttgatgc agatttagta gccgagcacg aaatttgggc agcagttgat cctaaggcta
     1501 aaaatcaagt attgaattgt aacaatgggg atgtttttaa atggaaacat atttggaaga
     1561 aattagcaga ggaatttggg attgaaatgg tgggatatgt tgaaggtaaa gaacaagtta
     1621 gtttggctga gttaatgaag gataaagatc aagtttggga tgaaattgtt aagaagaaca
     1681 atttagtacc tactaaattg aaagaaattg ctgccttttg gtttgctgat attgcttttt
     1741 gttctgagaa tttgattagt agtatgaaca aaagtaagga attagggttc ttgggtttta
     1801 ggaattcgat gaaatcgttt gtttcttgta ttgataagat gagggattat aggtttattc
     1861 cttaggcttc tctagctaga gtcgatcgac aagctcgagt ttctccataa taatgtgtga
     1921 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
     1981 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     2041 cctaaaacca aaatccagta ctaaaatcca gatcgctgca agaattcaag cttggagcgg
     2101 tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccactcc ccgagccggt
     2161 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cccatggtgc
     2221 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccact ccccgagccg
     2281 gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccactc cccttcgcaa
     2341 gacccttcct ctatataagg aagttcattt catttggaga ggactccggt atttttacaa
     2401 caattaccac aacaaaacaa acaacaaaca acattacaat ttactattct agtcgaatac
     2461 tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca attactattt
     2521 acaattacaa tggcttcgaa gcttgaaata gaaattgagt tgaaaagtga tgtagaaaag
     2581 atgtggaaac acttcaaaga attcaccaaa ttattcccca aggcttgtcc tcatctttac
     2641 gagaagattg atgtgatcga gggagatggg atatcagttg gaacaatctt tgtcagtacc
     2701 ctgaaaccaa cagagttgaa ccccgtggtt atggtgacga aggagaagat tgatttttta
     2761 gatgatgaga acaaaatgtt gaggtatagt tacatggaag gggagatatt aaagaattac
     2821 aagaatttga ggggcacagt gcatatgagc agcagcaaaa gtggtggaac tatattcaaa
     2881 tattcaggag aattcgaaaa ggcaaatgaa caagtccctg atcctgtttt cttcaaagat
     2941 tttatggtca tagttttcca aggtttggat gattatattc tcaagggcat gaaccataca
     3001 tgtcaaaatt aagcttggac tcccatgttg gcaaaggcaa ccaaacaaac aatgaatgat
     3061 ccgctcctgc atatggggcg gtttgagtat ttcaactgcc atttgggctg aattgtagac
     3121 atgctcctgt cagaaattcc gtgatcttac tcaatattca gtaatctcgg ccaatatcct
     3181 aaatgtgcgt ggctttatct gtctttgtat tgtttcatca attcatgtaa cgtttgcttt
     3241 tcttatgaat tttcaaataa attatccgct actagaattc gagctcggag cggtgcgggt
     3301 gccagggcgt gcccttgggc tccccgggcg cgtactccac tccccgagcc ggtgcgggtg
     3361 ccagggcgtg cccttgggct ccccgggcgc gtactccact ccccccatgg tgcggtgcgg
     3421 gtgccagggc gtgcccttgg gctccccggg cgcgtactcc actccccgag ccggtgcggg
     3481 tgccagggcg tgcccttggg ctccccgggc gcgtactcca ctccccttcg caagaccctt
     3541 cctctatata aggaagttca tttcatttgg agaggactcc ggtattttta caacaattac
     3601 cacaacaaaa caaacaacaa acaacattac aatttactat tctagtcgaa tactgtattt
     3661 ttacaacaat taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta
     3721 caatggatta ccttaccata atattaactt tactatttgc cttgactctc tatgaagcct
     3781 tcagctacct atccagaaga accaaaaacc ttcctccagg accatcgcca ttgccgttca
     3841 tcggaagcct ccatttatta ggcgaccaac cacacaaatc cttagcaaaa ctttccaaaa
     3901 aacacggtcc aattatgagt ctcaaattag gccagatcac tacaatcgtc atatcttcat
     3961 caacaatggc gaaagaagtt cttcaaaaac aggatttagc attttcaagc agatcagttc
     4021 caaacgcact ccacgctcac aatcaattca aattctccgt tgtatggctt ccggtagcct
     4081 cacgatggag aagccttcga aaagttttga attctaatat attttccggc aatcggctcg
     4141 acgctaatca acatttgaga actagaaaag tacaggaact aattgcgtat tgccggaaaa
     4201 atagccagag cggagaagcg gttgacgtcg gccgagctgc ttttagaact tcgttgaatt
     4261 tgttgtcgaa tttgattttt tcaaaggatt tgacggatcc ttattcggat tctgccaagg
     4321 aattcaagga tttggtttgg aatataatgg ttgaggcggg gaaacctaat ttggtcgatt
     4381 tttttcccct gcttgaaaaa gttgatcctc aaggtatacg acatcgtatg acgattcact
     4441 ttggggaagt tcttaagctt tttggtggac ttgttaatga aagattggag caaagaagat
     4501 caaaagggga aaaaaatgat gtgttggatg tacttctaac taccagccaa gaaagccctg
     4561 aggaaatcga tagaactcac attgagcgaa tgtgcttgga cctgtttgta gcagggacgg
     4621 acacaacatc aagcacatta gaatgggcaa tgtcagaaat gcttaaaaac ccagacaaaa
     4681 tgaagaaaac ccaagatgaa cttgcacaag taatcggcag aggaaaaaca atagaagaat
     4741 ccgatattaa ccgcttacct tacttaagat gcgttatgaa agaaacctta aggatacatc
     4801 caccagttcc cttcttaatt cctcgcaaag tggaacaaag tgttgaggtt tgtggataca
     4861 atgtccctaa aggatcacaa gttcttgtga atgcttgggc aattggacgt gatgaaactg
     4921 tttgggatga tgctttggca ttcaaacccg agagatttat ggaatctgaa ttggatatcc
     4981 gtggaagaga tttcgagctg attccgttcg gtgctggccg aagaatttgc ccagggttgc
     5041 cattggcact aaggactgtg cctttgatgc ttggttcttt gttgaactct tttaattgga
     5101 agcttgaagg tgggatggct ccaaaagatt tggatatgga ggagaagttt ggtattacac
     5161 tgcagaaggc tcatcctttg cgtgctgtac caagcaccct ttaagcttgt cctgctttaa
     5221 tgagatatgc gagaagccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt
     5281 gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg
     5341 aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca atttactgat
     5401 tgtaccctac tacttatatg tacaatatta aaatgaaaac aatatattgt gctgaatagg
     5461 tttatagcga catctatgat agagcgccac aataacaaac aattgcgttt tattattaca
     5521 aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat tacataaatc
     5581 ttattcaaat ttcaaaagtg ccccaggggc tagtatctac gacacaccga gcggcgaact
     5641 aataacgctc actgaaggga actccggttc cccgccggcg cgcatgggtg agattccttg
     5701 aagttgagta ttggccgtcc gctctaccga aagttacggg caccattcaa cccggtccag
     5761 cacggcggcc gggtaaccga cttgctgccc cgagaattat gcagcatttt tttggtgtat
     5821 gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt
     5881 ccagggcgaa ttttgcgaca acatgtcgag gctcagcagg accgctttac gaattcccat
     5941 ggggagcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccactccc
     6001 cgagccggtg cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccactcccc
     6061 ccatggtgcg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccactc
     6121 cccgagccgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccactcc
     6181 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gactccggta
     6241 tttttacaac aattaccaca acaaaacaaa caacaaacaa cattacaatt tactattcta
     6301 gtcgaatact gtatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa
     6361 ttactattta caattacaat gaccaagacc aattcccctg ctccttctgt catcacttgc
     6421 aaagctgctg tggtatggaa atcaggggag ccaccaaagg tggaagagat acaagttgat
     6481 ccaccaaagg cctcagaagt taggattaag atgctttgtg ccagtttgtg ccacactgat
     6541 ttccttgcct gcaatggcct tcctgttcca ttgttccctc gcattcctgg acacgaagga
     6601 gtcggaatga tcgagagcgt tggagaaaat gttacgaacc taaaagaagg agacatagtg
     6661 atgccacttt acttgggaga atgtggggaa tgcttgaatt gcaaatcagg aaggacaaat
     6721 ttgtgccaca aatatccttt aggttttagt ggattattgc ttgatggaac atcaagaatg
     6781 tcaattggag agcaaaaagt atatcaccat ttcagctgtt cgacatggtc agagtatata
     6841 gtgattgaag cagcttatgc agtgaaggta gatccaaggg tttctcttcc acatgctagc
     6901 tttctttgct gtggattcac cactggtttt ggtgctactt ggagagatgt caatgttgtc
     6961 aagggctcta ctgtggctgt tctaggcctt ggtgctgttg gacttggggc tgtgcaagga
     7021 gctaaatcac aaggagcatc aagaattata ggtttggata tcaacgacaa gaaacgtgaa
     7081 aagggagaag catttggaat gactgaattt ataaatccaa aaggttcaaa caaatcaatt
     7141 tctgaactaa taaatgaagc aactggtgga ttaggacttg actatgtcta cgaatgcact
     7201 ggagttccag ctttactcaa tgaagccatt gaatcctcta aagtgggact tggaactgca
     7261 gtattgattg gggcaggact tgaaacaagt ggagaaatca aattcattcc tcttctgtgt
     7321 ggtagaactg ttaaaggttc aatttatggt ggagtaagac ctaaatcaga cctccccact
     7381 ttaattgaaa agtgcataaa caaggaaatt ccaatggatg aactaatgac tcatgaagtt
     7441 tcattgtctg agataaacaa aggatttgag taccttaagc atcctgattg tgtcaaagtt
     7501 gttatcaagt tctaagcttg atcccccgtc gacagctagc tatatcatca atttatgtat
     7561 tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga tataatcagt
     7621 tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg cttggactat
     7681 aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc aagatcgctc
     7741 agagaattcg catgcggagc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc
     7801 gtactccact ccccgagccg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg
     7861 tactccactc cccccatggt gcggtgcggg tgccagggcg tgcccttggg ctccccgggc
     7921 gcgtactcca ctccccgagc cggtgcgggt gccagggcgt gcccttgggc tccccgggcg
     7981 cgtactccac tccccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
     8041 gaggactccg gtatttttac aacaattacc acaacaaaac aaacaacaaa caacattaca
     8101 atttactatt ctagtcgaat actgtatttt tacaacaatt accaacaaca acaaacaaca
     8161 aacaacatta caattactat ttacaattac aatggcgacc atcactttcg attcactaaa
     8221 cccggtgacc gtcgccatct cagccggttt tcttctcctc cttatcatct ttgtgaaatc
     8281 aagaactggt tcatccaaaa gaaaaccgcc gggtcctcca ggatggccga ttttcggcaa
     8341 tatgttcgac ctcggagatt tacctcacca aacgctgtac aagctcaagt ccaaatacgg
     8401 gccgatcgta tggctccagc tcggctcaat taatactatg gttgttcaaa atgcagtttc
     8461 cgccgccgag ctttttaaga aacacgatgt ccccttttgt gaccgaaaag tccctgatac
     8521 tctcactgcc ttcaatttca accagggctc cctgggtatg aatacttacg gcggacattg
     8581 gcgagtgctt agacgcctct gttccatgga gtttttggta aataaacgta tgaacgaaac
     8641 tacagatcta agaaggagaa ttgaggacaa tatggtccgt tggattgaag aagattcatt
     8701 ggcatctaaa gcacaaggag gaaccggagc ggttcaattg agcaggtttc tttttctaat
     8761 ggcgtttaat ttggtgggta accttatgtt atcgagagat ttaatggata ataaggatcc
     8821 agaaggaagg gaattttttg attgtatgaa tgagatattg gaattggctg gtactcctaa
     8881 tattgctgat tttttgcctt tgttgaagaa attggatcca cttggaatga agaaaaggat
     8941 ggttgataat atgtctagaa ctatgaaaat ttcttcaaag tttgttcaag aaagacttga
     9001 taatagaaaa gctggaaaga ttaatgaaaa gaaagatttc ttggatgtta tgcttgaata
     9061 ccaaggtgat ggtaaagatg gtcccgataa attcactgag caacatgtca acattgtcat
     9121 catggaaatg ttttttgcag gatcagagac aacaagtatc agcattgaat ggggattcac
     9181 agaacttctc cgaaacccac acgcattcaa gaaagtaagg gaagagatcg acagagtagt
     9241 gggagtaaac agaatggtcg aagagagcga catggagaat ttgccgtatt tgcaagcagt
     9301 tgtaaaggaa acgcttagat tgcatccggc acttccaatg cttctaccaa gaaacacaat
     9361 ggaggacact gaatacatgg gatacctcat tcctaaaggc acacaagttt tcgtgaatgc
     9421 atgggcaatc ggaagggatc ctgagtactg gcaggaccct ttgagtttca aaccagaaag
     9481 gtttatcaat tccagtgttg aatataaagg acaacatttt gaattgattc cttttggttc
     9541 tggaagaaga atttgtgtgg gatttccttt ggctcataga gttgttcatc tcactttggc
     9601 tacattagtc caggcttttg attgggatct tggtgctggc gttaaaccac aagatattga
     9661 tttggaagag agattgggat tgactctgag gaagaagaat ccattgaatg tcatccctaa
     9721 gaagagagtt catatctaag cttctctagc tagagtcgat cgacaagctc gagtttctcc
     9781 ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca
     9841 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     9901 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatcgc ttgtggaatt
     9961 cctcgaggga gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca
    10021 ctccccgagc cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
    10081 tccccccatg gtgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc
    10141 cactccccga gccggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
    10201 actccccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggactc
    10261 cggtattttt acaacaatta ccacaacaaa acaaacaaca aacaacatta caatttacta
    10321 ttctagtcga atactgtatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat
    10381 tacaattact atttacaatt acaatgggtt ctcaagaaac aaatttggct cctcatgttc
    10441 tcatattccc tttgccaatt caaggccatg tgaactccat gctcagacta gcagaacttc
    10501 tctgtctagc tgaattagat atcactttta ttgtttcaga gttcagccac agtcgcttaa
    10561 ttaagcatac aaatgttgcc tcccgttttg cccgttaccc aggatttcag ttccagccca
    10621 tatccgatgg ccttcccgat gatcacccca gagcaggcga aagagtcatg gatattttgc
    10681 cttcaactaa aaatgtaacg ggacccctgt ttaagcagat gatggttgag aataagtgct
    10741 tttcttctgc tactcgaagg ccgattactt gcataatcgc tgatggggtt ttgagctttg
    10801 ctggggattt cgctcaagaa aaaggaatcc ctcttattta cttcagaact gtttctgctt
    10861 gttctttctg ggcttgtttt tgtatgcctg aactaattga atctggtgac attccgatta
    10921 aaggaaatgg tatggatctg atagtgaaaa gcgtgccggg gatggaaact tttctcaggc
    10981 gacgagatct gccggggttt tgccgagtca atgacattaa tgaaccgaaa ctccagatcc
    11041 tgaaaacaga gacaagacag acaacgagag cccaagcggc tattttgaac acttttgagg
    11101 atttggaagg accaattcta tctcaaattc gcaaacacat gccaagactt ttcacaattg
    11161 gaccaagtca ttcccaccta acatctagac ttgaaaccaa gaatatcaag actttaattt
    11221 cctccggtag tttctgggaa gaagatcgaa gctgtgtgga ttggcttgat gcacagccac
    11281 caagatctgt tttatatgta agttttggaa gtattacagt tgtaacaaga gatcaactcc
    11341 tggaattctg gtatggtctt gttaatagtg gacaacggtt cttgtgggta atgaggccag
    11401 attcaattat gggcaaagat gggcaaagcc agattcctgc agatcttgaa gagggcacaa
    11461 aagccagagg ttatatggtg ggatgggcac ctcaagaaga ggtcttgaat caccctgcca
    11521 ttggaggatt tctgactcac agtgggtgga attcaacttt agagagtatt gtggctggtg
    11581 tcccaatgat atgttggcca tactttgcgg accaaatgat aaacagcaga tttgtgagtg
    11641 aaatttggaa gattggattg gatatgaaag atacatgtga tagagagaca attgtgaaga
    11701 tggttaggga gttgatggag attaggaaag atgagttctt gcaaagggct gatcatatgg
    11761 ctaaattggc aaaagaggct gttagtgaag gcggatcctc atattctaac ttggatggcc
    11821 tcgttgatta cataaaatca ctgattattt gagcttgtca agcagatcgt tcaaacattt
    11881 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
    11941 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
    12001 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
    12061 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgac
    12121 gctgagcttg tcttcgagga tgcacatgtg accgagggac acgaagtgat ccgtttaaac
    12181 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
    12241 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
    12301 gccaaccaca gggttcccct cgggagtcag ccgtgcggct gcatgaaatc ctggccggtt
    12361 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
    12421 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
    12481 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
    12541 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
    12601 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
    12661 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
    12721 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
    12781 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
    12841 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
    12901 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
    12961 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
    13021 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
    13081 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
    13141 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
    13201 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
    13261 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
    13321 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
    13381 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
    13441 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
    13501 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
    13561 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
    13621 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
    13681 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
    13741 aaagaatccc ggcaaccgcc ggcagccggt gcgtcgtcga ttaggaagcc gcccaagggc
    13801 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
    13861 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
    13921 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
    13981 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
    14041 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
    14101 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
    14161 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
    14221 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
    14281 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
    14341 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
    14401 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
    14461 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
    14521 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
    14581 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
    14641 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
    14701 ggtcgaaaaa gcttctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
    14761 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
    14821 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
    14881 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
    14941 cagctgcaaa aagcgcccac atcaaggctc cgagtgcgcg gaacccctat ttgtttattt
    15001 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    15061 taatattgaa aaaggaagag tatggctaaa atgagaatat caccggaatt gaaaaaactg
    15121 atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag
    15181 ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc
    15241 acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt
    15301 ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc
    15361 gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag
    15421 ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg
    15481 aatagcttag acagccgctt agccgaattg gattacttac tgaataacga tctggccgat
    15541 gtggattgcg aaaactggga agaggacact ccatttaaag atccgcgcga gctgtatgat
    15601 tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac
    15661 agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc
    15721 agggcggaca agtggtatga cattgccttc tgcgtccggt cgctcaggga ggatatcggg
    15781 gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa
    15841 ataaaatatt atattttact ggatgaattg ttttagctgt cagaccaagt ttactcatat
    15901 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    15961 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    16021 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    16081 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    16141 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    16201 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    16261 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    16321 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    16381 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    16441 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    16501 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    16561 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    16621 cggagcc
//
