LOCUS       sequence-349580-       16609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 2 plasmid containing multiple transcriptional units for 
            transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and 
            CrG8H driven by 4x opaatB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 16609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD2KN0897
FEATURES             Location/Qualifiers
     source          1..16609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    125..149
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    246..279
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    305..338
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    368..401
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    427..460
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        477..523
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    613..668
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     polyA_signal    1871..2045
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    2077..2110
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2136..2169
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2199..2232
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2258..2291
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        2308..2354
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    2444..2499
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      2985..3237
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     protein_bind    3270..3303
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    3329..3362
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    3392..3425
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    3451..3484
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        3501..3547
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    3637..3692
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      5155..5407
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    5442..5475
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    5501..5534
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    5564..5597
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    5623..5656
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        5673..5719
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    5809..5864
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      7022..7222
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    7255..7288
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    7314..7347
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    7377..7410
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    7436..7469
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        7486..7532
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    7622..7677
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     polyA_signal    9261..9435
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    9467..9500
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    9526..9559
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    9589..9622
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    9648..9681
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        9698..9744
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    9834..9889
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      11380..12087
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    12154..12178
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12487..13116
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13545..14618
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14684..14878
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        14940..15044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             15045..15839
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      16010..16598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16499..16518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcggccttt ttacggttcc tgctcggatc tgttggaccg gacagtagtc atggttgatg
       61 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
      121 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
      181 tgcggacgtt tttaatgtac tggggttgaa cactctgtgc cgaattcgga tccggagcgg
      241 tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccactcc ccgagccggt
      301 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cccatggtgc
      361 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccact ccccgagccg
      421 gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccactc cccttcgcaa
      481 gacccttcct ctatataagg aagttcattt catttggaga ggactccggt atttttacaa
      541 caattaccac aacaaaacaa acaacaaaca acattacaat ttactattct agtcgaatac
      601 tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca attactattt
      661 acaattacaa tgagttggtg gtggaagagg tccattggtg ctggaaagaa cttaccaaac
      721 cagaacaaag aaaatggagt ttgtaaaagc tacaagagtg tggcactagt agtaggagtc
      781 accggcattg ttggcagtag tttagccgaa gttctaaagc taccagacac ccctggaggt
      841 ccctggaagg tatacggcgt ggcaaggcgc ccatgccctg tatggctagc caaaaaacca
      901 gttgagtaca tccaatgtga tgtctcagat aatcaagaaa caatttctaa attatctcca
      961 cttaaggaca taactcatat attctatgtt tcttggattg gatcagaaga ttgccaaaca
     1021 aatgctacaa tgttcaaaaa catccttaat tcagtgattc ctaatgcttc aaatctccaa
     1081 catgtgtgtc tccaaacagg aatcaaacat tattttggta tttttgaaga aggttctaaa
     1141 gttgtaccac atgattcgcc ttttaccgag gatttaccta ggctaaatgt cccaaatttc
     1201 taccatgatc ttgaagatat actgtatgaa gaaactggca agaacaattt aacatggtct
     1261 gtccatagac cagctctggt tttcgggttt tccccatgta gtatgatgaa cattgtcagt
     1321 acattatgcg tttacgccac aatttgtaaa catgaaaata aggccttggt ttatccaggt
     1381 agtaaaaatt catggaattg ttatgctgat gctgttgatg cagatttagt agccgagcac
     1441 gaaatttggg cagcagttga tcctaaggct aaaaatcaag tattgaattg taacaatggg
     1501 gatgttttta aatggaaaca tatttggaag aaattagcag aggaatttgg gattgaaatg
     1561 gtgggatatg ttgaaggtaa agaacaagtt agtttggctg agttaatgaa ggataaagat
     1621 caagtttggg atgaaattgt taagaagaac aatttagtac ctactaaatt gaaagaaatt
     1681 gctgcctttt ggtttgctga tattgctttt tgttctgaga atttgattag tagtatgaac
     1741 aaaagtaagg aattagggtt cttgggtttt aggaattcga tgaaatcgtt tgtttcttgt
     1801 attgataaga tgagggatta taggtttatt ccttaggctt ctctagctag agtcgatcga
     1861 caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga attagggttc
     1921 ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     1981 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc
     2041 agatcgctgc aagaattcaa gcttggagcg gtgcgggtgc cagggcgtgc ccttgggctc
     2101 cccgggcgcg tactccactc cccgagccgg tgcgggtgcc agggcgtgcc cttgggctcc
     2161 ccgggcgcgt actccactcc ccccatggtg cggtgcgggt gccagggcgt gcccttgggc
     2221 tccccgggcg cgtactccac tccccgagcc ggtgcgggtg ccagggcgtg cccttgggct
     2281 ccccgggcgc gtactccact ccccttcgca agacccttcc tctatataag gaagttcatt
     2341 tcatttggag aggactccgg tatttttaca acaattacca caacaaaaca aacaacaaac
     2401 aacattacaa tttactattc tagtcgaata ctgtattttt acaacaatta ccaacaacaa
     2461 caaacaacaa acaacattac aattactatt tacaattaca atggcttcga agcttgaaat
     2521 agaaattgag ttgaaaagtg atgtagaaaa gatgtggaaa cacttcaaag aattcaccaa
     2581 attattcccc aaggcttgtc ctcatcttta cgagaagatt gatgtgatcg agggagatgg
     2641 gatatcagtt ggaacaatct ttgtcagtac cctgaaacca acagagttga accccgtggt
     2701 tatggtgacg aaggagaaga ttgatttttt agatgatgag aacaaaatgt tgaggtatag
     2761 ttacatggaa ggggagatat taaagaatta caagaatttg aggggcacag tgcatatgag
     2821 cagcagcaaa agtggtggaa ctatattcaa atattcagga gaattcgaaa aggcaaatga
     2881 acaagtccct gatcctgttt tcttcaaaga ttttatggtc atagttttcc aaggtttgga
     2941 tgattatatt ctcaagggca tgaaccatac atgtcaaaat taagcttgga ctcccatgtt
     3001 ggcaaaggca accaaacaaa caatgaatga tccgctcctg catatggggc ggtttgagta
     3061 tttcaactgc catttgggct gaattgtaga catgctcctg tcagaaattc cgtgatctta
     3121 ctcaatattc agtaatctcg gccaatatcc taaatgtgcg tggctttatc tgtctttgta
     3181 ttgtttcatc aattcatgta acgtttgctt ttcttatgaa ttttcaaata aattatccgc
     3241 tactagaatt cgagctcgga gcggtgcggg tgccagggcg tgcccttggg ctccccgggc
     3301 gcgtactcca ctccccgagc cggtgcgggt gccagggcgt gcccttgggc tccccgggcg
     3361 cgtactccac tccccccatg gtgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg
     3421 gcgcgtactc cactccccga gccggtgcgg gtgccagggc gtgcccttgg gctccccggg
     3481 cgcgtactcc actccccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     3541 gagaggactc cggtattttt acaacaatta ccacaacaaa acaaacaaca aacaacatta
     3601 caatttacta ttctagtcga atactgtatt tttacaacaa ttaccaacaa caacaaacaa
     3661 caaacaacat tacaattact atttacaatt acaatgggtt ctcaagaaac aaatttggct
     3721 cctcatgttc tcatattccc tttgccaatt caaggccatg tgaactccat gctcagacta
     3781 gcagaacttc tctgtctagc tgaattagat atcactttta ttgtttcaga gttcagccac
     3841 agtcgcttaa ttaagcatac aaatgttgcc tcccgttttg cccgttaccc aggatttcag
     3901 ttccagccca tatccgatgg ccttcccgat gatcacccca gagcaggcga aagagtcatg
     3961 gatattttgc cttcaactaa aaatgtaacg ggacccctgt ttaagcagat gatggttgag
     4021 aataagtgct tttcttctgc tactcgaagg ccgattactt gcataatcgc tgatggggtt
     4081 ttgagctttg ctggggattt cgctcaagaa aaaggaatcc ctcttattta cttcagaact
     4141 gtttctgctt gttctttctg ggcttgtttt tgtatgcctg aactaattga atctggtgac
     4201 attccgatta aaggaaatgg tatggatctg atagtgaaaa gcgtgccggg gatggaaact
     4261 tttctcaggc gacgagatct gccggggttt tgccgagtca atgacattaa tgaaccgaaa
     4321 ctccagatcc tgaaaacaga gacaagacag acaacgagag cccaagcggc tattttgaac
     4381 acttttgagg atttggaagg accaattcta tctcaaattc gcaaacacat gccaagactt
     4441 ttcacaattg gaccaagtca ttcccaccta acatctagac ttgaaaccaa gaatatcaag
     4501 actttaattt cctccggtag tttctgggaa gaagatcgaa gctgtgtgga ttggcttgat
     4561 gcacagccac caagatctgt tttatatgta agttttggaa gtattacagt tgtaacaaga
     4621 gatcaactcc tggaattctg gtatggtctt gttaatagtg gacaacggtt cttgtgggta
     4681 atgaggccag attcaattat gggcaaagat gggcaaagcc agattcctgc agatcttgaa
     4741 gagggcacaa aagccagagg ttatatggtg ggatgggcac ctcaagaaga ggtcttgaat
     4801 caccctgcca ttggaggatt tctgactcac agtgggtgga attcaacttt agagagtatt
     4861 gtggctggtg tcccaatgat atgttggcca tactttgcgg accaaatgat aaacagcaga
     4921 tttgtgagtg aaatttggaa gattggattg gatatgaaag atacatgtga tagagagaca
     4981 attgtgaaga tggttaggga gttgatggag attaggaaag atgagttctt gcaaagggct
     5041 gatcatatgg ctaaattggc aaaagaggct gttagtgaag gcggatcctc atattctaac
     5101 ttggatggcc tcgttgatta cataaaatca ctgattattt gagcttgtca agcagatcgt
     5161 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     5221 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     5281 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     5341 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     5401 ctagatcgac gctttacgaa ttcccatggg gagcggtgcg ggtgccaggg cgtgcccttg
     5461 ggctccccgg gcgcgtactc cactccccga gccggtgcgg gtgccagggc gtgcccttgg
     5521 gctccccggg cgcgtactcc actcccccca tggtgcggtg cgggtgccag ggcgtgccct
     5581 tgggctcccc gggcgcgtac tccactcccc gagccggtgc gggtgccagg gcgtgccctt
     5641 gggctccccg ggcgcgtact ccactcccct tcgcaagacc cttcctctat ataaggaagt
     5701 tcatttcatt tggagaggac tccggtattt ttacaacaat taccacaaca aaacaaacaa
     5761 caaacaacat tacaatttac tattctagtc gaatactgta tttttacaac aattaccaac
     5821 aacaacaaac aacaaacaac attacaatta ctatttacaa ttacaatgac caagaccaat
     5881 tcccctgctc cttctgtcat cacttgcaaa gctgctgtgg tatggaaatc aggggagcca
     5941 ccaaaggtgg aagagataca agttgatcca ccaaaggcct cagaagttag gattaagatg
     6001 ctttgtgcca gtttgtgcca cactgatttc cttgcctgca atggccttcc tgttccattg
     6061 ttccctcgca ttcctggaca cgaaggagtc ggaatgatcg agagcgttgg agaaaatgtt
     6121 acgaacctaa aagaaggaga catagtgatg ccactttact tgggagaatg tggggaatgc
     6181 ttgaattgca aatcaggaag gacaaatttg tgccacaaat atcctttagg ttttagtgga
     6241 ttattgcttg atggaacatc aagaatgtca attggagagc aaaaagtata tcaccatttc
     6301 agctgttcga catggtcaga gtatatagtg attgaagcag cttatgcagt gaaggtagat
     6361 ccaagggttt ctcttccaca tgctagcttt ctttgctgtg gattcaccac tggttttggt
     6421 gctacttgga gagatgtcaa tgttgtcaag ggctctactg tggctgttct aggccttggt
     6481 gctgttggac ttggggctgt gcaaggagct aaatcacaag gagcatcaag aattataggt
     6541 ttggatatca acgacaagaa acgtgaaaag ggagaagcat ttggaatgac tgaatttata
     6601 aatccaaaag gttcaaacaa atcaatttct gaactaataa atgaagcaac tggtggatta
     6661 ggacttgact atgtctacga atgcactgga gttccagctt tactcaatga agccattgaa
     6721 tcctctaaag tgggacttgg aactgcagta ttgattgggg caggacttga aacaagtgga
     6781 gaaatcaaat tcattcctct tctgtgtggt agaactgtta aaggttcaat ttatggtgga
     6841 gtaagaccta aatcagacct ccccacttta attgaaaagt gcataaacaa ggaaattcca
     6901 atggatgaac taatgactca tgaagtttca ttgtctgaga taaacaaagg atttgagtac
     6961 cttaagcatc ctgattgtgt caaagttgtt atcaagttct aagcttgatc ccccgtcgac
     7021 agctagctat atcatcaatt tatgtattac acataatatc gcactcagtc tttcatctac
     7081 ggcaatgtac cagctgatat aatcagttat tgaaatattt ctgaatttaa acttgcatca
     7141 ataaatttat gtttttgctt ggactataat acctgacttg ttattttatc aataaatatt
     7201 taaactatat ttctttcaag atcgctcaga gaattcgcat gcggagcggt gcgggtgcca
     7261 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cgagccggtg cgggtgccag
     7321 ggcgtgccct tgggctcccc gggcgcgtac tccactcccc ccatggtgcg gtgcgggtgc
     7381 cagggcgtgc ccttgggctc cccgggcgcg tactccactc cccgagccgg tgcgggtgcc
     7441 agggcgtgcc cttgggctcc ccgggcgcgt actccactcc ccttcgcaag acccttcctc
     7501 tatataagga agttcatttc atttggagag gactccggta tttttacaac aattaccaca
     7561 acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaatact gtatttttac
     7621 aacaattacc aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat
     7681 ggcgaccatc actttcgatt cactaaaccc ggtgaccgtc gccatctcag ccggttttct
     7741 tctcctcctt atcatctttg tgaaatcaag aactggttca tccaaaagaa aaccgccggg
     7801 tcctccagga tggccgattt tcggcaatat gttcgacctc ggagatttac ctcaccaaac
     7861 gctgtacaag ctcaagtcca aatacgggcc gatcgtatgg ctccagctcg gctcaattaa
     7921 tactatggtt gttcaaaatg cagtttccgc cgccgagctt tttaagaaac acgatgtccc
     7981 cttttgtgac cgaaaagtcc ctgatactct cactgccttc aatttcaacc agggctccct
     8041 gggtatgaat acttacggcg gacattggcg agtgcttaga cgcctctgtt ccatggagtt
     8101 tttggtaaat aaacgtatga acgaaactac agatctaaga aggagaattg aggacaatat
     8161 ggtccgttgg attgaagaag attcattggc atctaaagca caaggaggaa ccggagcggt
     8221 tcaattgagc aggtttcttt ttctaatggc gtttaatttg gtgggtaacc ttatgttatc
     8281 gagagattta atggataata aggatccaga aggaagggaa ttttttgatt gtatgaatga
     8341 gatattggaa ttggctggta ctcctaatat tgctgatttt ttgcctttgt tgaagaaatt
     8401 ggatccactt ggaatgaaga aaaggatggt tgataatatg tctagaacta tgaaaatttc
     8461 ttcaaagttt gttcaagaaa gacttgataa tagaaaagct ggaaagatta atgaaaagaa
     8521 agatttcttg gatgttatgc ttgaatacca aggtgatggt aaagatggtc ccgataaatt
     8581 cactgagcaa catgtcaaca ttgtcatcat ggaaatgttt tttgcaggat cagagacaac
     8641 aagtatcagc attgaatggg gattcacaga acttctccga aacccacacg cattcaagaa
     8701 agtaagggaa gagatcgaca gagtagtggg agtaaacaga atggtcgaag agagcgacat
     8761 ggagaatttg ccgtatttgc aagcagttgt aaaggaaacg cttagattgc atccggcact
     8821 tccaatgctt ctaccaagaa acacaatgga ggacactgaa tacatgggat acctcattcc
     8881 taaaggcaca caagttttcg tgaatgcatg ggcaatcgga agggatcctg agtactggca
     8941 ggaccctttg agtttcaaac cagaaaggtt tatcaattcc agtgttgaat ataaaggaca
     9001 acattttgaa ttgattcctt ttggttctgg aagaagaatt tgtgtgggat ttcctttggc
     9061 tcatagagtt gttcatctca ctttggctac attagtccag gcttttgatt gggatcttgg
     9121 tgctggcgtt aaaccacaag atattgattt ggaagagaga ttgggattga ctctgaggaa
     9181 gaagaatcca ttgaatgtca tccctaagaa gagagttcat atctaagctt ctctagctag
     9241 agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga
     9301 attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     9361 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     9421 actaaaatcc agatcgcttg tggaattcct cgagggagcg gtgcgggtgc cagggcgtgc
     9481 ccttgggctc cccgggcgcg tactccactc cccgagccgg tgcgggtgcc agggcgtgcc
     9541 cttgggctcc ccgggcgcgt actccactcc ccccatggtg cggtgcgggt gccagggcgt
     9601 gcccttgggc tccccgggcg cgtactccac tccccgagcc ggtgcgggtg ccagggcgtg
     9661 cccttgggct ccccgggcgc gtactccact ccccttcgca agacccttcc tctatataag
     9721 gaagttcatt tcatttggag aggactccgg tatttttaca acaattacca caacaaaaca
     9781 aacaacaaac aacattacaa tttactattc tagtcgaata ctgtattttt acaacaatta
     9841 ccaacaacaa caaacaacaa acaacattac aattactatt tacaattaca atggattacc
     9901 ttaccataat attaacttta ctatttgcct tgactctcta tgaagccttc agctacctat
     9961 ccagaagaac caaaaacctt cctccaggac catcgccatt gccgttcatc ggaagcctcc
    10021 atttattagg cgaccaacca cacaaatcct tagcaaaact ttccaaaaaa cacggtccaa
    10081 ttatgagtct caaattaggc cagatcacta caatcgtcat atcttcatca acaatggcga
    10141 aagaagttct tcaaaaacag gatttagcat tttcaagcag atcagttcca aacgcactcc
    10201 acgctcacaa tcaattcaaa ttctccgttg tatggcttcc ggtagcctca cgatggagaa
    10261 gccttcgaaa agttttgaat tctaatatat tttccggcaa tcggctcgac gctaatcaac
    10321 atttgagaac tagaaaagta caggaactaa ttgcgtattg ccggaaaaat agccagagcg
    10381 gagaagcggt tgacgtcggc cgagctgctt ttagaacttc gttgaatttg ttgtcgaatt
    10441 tgattttttc aaaggatttg acggatcctt attcggattc tgccaaggaa ttcaaggatt
    10501 tggtttggaa tataatggtt gaggcgggga aacctaattt ggtcgatttt tttcccctgc
    10561 ttgaaaaagt tgatcctcaa ggtatacgac atcgtatgac gattcacttt ggggaagttc
    10621 ttaagctttt tggtggactt gttaatgaaa gattggagca aagaagatca aaaggggaaa
    10681 aaaatgatgt gttggatgta cttctaacta ccagccaaga aagccctgag gaaatcgata
    10741 gaactcacat tgagcgaatg tgcttggacc tgtttgtagc agggacggac acaacatcaa
    10801 gcacattaga atgggcaatg tcagaaatgc ttaaaaaccc agacaaaatg aagaaaaccc
    10861 aagatgaact tgcacaagta atcggcagag gaaaaacaat agaagaatcc gatattaacc
    10921 gcttacctta cttaagatgc gttatgaaag aaaccttaag gatacatcca ccagttccct
    10981 tcttaattcc tcgcaaagtg gaacaaagtg ttgaggtttg tggatacaat gtccctaaag
    11041 gatcacaagt tcttgtgaat gcttgggcaa ttggacgtga tgaaactgtt tgggatgatg
    11101 ctttggcatt caaacccgag agatttatgg aatctgaatt ggatatccgt ggaagagatt
    11161 tcgagctgat tccgttcggt gctggccgaa gaatttgccc agggttgcca ttggcactaa
    11221 ggactgtgcc tttgatgctt ggttctttgt tgaactcttt taattggaag cttgaaggtg
    11281 ggatggctcc aaaagatttg gatatggagg agaagtttgg tattacactg cagaaggctc
    11341 atcctttgcg tgctgtacca agcacccttt aagcttgtcc tgctttaatg agatatgcga
    11401 gaagcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg
    11461 agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc
    11521 gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg taccctacta
    11581 cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca
    11641 tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta
    11701 aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt
    11761 caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac
    11821 tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt
    11881 ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg
    11941 gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa
    12001 tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt
    12061 ttgcgacaac atgtcgaggc tcagcaggac cgctgagcga ggatgcacat gtgaccgagg
    12121 gacacgaagt gatccgttta aactatcagt gtttgacagg atatattggc gggtaaacct
    12181 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
    12241 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggagt cagccgtgcg
    12301 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
    12361 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
    12421 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
    12481 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
    12541 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
    12601 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
    12661 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
    12721 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
    12781 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
    12841 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
    12901 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
    12961 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
    13021 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
    13081 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
    13141 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
    13201 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
    13261 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
    13321 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
    13381 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
    13441 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
    13501 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
    13561 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
    13621 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
    13681 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgtcgt
    13741 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
    13801 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
    13861 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
    13921 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
    13981 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
    14041 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
    14101 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
    14161 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
    14221 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
    14281 ctgattggat gtaccgcgag attacagaag gcaagaaccc ggacgtgctg acggttcacc
    14341 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
    14401 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
    14461 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
    14521 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
    14581 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
    14641 aaattgccct agcaggggaa aaaggtcgaa aaagcttctt tcctgtggat agcacgtaca
    14701 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
    14761 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
    14821 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
    14881 tgtctggcca gcgcacagcc gaacagctgc aaaaagcgcc cacatcaagg ctccgagtgc
    14941 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    15001 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa
    15061 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
    15121 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
    15181 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
    15241 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
    15301 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
    15361 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
    15421 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
    15481 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta
    15541 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
    15601 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
    15661 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
    15721 ggtcgctcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
    15781 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc
    15841 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    15901 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    15961 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    16021 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    16081 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    16141 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    16201 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    16261 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    16321 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    16381 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    16441 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    16501 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    16561 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaa
//
