LOCUS       sequence-349581-       15507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 2 plasmid containing multiple transcriptional units for 
            transient expression of CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS 
            driven by 4x opaatB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15507)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 15507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD2KN0895
FEATURES             Location/Qualifiers
     source          1..15507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    132..156
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    253..286
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    312..345
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    375..408
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    434..467
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        484..530
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    620..675
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     polyA_signal    1770..1944
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    1976..2009
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2035..2068
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2098..2131
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2157..2190
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        2207..2253
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    2343..2398
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      3967..4219
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     protein_bind    4252..4285
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    4311..4344
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    4374..4407
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    4433..4466
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        4483..4529
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    4619..4674
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      5799..6506
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    6542..6575
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6601..6634
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6664..6697
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6723..6756
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        6773..6819
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    6909..6964
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      8488..8688
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    8721..8754
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    8780..8813
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    8843..8876
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    8902..8935
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        8952..8998
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    9088..9143
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      10741..10993
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11059..11083
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11392..12021
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12450..13523
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13589..13783
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        13845..13949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             13950..14744
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14915..15503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15404..15423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccagcaacgc ggccttttta cggttcctgc tcggatctgt tggaccggac agtagtcatg
       61 gttgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
      121 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
      181 aacacattgc ggacgttttt aatgtactgg ggttgaacac tctgtgccga attcggatcc
      241 ggagcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccactccccg
      301 agccggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cactcccccc
      361 atggtgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccactccc
      421 cgagccggtg cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccactcccc
      481 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt
      541 tttacaacaa ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt
      601 cgaatactgt atttttacaa caattaccaa caacaacaaa caacaaacaa cattacaatt
      661 actatttaca attacaatgg caaacttttc tgaatctaaa tccatgatgg cagttttctt
      721 catgtttttc ctgcttcttt tgtcatcttc tagttcttca tcttcaagtt caccaatttt
      781 gaaaaagatt tttattgaaa gcccttccta tgctccgaat gccttcacct tcgattcaac
      841 tgataaaggg ttctacactt ccgtccaaga tggccgagtt atcaaatatg aagggccaaa
      901 ttcaggcttc actgacttcg cctacgcatc tcccttctgg aacaaagctt tttgtgagaa
      961 cagcaccgat ccagagaaaa gaccattgtg tgggaggaca tatgatattt cctatgacta
     1021 taagaacagc caaatgtaca ttgttgatgg ccattaccat ctttgtgtgg ttggaaaaga
     1081 aggtgggtat gccacacaac tagccacaag tgtgcaagga gtgccattca aatggctcta
     1141 tgcagtaact gttgatcaga gaacagggat tgtttatttc actgatgtta gctccataca
     1201 tgatgacagt cccgaaggtg tggaagaaat catgaataca agtgatagaa cagggagatt
     1261 aatgaagtat gatccttcaa caaaagaaac caccttatta ttgaaagagc tacatgttcc
     1321 cggcggtgca gaaatcagcg cagatggttc ctttgttgta gtagcagaat ttttaagcaa
     1381 tcggatagtg aagtattggc tagaagggcc aaagaaaggc agtgcagagt tcttagttac
     1441 aatcccaaat ccaggaaata taaagaggaa ttctgatggc catttttggg tgtcatctag
     1501 tgaagaatta gatggaggtc aacatggaag agttgtttca agaggaatta agtttgatgg
     1561 atttgggaat attcttcaag ttataccact tccaccacca tatgaaggtg aacattttga
     1621 acagattcaa gagcacgatg gtttgttata cattggaagt ctcttccata gctctgtggg
     1681 tatattagtg tatgatgatc atgataacaa gggaaattct tatgtttcta gctgagcttc
     1741 tctagctaga gtcgatcgac aagctcgagt ttctccataa taatgtgtga gtagttccca
     1801 gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     1861 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     1921 aaatccagta ctaaaatcca gatcgctgca agaattcaag cttggagcgg tgcgggtgcc
     1981 agggcgtgcc cttgggctcc ccgggcgcgt actccactcc ccgagccggt gcgggtgcca
     2041 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cccatggtgc ggtgcgggtg
     2101 ccagggcgtg cccttgggct ccccgggcgc gtactccact ccccgagccg gtgcgggtgc
     2161 cagggcgtgc ccttgggctc cccgggcgcg tactccactc cccttcgcaa gacccttcct
     2221 ctatataagg aagttcattt catttggaga ggactccggt atttttacaa caattaccac
     2281 aacaaaacaa acaacaaaca acattacaat ttactattct agtcgaatac tgtattttta
     2341 caacaattac caacaacaac aaacaacaaa caacattaca attactattt acaattacaa
     2401 tggaattgaa cttcaaatca attattttct tggtttttgt tagtctaacc ctttactggg
     2461 tctatagaat cttggattgg gtatggttta agcccaagaa gctggaaaaa tgcttaagag
     2521 aacaagggtt taagggaaat ccttacagat tgttcttggg agatcagtat gatagtggaa
     2581 aattgatcag acaagccttg actaaaccta ttggtgttga agaagatgtt aagaaacgaa
     2641 ttgtacctca tatactcaaa actgttggga ctcatggcaa gaagtctttt atgtgggttg
     2701 gaagaatacc aagggtgaat atcacagatc cagagctgat taaagaggtc ttaacaaaat
     2761 actataagtt ccagaagaat catcatgatc ttgacccaat taccaaattg cttttgactg
     2821 gaattggaag cttagaaggt gacccttggg ctaaacgccg aaaaatcatc aatgctgctt
     2881 ttcactttga aaagttaaag cttatgcttc ctgcattcta tttgagttgc cgtgatatgg
     2941 tgacaaaatg ggataataag gtccctgaag gaggatcagc agaggtggat gtgtggcatg
     3001 atattgaaac cttaactgga gatgtaattt caagaacatt atttggaagt aacttcgagg
     3061 aagggagaag aatatttgaa ctcatgaaag aacttactgc tcttactatt gatgtcattc
     3121 gctcggttta tatccctgga cagaggtttc ttccaactaa gaggaacaat aggatgagag
     3181 caattgacaa agaagttaga gtaagaataa cagaaattat caataagaaa atgaaggtaa
     3241 tgaaatcagg agaagcagca agtgctgctg atgatttttt gggaatattg ttggaatgca
     3301 atctgaatga aataaaggag caaggaaata acaaaagtgc tggaatgact attggagaga
     3361 ttattggtga gtgcaaactc ttctattttg ctggccagga cactacctcg actttactcg
     3421 tgtggacaat ggtcctcttg tctagattcc ccgaatggca aactcgtgcc agagaagaag
     3481 tttttcaagt ctttggtaat aaaaccccgg actatgatgg tatcagccat cttaaagtta
     3541 taacgatgat cttgtacgag gttttgaggt tatatactcc agtggctgaa ctgacaaaag
     3601 tggctcatga agctacacaa ctgggaaaat acttcattcc agcgggcgtg cagctaatga
     3661 tgccacaaat cctacttcat catgaccctg aaatatgggg tgaagatgta atggagttca
     3721 aaccagaaag atttgcagag ggagttctca aggcaactaa gagccaaggc tctttttttc
     3781 catttagctt gggaccaaga atgtgcattg gccaaaactt tgctctattg gaagccaaaa
     3841 tggcaatgtc tctaatcttg cgtagatttt ctttcgagct ttctccctcc tatgttcatg
     3901 ctcctttcac tctcatcaca atgcaacccc aatatggtgc tcatttgatt ctgcacaaac
     3961 tctaagcttg gactcccatg ttggcaaagg caaccaaaca aacaatgaat gatccgctcc
     4021 tgcatatggg gcggtttgag tatttcaact gccatttggg ctgaattgta gacatgctcc
     4081 tgtcagaaat tccgtgatct tactcaatat tcagtaatct cggccaatat cctaaatgtg
     4141 cgtggcttta tctgtctttg tattgtttca tcaattcatg taacgtttgc ttttcttatg
     4201 aattttcaaa taaattatcc gctactagaa ttcgagctcg gagcggtgcg ggtgccaggg
     4261 cgtgcccttg ggctccccgg gcgcgtactc cactccccga gccggtgcgg gtgccagggc
     4321 gtgcccttgg gctccccggg cgcgtactcc actcccccca tggtgcggtg cgggtgccag
     4381 ggcgtgccct tgggctcccc gggcgcgtac tccactcccc gagccggtgc gggtgccagg
     4441 gcgtgccctt gggctccccg ggcgcgtact ccactcccct tcgcaagacc cttcctctat
     4501 ataaggaagt tcatttcatt tggagaggac tccggtattt ttacaacaat taccacaaca
     4561 aaacaaacaa caaacaacat tacaatttac tattctagtc gaatactgta tttttacaac
     4621 aattaccaac aacaacaaac aacaaacaac attacaatta ctatttacaa ttacaatggt
     4681 tgccacaatt gattccattg aaatgcctgc acttccaaca gctgtggaag cccacccaat
     4741 gaaaggtggt gatgactccc acagttactc ccaaaattct tgctaccaga aaggagtaat
     4801 tgatgcagcc aaggcagtga ttgttgaagc agtgaatgaa aaattggatc ttgaaaataa
     4861 tccaatcttt gatccaatca aacctttccg cattgcagat tttggatgtt caacaggacc
     4921 aaatactttc cacgctatgc aaaacatagt agaatcagtt gaaacaaaat acaaatcact
     4981 tcaaaaaacc cctgaattcc atgttttctt caatgatcat gttaacaatg atttcaatgt
     5041 tctctttaga tcccttccac caaacaggga attttttgct gctggtgttc ctggatcttt
     5101 ctacactaga gtttttccca aaaatagcat tcattttgct cattgttctt atgcacttca
     5161 ttggttatct aaagtgccca aggaaattca agataaaaat tctttggctt ataataaggg
     5221 aagaattcat tacactggta ctgaaaaaca tgttgttaag gcttattttg gtcaattcca
     5281 aagagatttt gaagggtttt taaaagcaag agctcaagaa attgttgttg gagggttgat
     5341 ggtgattcaa atacctgggc ttcctagtgg tgaagtcctt ttctcaagga ctggtgctgg
     5401 tttgcttcac ttccttttgg gaacttcctt gatggaattg gttaacaagg gaatcatcaa
     5461 tgaagaaagt gtagattcct tcaatttgcc tcaatatcat ccctcagttg aagatttgga
     5521 aatggtgata gaaatgaacg attgtttcac aattgaaagg gttggaactt tacctcatcc
     5581 catgaagaat ttaccatttg atgttcaaag gacttcttta caagtaagag caatcatgga
     5641 atgcattctc actgaacatt ttggggaaaa tattttggat ccattatttg aaatctacac
     5701 caaaaatttg caagagaatt tccatgtttt tgataaggaa attagaaagg atgcagattt
     5761 gtaccttgtc ttgaaacgca agggaaatta agcttgtcct gctttaatga gatatgcgag
     5821 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
     5881 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     5941 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     6001 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     6061 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     6121 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     6181 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     6241 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     6301 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     6361 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     6421 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     6481 tgcgacaaca tgtcgaggct cagcaggacc gctttacgaa ttcccatggg gagcggtgcg
     6541 ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cactccccga gccggtgcgg
     6601 gtgccagggc gtgcccttgg gctccccggg cgcgtactcc actcccccca tggtgcggtg
     6661 cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccactcccc gagccggtgc
     6721 gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccactcccct tcgcaagacc
     6781 cttcctctat ataaggaagt tcatttcatt tggagaggac tccggtattt ttacaacaat
     6841 taccacaaca aaacaaacaa caaacaacat tacaatttac tattctagtc gaatactgta
     6901 tttttacaac aattaccaac aacaacaaac aacaaacaac attacaatta ctatttacaa
     6961 ttacaatggg cagcattgat tcaacaaatg tagccatgtc caattctcca gttggagaat
     7021 ttaagccact tgaagctgag gaattccgaa aacaagccca tcgtatggta gatttcatag
     7081 ccgattatta caaaaatgtg gaaacatatc cggtccttag cgaagtcgaa cctggatatc
     7141 tccgaaaacg tatccccgaa accgctcctt acctccccga accacttgac gacatcatga
     7201 aagatattca gaaggatatt atcccaggaa tgacaaattg gatgagccct aatttttatg
     7261 cattttttcc tgccactgtt agttcagctg cctttttagg agaaatgttg tctactgccc
     7321 taaattcagt aggctttact tgggtttctt caccagccgc caccgaatta gaaatgattg
     7381 ttatggattg gttggctcag atccttaaac tccccaaatc tttcatgttt tcaggtaccg
     7441 gtggcggcgt catccaaaac accactagcg agtccattct ttgtacaatc attgccgccc
     7501 gggaaagggc cctggagaag ctcggtcccg atagtattgg aaaacttgtc tgttacggat
     7561 ccgatcaaac ccataccatg ttccccaaaa cttgcaaatt ggcgggaatt tatccgaata
     7621 atattaggtt aatacctacg accgtcgaaa cggatttcgg catctcacct caagttctac
     7681 gaaaaatggt cgaggatgac gtggcggccg gatatgtacc gctgttctta tgcgctaccc
     7741 tgggtaccac ctcgaccacg gctaccgatc ctgtggactc actttctgaa atcgctaacg
     7801 agtttggtat ttggatccac gtggatgctg cttatgcggg aagcgcctgt atatgtcccg
     7861 agtttagaca ttacttggat ggaatcgaac gagttgactc actgagtctg agtccacaca
     7921 aatggctact cgcttactta gattgcactt gcttgtgggt caagcaacca catttgttac
     7981 taagggcact cactacgaat cctgagtatt taaaaaataa acagagtgat ttagacaaag
     8041 ttgtggactt caaaaattgg caaatcgcaa cgggacgaaa atttcggtcg ctgaaacttt
     8101 ggctcatttt acgtagctat ggagttgtta atttacagag tcatattcgt tctgacgtcg
     8161 caatgggcaa aatgttcgaa gaatgggtta gatcagactc cagattcgaa attgtggtac
     8221 cgagaaactt ttctcttgtt tgttttagat taaaacctga cgtttcgagt ttacatgtag
     8281 aagaagtgaa taagaaactt ttggacatgc ttaactcgac gggacgagtt tatatgactc
     8341 atactattgt gggaggcata tacatgctaa gactggctgt tggctcgtca ctaactgaag
     8401 aacatcatgt acgccgtgtt tgggatttga ttcaaaaatt aaccgatgat ttgctcaaag
     8461 aagcttgagc ttgatccccc gtcgacagct agctatatca tcaatttatg tattacacat
     8521 aatatcgcac tcagtctttc atctacggca atgtaccagc tgatataatc agttattgaa
     8581 atatttctga atttaaactt gcatcaataa atttatgttt ttgcttggac tataatacct
     8641 gacttgttat tttatcaata aatatttaaa ctatatttct ttcaagatcg ctcagagaat
     8701 tcgcatgcgg agcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
     8761 actccccgag ccggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca
     8821 ctccccccat ggtgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact
     8881 ccactccccg agccggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc
     8941 cactcccctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggact
     9001 ccggtatttt tacaacaatt accacaacaa aacaaacaac aaacaacatt acaatttact
     9061 attctagtcg aatactgtat ttttacaaca attaccaaca acaacaaaca acaaacaaca
     9121 ttacaattac tatttacaat tacaatggag atggatatgg atatcattag aaaggcaatt
     9181 gctgccacta tctttgcatt ggttatggct tgggcgtgga gagtgttgga ttgggcatgg
     9241 tttactccta agaggatcga gaaacgtcta aggcagcaag gttttagagg aaatccttat
     9301 agattcttgg ttggagatgt taaggagagt ggaaaaatgc atcaagaagc cttgtctaac
     9361 cccatggagt tcgacaacga tattgttcct cgcctcatgc cacatattaa ccacaccata
     9421 aaaacttacg gtaggaattc atttacatgg atgggaagga ttccaaggat tcatgttatg
     9481 gaacctgaac ttattaagga agtattgacc cactccagca aataccaaaa gaactttgat
     9541 gttcacaatc cccttgttaa gttccttctc actggagttg gaagctttga aggggcaaaa
     9601 tggtccaaac acagaagaat catttcccct gccttcactc ttgagaaact aaagtcaatg
     9661 ttgccggctt ttgccatatg ctatcatgac atgttgacga aatgggagaa attagcagaa
     9721 aaagaaggat cccatgaagt tgatatcttc cccacatttg atgttttgac aagtgatgta
     9781 atttcaaagg ttgcatttgg tagcacatac gatgaaggag gcaaaatctt cagactattg
     9841 aaagaactca tggatctcac aattgattgc atgagagatg tctacatccc agggtggagc
     9901 tacttgccaa ccaagaggaa caagaggatg aaagaaatta acaaagaaat cacagatatg
     9961 ctaaggttca tcatcaacaa gagaatgaag gctttgaagg ctggagaacc aggtgaggat
    10021 gacttgttgg gagtcctgtt ggaatcaaac attcaagaaa ttcagaaaca aggaaacagg
    10081 aaggatggtg gaatgacaat caacgatgtg attgaggaat gcaaattgtt ctactttgct
    10141 ggtcaagaaa ccaccggagt tttgctcaca tggaccacca ttttattgag caagcatccc
    10201 gagtggcaag agcgagctag ggaagaagtt ctccaagcct ttggcaagaa caagcctgaa
    10261 tttgaacgct tgaatcatct taaatatgtt tctatgatct tatacgaggt tctaaggttg
    10321 tacccgccag ttattgatct gaccaagatt attcacgagg acacaaagtt agggccgtac
    10381 acaatccctg caggaacaca agtaatgttg ccgacagtaa tgcttcatag agagaagtcc
    10441 atttggggag aagatgcaat ggaattcaac ccaatgagat ttgctgatgg agttgccaat
    10501 gccaccaaga acaatgtcac atatttgcca ttcagttggg gacctagagt ttgcctcggc
    10561 caaaactttg cacttcttca agcaaaatta gggttggcaa tgattttaca acgcttcaaa
    10621 tttgatgtag ctccatccta tgttcatgct ccctttacca ttcttacagt tcaacctcag
    10681 tttggttctc atgttatcta caagaagctt gagagacaga atttttaagc ttgtcaagca
    10741 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
    10801 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
    10861 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
    10921 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
    10981 atgttactag atcgacgctt gtggaggatg cacatgtgac cgagggacac gaagtgatcc
    11041 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
    11101 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
    11161 atgtgcatgc caaccacagg gttcccctcg ggagtcagcc gtgcggctgc atgaaatcct
    11221 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
    11281 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
    11341 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
    11401 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
    11461 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
    11521 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
    11581 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
    11641 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
    11701 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
    11761 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
    11821 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
    11881 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
    11941 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
    12001 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
    12061 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
    12121 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
    12181 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
    12241 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
    12301 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
    12361 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
    12421 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
    12481 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
    12541 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
    12601 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gtcgtcgatt aggaagccgc
    12661 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
    12721 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
    12781 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
    12841 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
    12901 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
    12961 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
    13021 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
    13081 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
    13141 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
    13201 gcgagattac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
    13261 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
    13321 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
    13381 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
    13441 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
    13501 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
    13561 gggaaaaagg tcgaaaaagc ttctttcctg tggatagcac gtacattggg aacccaaagc
    13621 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
    13681 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
    13741 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
    13801 cagccgaaca gctgcaaaaa gcgcccacat caaggctccg agtgcgcgga acccctattt
    13861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    13921 tgcttcaata atattgaaaa aggaagagta tggctaaaat gagaatatca ccggaattga
    13981 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
    14041 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
    14101 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
    14161 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
    14221 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
    14281 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
    14341 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
    14401 tggccgatgt ggattgcgaa aactgggaag aggacactcc atttaaagat ccgcgcgagc
    14461 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
    14521 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
    14581 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg ctcagggagg
    14641 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
    14701 gggagaaaat aaaatattat attttactgg atgaattgtt ttagctgtca gaccaagttt
    14761 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    14821 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    14881 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    14941 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    15001 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    15061 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    15121 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    15181 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    15241 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    15301 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    15361 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    15421 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    15481 caggggggcg gagcctatgg aaaaacg
//
