LOCUS       sequence-349582-       15507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 2 plasmid containing multiple transcriptional units for 
            transient expression of CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH 
            driven by 4x opaatB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15507)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 15507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD2KN0899
FEATURES             Location/Qualifiers
     source          1..15507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    161..185
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    282..315
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    341..374
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    404..437
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    463..496
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        513..559
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    649..704
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     polyA_signal    1799..1973
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    2005..2038
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2064..2097
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2127..2160
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    2186..2219
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        2236..2282
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    2372..2427
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      4025..4277
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4312..4345
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    4371..4404
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    4434..4467
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    4493..4526
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        4543..4589
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    4679..4734
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      5859..6566
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    6602..6635
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6661..6694
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6724..6757
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    6783..6816
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        6833..6879
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    6969..7024
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      8548..8748
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    8781..8814
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    8840..8873
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    8903..8936
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    8962..8995
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        9012..9058
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    9148..9203
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      10772..11024
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    11088..11112
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11421..12050
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12479..13552
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13618..13812
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        13874..13978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             13979..14773
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      join(14944..15507,1..25)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15433..15452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctgct
       61 cggatctgtt ggaccggaca gtagtcatgg ttgatgggct gcctgtatcg agtggtgatt
      121 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
      181 taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta atgtactggg
      241 gttgaacact ctgtgccgaa ttcggatccg gagcggtgcg ggtgccaggg cgtgcccttg
      301 ggctccccgg gcgcgtactc cactccccga gccggtgcgg gtgccagggc gtgcccttgg
      361 gctccccggg cgcgtactcc actcccccca tggtgcggtg cgggtgccag ggcgtgccct
      421 tgggctcccc gggcgcgtac tccactcccc gagccggtgc gggtgccagg gcgtgccctt
      481 gggctccccg ggcgcgtact ccactcccct tcgcaagacc cttcctctat ataaggaagt
      541 tcatttcatt tggagaggac tccggtattt ttacaacaat taccacaaca aaacaaacaa
      601 caaacaacat tacaatttac tattctagtc gaatactgta tttttacaac aattaccaac
      661 aacaacaaac aacaaacaac attacaatta ctatttacaa ttacaatggc aaacttttct
      721 gaatctaaat ccatgatggc agttttcttc atgtttttcc tgcttctttt gtcatcttct
      781 agttcttcat cttcaagttc accaattttg aaaaagattt ttattgaaag cccttcctat
      841 gctccgaatg ccttcacctt cgattcaact gataaagggt tctacacttc cgtccaagat
      901 ggccgagtta tcaaatatga agggccaaat tcaggcttca ctgacttcgc ctacgcatct
      961 cccttctgga acaaagcttt ttgtgagaac agcaccgatc cagagaaaag accattgtgt
     1021 gggaggacat atgatatttc ctatgactat aagaacagcc aaatgtacat tgttgatggc
     1081 cattaccatc tttgtgtggt tggaaaagaa ggtgggtatg ccacacaact agccacaagt
     1141 gtgcaaggag tgccattcaa atggctctat gcagtaactg ttgatcagag aacagggatt
     1201 gtttatttca ctgatgttag ctccatacat gatgacagtc ccgaaggtgt ggaagaaatc
     1261 atgaatacaa gtgatagaac agggagatta atgaagtatg atccttcaac aaaagaaacc
     1321 accttattat tgaaagagct acatgttccc ggcggtgcag aaatcagcgc agatggttcc
     1381 tttgttgtag tagcagaatt tttaagcaat cggatagtga agtattggct agaagggcca
     1441 aagaaaggca gtgcagagtt cttagttaca atcccaaatc caggaaatat aaagaggaat
     1501 tctgatggcc atttttgggt gtcatctagt gaagaattag atggaggtca acatggaaga
     1561 gttgtttcaa gaggaattaa gtttgatgga tttgggaata ttcttcaagt tataccactt
     1621 ccaccaccat atgaaggtga acattttgaa cagattcaag agcacgatgg tttgttatac
     1681 attggaagtc tcttccatag ctctgtgggt atattagtgt atgatgatca tgataacaag
     1741 ggaaattctt atgtttctag ctgagcttct ctagctagag tcgatcgaca agctcgagtt
     1801 tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc
     1861 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     1921 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atcgctgcaa
     1981 gaattcaagc ttggagcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta
     2041 ctccactccc cgagccggtg cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac
     2101 tccactcccc ccatggtgcg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg
     2161 tactccactc cccgagccgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt
     2221 actccactcc ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
     2281 gactccggta tttttacaac aattaccaca acaaaacaaa caacaaacaa cattacaatt
     2341 tactattcta gtcgaatact gtatttttac aacaattacc aacaacaaca aacaacaaac
     2401 aacattacaa ttactattta caattacaat ggagatggat atggatatca ttagaaaggc
     2461 aattgctgcc actatctttg cattggttat ggcttgggcg tggagagtgt tggattgggc
     2521 atggtttact cctaagagga tcgagaaacg tctaaggcag caaggtttta gaggaaatcc
     2581 ttatagattc ttggttggag atgttaagga gagtggaaaa atgcatcaag aagccttgtc
     2641 taaccccatg gagttcgaca acgatattgt tcctcgcctc atgccacata ttaaccacac
     2701 cataaaaact tacggtagga attcatttac atggatggga aggattccaa ggattcatgt
     2761 tatggaacct gaacttatta aggaagtatt gacccactcc agcaaatacc aaaagaactt
     2821 tgatgttcac aatccccttg ttaagttcct tctcactgga gttggaagct ttgaaggggc
     2881 aaaatggtcc aaacacagaa gaatcatttc ccctgccttc actcttgaga aactaaagtc
     2941 aatgttgccg gcttttgcca tatgctatca tgacatgttg acgaaatggg agaaattagc
     3001 agaaaaagaa ggatcccatg aagttgatat cttccccaca tttgatgttt tgacaagtga
     3061 tgtaatttca aaggttgcat ttggtagcac atacgatgaa ggaggcaaaa tcttcagact
     3121 attgaaagaa ctcatggatc tcacaattga ttgcatgaga gatgtctaca tcccagggtg
     3181 gagctacttg ccaaccaaga ggaacaagag gatgaaagaa attaacaaag aaatcacaga
     3241 tatgctaagg ttcatcatca acaagagaat gaaggctttg aaggctggag aaccaggtga
     3301 ggatgacttg ttgggagtcc tgttggaatc aaacattcaa gaaattcaga aacaaggaaa
     3361 caggaaggat ggtggaatga caatcaacga tgtgattgag gaatgcaaat tgttctactt
     3421 tgctggtcaa gaaaccaccg gagttttgct cacatggacc accattttat tgagcaagca
     3481 tcccgagtgg caagagcgag ctagggaaga agttctccaa gcctttggca agaacaagcc
     3541 tgaatttgaa cgcttgaatc atcttaaata tgtttctatg atcttatacg aggttctaag
     3601 gttgtacccg ccagttattg atctgaccaa gattattcac gaggacacaa agttagggcc
     3661 gtacacaatc cctgcaggaa cacaagtaat gttgccgaca gtaatgcttc atagagagaa
     3721 gtccatttgg ggagaagatg caatggaatt caacccaatg agatttgctg atggagttgc
     3781 caatgccacc aagaacaatg tcacatattt gccattcagt tggggaccta gagtttgcct
     3841 cggccaaaac tttgcacttc ttcaagcaaa attagggttg gcaatgattt tacaacgctt
     3901 caaatttgat gtagctccat cctatgttca tgctcccttt accattctta cagttcaacc
     3961 tcagtttggt tctcatgtta tctacaagaa gcttgagaga cagaattttt aagcttgtca
     4021 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     4081 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     4141 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     4201 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     4261 atctatgtta ctagatcgac gctactagaa ttcgagctcg gagcggtgcg ggtgccaggg
     4321 cgtgcccttg ggctccccgg gcgcgtactc cactccccga gccggtgcgg gtgccagggc
     4381 gtgcccttgg gctccccggg cgcgtactcc actcccccca tggtgcggtg cgggtgccag
     4441 ggcgtgccct tgggctcccc gggcgcgtac tccactcccc gagccggtgc gggtgccagg
     4501 gcgtgccctt gggctccccg ggcgcgtact ccactcccct tcgcaagacc cttcctctat
     4561 ataaggaagt tcatttcatt tggagaggac tccggtattt ttacaacaat taccacaaca
     4621 aaacaaacaa caaacaacat tacaatttac tattctagtc gaatactgta tttttacaac
     4681 aattaccaac aacaacaaac aacaaacaac attacaatta ctatttacaa ttacaatggt
     4741 tgccacaatt gattccattg aaatgcctgc acttccaaca gctgtggaag cccacccaat
     4801 gaaaggtggt gatgactccc acagttactc ccaaaattct tgctaccaga aaggagtaat
     4861 tgatgcagcc aaggcagtga ttgttgaagc agtgaatgaa aaattggatc ttgaaaataa
     4921 tccaatcttt gatccaatca aacctttccg cattgcagat tttggatgtt caacaggacc
     4981 aaatactttc cacgctatgc aaaacatagt agaatcagtt gaaacaaaat acaaatcact
     5041 tcaaaaaacc cctgaattcc atgttttctt caatgatcat gttaacaatg atttcaatgt
     5101 tctctttaga tcccttccac caaacaggga attttttgct gctggtgttc ctggatcttt
     5161 ctacactaga gtttttccca aaaatagcat tcattttgct cattgttctt atgcacttca
     5221 ttggttatct aaagtgccca aggaaattca agataaaaat tctttggctt ataataaggg
     5281 aagaattcat tacactggta ctgaaaaaca tgttgttaag gcttattttg gtcaattcca
     5341 aagagatttt gaagggtttt taaaagcaag agctcaagaa attgttgttg gagggttgat
     5401 ggtgattcaa atacctgggc ttcctagtgg tgaagtcctt ttctcaagga ctggtgctgg
     5461 tttgcttcac ttccttttgg gaacttcctt gatggaattg gttaacaagg gaatcatcaa
     5521 tgaagaaagt gtagattcct tcaatttgcc tcaatatcat ccctcagttg aagatttgga
     5581 aatggtgata gaaatgaacg attgtttcac aattgaaagg gttggaactt tacctcatcc
     5641 catgaagaat ttaccatttg atgttcaaag gacttcttta caagtaagag caatcatgga
     5701 atgcattctc actgaacatt ttggggaaaa tattttggat ccattatttg aaatctacac
     5761 caaaaatttg caagagaatt tccatgtttt tgataaggaa attagaaagg atgcagattt
     5821 gtaccttgtc ttgaaacgca agggaaatta agcttgtcct gctttaatga gatatgcgag
     5881 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
     5941 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     6001 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     6061 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     6121 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     6181 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     6241 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     6301 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     6361 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     6421 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     6481 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     6541 tgcgacaaca tgtcgaggct cagcaggacc gctttacgaa ttcccatggg gagcggtgcg
     6601 ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cactccccga gccggtgcgg
     6661 gtgccagggc gtgcccttgg gctccccggg cgcgtactcc actcccccca tggtgcggtg
     6721 cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccactcccc gagccggtgc
     6781 gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccactcccct tcgcaagacc
     6841 cttcctctat ataaggaagt tcatttcatt tggagaggac tccggtattt ttacaacaat
     6901 taccacaaca aaacaaacaa caaacaacat tacaatttac tattctagtc gaatactgta
     6961 tttttacaac aattaccaac aacaacaaac aacaaacaac attacaatta ctatttacaa
     7021 ttacaatggg cagcattgat tcaacaaatg tagccatgtc caattctcca gttggagaat
     7081 ttaagccact tgaagctgag gaattccgaa aacaagccca tcgtatggta gatttcatag
     7141 ccgattatta caaaaatgtg gaaacatatc cggtccttag cgaagtcgaa cctggatatc
     7201 tccgaaaacg tatccccgaa accgctcctt acctccccga accacttgac gacatcatga
     7261 aagatattca gaaggatatt atcccaggaa tgacaaattg gatgagccct aatttttatg
     7321 cattttttcc tgccactgtt agttcagctg cctttttagg agaaatgttg tctactgccc
     7381 taaattcagt aggctttact tgggtttctt caccagccgc caccgaatta gaaatgattg
     7441 ttatggattg gttggctcag atccttaaac tccccaaatc tttcatgttt tcaggtaccg
     7501 gtggcggcgt catccaaaac accactagcg agtccattct ttgtacaatc attgccgccc
     7561 gggaaagggc cctggagaag ctcggtcccg atagtattgg aaaacttgtc tgttacggat
     7621 ccgatcaaac ccataccatg ttccccaaaa cttgcaaatt ggcgggaatt tatccgaata
     7681 atattaggtt aatacctacg accgtcgaaa cggatttcgg catctcacct caagttctac
     7741 gaaaaatggt cgaggatgac gtggcggccg gatatgtacc gctgttctta tgcgctaccc
     7801 tgggtaccac ctcgaccacg gctaccgatc ctgtggactc actttctgaa atcgctaacg
     7861 agtttggtat ttggatccac gtggatgctg cttatgcggg aagcgcctgt atatgtcccg
     7921 agtttagaca ttacttggat ggaatcgaac gagttgactc actgagtctg agtccacaca
     7981 aatggctact cgcttactta gattgcactt gcttgtgggt caagcaacca catttgttac
     8041 taagggcact cactacgaat cctgagtatt taaaaaataa acagagtgat ttagacaaag
     8101 ttgtggactt caaaaattgg caaatcgcaa cgggacgaaa atttcggtcg ctgaaacttt
     8161 ggctcatttt acgtagctat ggagttgtta atttacagag tcatattcgt tctgacgtcg
     8221 caatgggcaa aatgttcgaa gaatgggtta gatcagactc cagattcgaa attgtggtac
     8281 cgagaaactt ttctcttgtt tgttttagat taaaacctga cgtttcgagt ttacatgtag
     8341 aagaagtgaa taagaaactt ttggacatgc ttaactcgac gggacgagtt tatatgactc
     8401 atactattgt gggaggcata tacatgctaa gactggctgt tggctcgtca ctaactgaag
     8461 aacatcatgt acgccgtgtt tgggatttga ttcaaaaatt aaccgatgat ttgctcaaag
     8521 aagcttgagc ttgatccccc gtcgacagct agctatatca tcaatttatg tattacacat
     8581 aatatcgcac tcagtctttc atctacggca atgtaccagc tgatataatc agttattgaa
     8641 atatttctga atttaaactt gcatcaataa atttatgttt ttgcttggac tataatacct
     8701 gacttgttat tttatcaata aatatttaaa ctatatttct ttcaagatcg ctcagagaat
     8761 tcgcatgcgg agcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
     8821 actccccgag ccggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca
     8881 ctccccccat ggtgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact
     8941 ccactccccg agccggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc
     9001 cactcccctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggact
     9061 ccggtatttt tacaacaatt accacaacaa aacaaacaac aaacaacatt acaatttact
     9121 attctagtcg aatactgtat ttttacaaca attaccaaca acaacaaaca acaaacaaca
     9181 ttacaattac tatttacaat tacaatggaa ttgaacttca aatcaattat tttcttggtt
     9241 tttgttagtc taacccttta ctgggtctat agaatcttgg attgggtatg gtttaagccc
     9301 aagaagctgg aaaaatgctt aagagaacaa gggtttaagg gaaatcctta cagattgttc
     9361 ttgggagatc agtatgatag tggaaaattg atcagacaag ccttgactaa acctattggt
     9421 gttgaagaag atgttaagaa acgaattgta cctcatatac tcaaaactgt tgggactcat
     9481 ggcaagaagt cttttatgtg ggttggaaga ataccaaggg tgaatatcac agatccagag
     9541 ctgattaaag aggtcttaac aaaatactat aagttccaga agaatcatca tgatcttgac
     9601 ccaattacca aattgctttt gactggaatt ggaagcttag aaggtgaccc ttgggctaaa
     9661 cgccgaaaaa tcatcaatgc tgcttttcac tttgaaaagt taaagcttat gcttcctgca
     9721 ttctatttga gttgccgtga tatggtgaca aaatgggata ataaggtccc tgaaggagga
     9781 tcagcagagg tggatgtgtg gcatgatatt gaaaccttaa ctggagatgt aatttcaaga
     9841 acattatttg gaagtaactt cgaggaaggg agaagaatat ttgaactcat gaaagaactt
     9901 actgctctta ctattgatgt cattcgctcg gtttatatcc ctggacagag gtttcttcca
     9961 actaagagga acaataggat gagagcaatt gacaaagaag ttagagtaag aataacagaa
    10021 attatcaata agaaaatgaa ggtaatgaaa tcaggagaag cagcaagtgc tgctgatgat
    10081 tttttgggaa tattgttgga atgcaatctg aatgaaataa aggagcaagg aaataacaaa
    10141 agtgctggaa tgactattgg agagattatt ggtgagtgca aactcttcta ttttgctggc
    10201 caggacacta cctcgacttt actcgtgtgg acaatggtcc tcttgtctag attccccgaa
    10261 tggcaaactc gtgccagaga agaagttttt caagtctttg gtaataaaac cccggactat
    10321 gatggtatca gccatcttaa agttataacg atgatcttgt acgaggtttt gaggttatat
    10381 actccagtgg ctgaactgac aaaagtggct catgaagcta cacaactggg aaaatacttc
    10441 attccagcgg gcgtgcagct aatgatgcca caaatcctac ttcatcatga ccctgaaata
    10501 tggggtgaag atgtaatgga gttcaaacca gaaagatttg cagagggagt tctcaaggca
    10561 actaagagcc aaggctcttt ttttccattt agcttgggac caagaatgtg cattggccaa
    10621 aactttgctc tattggaagc caaaatggca atgtctctaa tcttgcgtag attttctttc
    10681 gagctttctc cctcctatgt tcatgctcct ttcactctca tcacaatgca accccaatat
    10741 ggtgctcatt tgattctgca caaactctaa gcttggactc ccatgttggc aaaggcaacc
    10801 aaacaaacaa tgaatgatcc gctcctgcat atggggcggt ttgagtattt caactgccat
    10861 ttgggctgaa ttgtagacat gctcctgtca gaaattccgt gatcttactc aatattcagt
    10921 aatctcggcc aatatcctaa atgtgcgtgg ctttatctgt ctttgtattg tttcatcaat
    10981 tcatgtaacg tttgcttttc ttatgaattt tcaaataaat tatccgcttg tggaggatgc
    11041 acatgtgacc gagggacacg aagtgatccg tttaaactat cagtgtttga caggatatat
    11101 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
    11161 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
    11221 gagtcagccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
    11281 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
    11341 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
    11401 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
    11461 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
    11521 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
    11581 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
    11641 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
    11701 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
    11761 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
    11821 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
    11881 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
    11941 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
    12001 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
    12061 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
    12121 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
    12181 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
    12241 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
    12301 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
    12361 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
    12421 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
    12481 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
    12541 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
    12601 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
    12661 agccggtgcg tcgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
    12721 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
    12781 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
    12841 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
    12901 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
    12961 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
    13021 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
    13081 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
    13141 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
    13201 cgagatcgag ctagctgatt ggatgtaccg cgagattaca gaaggcaaga acccggacgt
    13261 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
    13321 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
    13381 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
    13441 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
    13501 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
    13561 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaagct tctttcctgt
    13621 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
    13681 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
    13741 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
    13801 ggcctgtgca taactgtctg gccagcgcac agccgaacag ctgcaaaaag cgcccacatc
    13861 aaggctccga gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    13921 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    13981 ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa
    14041 agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct
    14101 atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa
    14161 ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg
    14221 gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga
    14281 gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct
    14341 ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc
    14401 cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga
    14461 ggacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga
    14521 agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg
    14581 caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat
    14641 tgccttctgc gtccggtcgc tcagggagga tatcggggaa gaacagtatg tcgagctatt
    14701 ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga
    14761 tgaattgttt tagctgtcag accaagttta ctcatatata ctttagattg atttaaaact
    14821 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    14881 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    14941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    15001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    15061 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    15121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    15181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    15241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    15301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    15361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    15421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    15481 acttgagcgt cgatttttgt gatgctc
//
