LOCUS       sequence-349583-        5524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control vector for Flp-dependent DREADD constructs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5524)
  TITLE     Flp-dependent mCherry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-fDIO-mCherry-WPREpA
FEATURES             Location/Qualifiers
     source          1..5524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        121..568
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    606..639
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(732..1442)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1010..1029)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1279..1297
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1439..1448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1455..1488)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    1597..2185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1650..1670)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2068..2079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2217..2693
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2416..2435)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2733..2873
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2733..2862
                     /label=AAV2 ITR
     rep_origin      2948..3403
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3035..3054)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3245..3266
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3420..3439)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3539..3561
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3599..3617)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3685..3789
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3790..3858,3859..4650)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4008..4027)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4821..5409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5310..5329
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(5471..5524,1..76)
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
       61 atcactaggg gttcctgcgg ccgcacgcgt gtgtctagac tgcagagggc cctgcgtatg
      121 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      181 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      241 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      301 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      361 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      421 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      481 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      541 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggtac cggatcctct agagtcgact
      601 ccggagaagt tcctattctc tagaaagtat aggaacttcg cagaatggta gctggattgt
      661 agctgctatt agcaatatga aacctcttag aagttcctat tcttcaaaag gtataggaac
      721 ttcggcgcgc cttacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg
      781 cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg
      841 ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc
      901 tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg
      961 gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc
     1021 attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg
     1081 ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
     1141 acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg
     1201 gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg
     1261 aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
     1321 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc
     1381 accttgaagc gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc
     1441 atggtggcgc tagcgaagtt cctatacttt ctagagaata ggaacttctt gccttaaccc
     1501 agaaattatc actgttattc tttagaatgg tgcaaagaga agttcctata ccttttgaag
     1561 aataggaact tcgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     1621 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     1681 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     1741 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     1801 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     1861 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     1921 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     1981 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg
     2041 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     2101 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     2161 atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt
     2221 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
     2281 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
     2341 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
     2401 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
     2461 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
     2521 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
     2581 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
     2641 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
     2701 tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
     2761 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     2821 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     2881 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     2941 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3001 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
     3061 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3121 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     3181 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3241 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
     3301 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
     3361 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     3421 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     3481 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     3541 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     3601 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     3661 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3721 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3781 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     3841 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     3901 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     3961 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4021 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4081 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4141 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4201 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4261 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4321 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4381 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4441 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4501 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4561 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4621 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4681 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4741 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4801 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4861 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4921 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     4981 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5041 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5101 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5161 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5221 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5281 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5341 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5401 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5461 gctcacatgt cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg
     5521 ggcg
//
