LOCUS       sequence-349589-        6897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon 
            optimized for expression in Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Bejenari M, Nielsen L, Spedtsberg EML, Nielsen MR, Pedersen TB, 
            Sorensen JL
  TITLE     Yeast recombinational cloning for heterologous biosynthesis of 
            polyketides: a molecular microbiology laboratory module for 
            undergraduate students.
  JOURNAL   J Microbiol Biol Educ. 2023 Nov 27;24(3):e00242-22. doi: 
            10.1128/jmbe.00242-22. eCollection 2023 Dec.
  PUBMED    38108002
REFERENCE   2  (bases 1 to 6897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/jmbe.00242-22"; journalName: "J Microbiol Biol Educ"; date:
            "2023-11-27- 27"; volume: "24"; issue: "3"; pages: "e00242-22"
COMMENT     Sequence Label: pUC57-fsr1
FEATURES             Location/Qualifiers
     source          1..6897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgacagaca acttaaaatt atacttattc ggtgaccaaa ccttcgacat caagccacac
       61 ttacaaaaat tattccaaca aagagaccat ttgtttttgc aagatttctt gacaaaggct
      121 tacaacgcaa tcagagttga aatgtacaga ttgcctttta aagttagaaa cgatttgcca
      181 agattcactt gtcaagaaga tttgttaaga tgggatgaat ctggtaaaag atgcatcgct
      241 ttggatatgg caatgactac attgtaccat ttgggtactt ttatttcaca agctggtatc
      301 tcttcatacg atgcacaatc ttcaagagtt gttggtttgt gtacaggtgc ttttgctgca
      361 gctgcaatct cttgttcttc ttttactgct gatttgattc caatggcagt ttattcagtt
      421 attgctgctt ttagaacagg catgttggtt actgatgttg caaaaagagt tgatcaatct
      481 caagatttgg atcaatcatg ggctttgtta gttccaggtt ctaaatcagc tgcagctgtt
      541 gaaaagttct gtgaagaatc taatttgcca ttgacttcta gaccatatat ttcagcatac
      601 gctccaaatg gtattacagt ttctggtcca ccaaaaactt tagctcaatt gatctcttca
      661 ccatctttta aaggtttgac atctaagtca atcccaatct tcggtgctta ccatgcacca
      721 catttgtact ctcaaatgga tgctaagaaa attgtttctg gtttatcata caatgcagct
      781 actgctttgt ctgaacaaat tccattgtta tcttcaacag gtgttaagtt ggaagaaaga
      841 tcattcgcta ctttgttaga agatgcagtt gctcaagcat tagttcatcc attgcattgg
      901 tcttcaattt taggtgacat tcaagcttgt ttgcaagatg ttaatccaga acaattttct
      961 gttgttccaa ttggttcaac tgctgatcat ttgatctata cagcattgaa gcaaactcca
     1021 ttaagatctt tggttccaaa tacacatggt caaggtagac aatcagttcc agatgttggt
     1081 gttgaatcta cttctaagaa accaaagttg gctatcgttg caatgtctgg tagatttcca
     1141 ggtgctaaag ataatgaagc atactgggat ttgttgttcc aaggtttaga tgttcataag
     1201 ccagttccat ctttgagatg ggatgcacaa acacatgttg atccaacagg taaaactaaa
     1261 aatacatcag ctactccatt tggttgttgg ttggatgatc cagctgaatt cgatgcaaga
     1321 tttttcaaca tctctccaag agaagctcca caaattgatc cagctcaaag aattgcatta
     1381 atgactgctt atgaagcaat tgaacaagct ggtattgttc cagatgcaac accatctact
     1441 agaccagata gagttggtgt tttctatggt gtcacatcta acgattggat ggaaactaac
     1501 tcagcacaag gtattgatac atactttatt ccaggtggta atagagcttt tattccaggt
     1561 agaattaatt atttctttaa attttctggt ccatcatacg ctgttgatac tgcatgttct
     1621 tcatctttag ctggtattca tttggcatgt aattctttat ggagaggtga cattgataca
     1681 gctattgcag gtggtacaaa cgttttgact aacccagatt tcacttctgg tttggataga
     1741 ggtcatttct tgtcaagaac tggtaactgt aagactttcg atgatggtgc tgatggttat
     1801 tgtagaggtg aaggtgttgc aacattgatt attaagagat tggatgatgc tatcgcagaa
     1861 aacgatccaa tcttgggtgt tattttgggt gcttacacaa atcattctgc agaatctgaa
     1921 tcaattacta gaccacatgt tggtgctcaa agagcaattt tctctaagat cttgtcagaa
     1981 gcagctgttg atccatatac tgtttcttac gttgaaatgc atggtacagg tactcaagct
     2041 ggtgacgcaa cagaaatggc ttcagtttta gatacttttg caccaccatt ggttaatggt
     2101 aaaaaggcta gatctgatga acaagcatta tttttgggtt cagctaaggc aaacattggt
     2161 catggtgaag cagcttctgg tgcttcatct gttattaaag ttttgaacat gatgcaaaag
     2221 aatatgatcg ttccacattg tggtattaaa acaaagatta atcataagtt tccaactgat
     2281 ttgttgcaaa gaaacgttca tatcgctttg aagccaactc catgggatag aattaatggt
     2341 ccaagaagag ttttcgttaa caacttctct gcagctggtg gtaattcagc tttgttattg
     2401 gaagatgcac caccaaaagt tgaaagaaga gcaacagttg attctagagt tcaatttcca
     2461 attgctgtta ctgcaaaatc tggttcagct ttgcagggta acatgagatc aatgttgaag
     2521 ttcttgaaag aaaatccaga agtttctttg ggtgaattgt catacactac aactgctaga
     2581 agaatccatc atcaacatag agttttgttg aatggtccaa caactgaaga tatctgtaag
     2641 aaaattgaaa cagctttgca aaacaacact ggtgttaaca gaccaaagtc tgcaccatct
     2701 gttattttta cttttactgg tcaaggtgct caataccctg gtatgggtaa acaattgttc
     2761 gaagaatcat ctttcgttag atctgaattg atccaattgg atcaaatcgc tcaatctttg
     2821 ggttttccat caatgttacc agttattcaa tctgaagaac aagatatcgg tatttttgct
     2881 ccaacagcag ttcaattggc atcagtttgt ttgcaaatca ctttgtccaa gttgtgggct
     2941 tcatggggta tctatccagc agctgttgtt ggtcattctt tgggtgaata cgcagcttta
     3001 aatgttgcag gtgttttgtc agatacagat actttatttt tggttggtaa aagagctcaa
     3061 ttgttggaac aaaagtgtac aagatctact cattcaatgt tggttgttaa aggttctgaa
     3121 gatgaaatcg ctgaagtttt gaagggtaac gaatacgaaa ctgcatgtat caactcacca
     3181 atcgaaacag ttttagctgg tactaacgaa caagttgcag atttgaagga attgttgaca
     3241 gcagcttcta tgaagtcaac tttgttgaag gttccatacg cattccattc atctcaattg
     3301 gatccagttt tgtcagaatt tcaagaaatt gctacaggtg ttactttttc taaaccatca
     3361 gttccagttt taagaccatt ggatggtaca gttgttgatc attgtgattc ttttggtcca
     3421 gaatatttgg ctaaccattc tagacaatca gttaacatgt taggtgcttt gtctacagca
     3481 tacagagatc atgttatcac tgataagtca atgatcttgg aattgggtcc acatccagct
     3541 attactggta tggttaaggc agttttgggt caacaagtta tttgtatcgc ttctttgcaa
     3601 agagcaagac aaccatggga tgttttgtgt gcagctttga aggttttgta tgatgctggt
     3661 gcaaatattt catgggcaga ataccaaaga gattttggtg gttttcattc tgttgttcca
     3721 ttaccagctt actcatggga tttgaaggat tactggatcc aatacgttaa cgattggtct
     3781 ttgagaaaag gtgacgctcc aatcgttatt aataacgcac caagattgga atctacaact
     3841 atccattcag ttgttgaaga atctggtgac tctaagaaaa ctcatatgat cgttgaagct
     3901 gatatctcta gaaaggatat gtcaccattg gttcaaggtc atgaagttga tggtattcca
     3961 ttatgtacac catctgttta cgctgatatg gcattaactt tgggtagata tttgttggaa
     4021 agataccaac cacaaaagaa agaaaatttg atcgatgttt ctgatatgac aatctcaaag
     4081 gctttgatct tgagaggtga cggttctaaa caaccattac aagctcattt ggatgctgat
     4141 tgggcatctc aatcagcaac tattaagttt atgactttcg ataataaggg taatttgcaa
     4201 aagcattctg aatgtgttgt tagattcaga gatcattcat tgcaaaagac tttgcaagat
     4261 aaggctgttt ctgttaagca aaagatgcaa gcattgagaa atggtattgc tacaggtgaa
     4321 actgcaagat acaacagagc tatggttttt agagcaatta gaccattagc tagattccat
     4381 gatgattaca gagcaatcga tgaaatcatc ttgaattctc caacatacga agcttcatct
     4441 caattgtctt tcggttcagt taagagagat ggtaacttcc atacacatcc agcaatcatc
     4501 gattcattga ctcaaggttg tggtttcgct atgaactgta acgatgatac tgatttggat
     4561 gttgaagttt ttatgaacca tggttggggt tctttacaaa tgttcgaacc attgcaattc
     4621 gataagacat acacaactta cactcaaatg aggccaggtg aagataaatt gtggcatggt
     4681 gacgttgtta tttttgatgg tgacagagtt gttgctttct ttggtcaaat tgcaattcaa
     4741 ggtgttccaa gaagagtttt gaaagttatt ttgtctttgg aatcaggtaa aaagactcaa
     4801 ccacaaagac cagctcaaaa taagacacca actatctcta tctcagctcc agcaaatggt
     4861 tcatctggta tccaaaagtt gccattgcca gttgaaaagg ttcaaccatc tagaatctca
     4921 acagcattga ctatcattgc tgaagaatct ggtattgatg ttgctgattt gacagatggt
     4981 actaacttct ctgatgttgg tatcgattca ttgttgggtt tgactatctc agcaagattc
     5041 agagaagaat tggatatcga tttggatttc aacgctttgt ttttcgatca tccaacagtt
     5101 caagatttga agattttctt gatgggtcca gatgatgatg gttctgctac ttcatcttca
     5161 aatgcatctg attcaggttt agaatctcca ccatcaggtt tgattacagg tgcagttact
     5221 ccagatgctc atgaagattt cgcaaaccca ttcgaagttg atttcttgag agctttggat
     5281 atcatctctg aagaatcagg tgttgcaaga gaagaattgg atgatgatac aaacttcgct
     5341 gattgtggtg ttgattcttt gttgtcattg gttattactt ctagattcca agatgctttc
     5401 ggtttagatg ttggtcatga aacattattt ttggattgtc aaactgttgg tgacttgaaa
     5461 gaaatgttga tgagagaaat gggtggtact ccaccagttc catctgttcc agttaataag
     5521 ccagcagctt taccagttcc agaagctgtt caagcatctc catcaaagac agttgttgtt
     5581 aacaacgctc attctggtac ttcagatttg gatgctagac aaaaggcagt tgatgaattg
     5641 gttcaaaagt acacagctgg tttttctgca ccaacttctt caccatcagc tgttttgcca
     5701 gcaaacaacg aaaaggttgt tttgattaca ggtgcaactg gtggtttagg ttctcatttg
     5761 gtttactcaa tcgctcaatt ggaagatgtt aagacagtta tttgtttgaa tagagataat
     5821 agagaaggtc cagaaactag acaatacaag gctatgagag aaaagggtat cagattccca
     5881 gaaaatttga agcataagtt gagaatcttc caaacagatt cttcaaagcc acatttgggt
     5941 ttggataagg gtgactatga taatttggtt ggttctgtta ctcatttgat tcataatgct
     6001 tggccaatgt cagcaaaaag accattgtct ggtttcgaat cacaattcca agtttttaga
     6061 aatttggttg attttggtaa tgaagcagct tctttaagac cagaatcttt taaattttca
     6121 ttccaaatgg tttcttcaat tggtgttgtt ggtcaatacg gtttagcagc tggtcaaaca
     6181 gaaaagactg ttgttccaga aaaatctgct acagtcgact ctttgttgtc aaacggttat
     6241 gctgaagcaa aatggggttg tgaaagaatg ttggatgaaa ctttgcataa gtacccaaac
     6301 agattcagag ccatggttgt tagattgggt caaattgcag gttctaaaac atcaggttat
     6361 tggaatccaa tggaacattt cggtttcttg attaaatctt cacaaacttt gaatgctttg
     6421 ccagatgttg atggtgcatt gaattggaca ccagttaacg atatcgctga tactttgtct
     6481 gatttggttt tgtcagatag aacaccacat ccattttacc atattgataa tccagttggt
     6541 caacaatgga gagatgttaa cgctatcttg tctgatactt tgagaatccc aaatttggtt
     6601 ccttttaaag actggttaaa gttggttaga gaagctccac aacaagataa tccagcagct
     6661 ttgttggttg atttcttgga atcaacatat ttgagaatgg cttgtggtgg tttagttttg
     6721 gatgttaaaa atactttgga acattctaag tcattgagag ctgttggtcc agtttctgaa
     6781 gttgttacta gaaagtacat ccatatctgg aaagaaattg gtttcttgaa aacaactgca
     6841 gaagataagg caggttttga agccgaaaga ttgagattgt ggggtccaag agtttga
//
