LOCUS       sequence-349597-        7124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An improved vector for BacMam system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Guo W, Wang M, Chen L
  TITLE     A co-expression vector for baculovirus-mediated protein expression 
            in mammalian cells.
  JOURNAL   Biochem Biophys Res Commun. 2022 Feb 26;594:69-73. doi: 
            10.1016/j.bbrc.2022.01.056. Epub 2022 Jan 19.
  PUBMED    35074588
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "2022-02-26"; volume: "594:69-73. doi"; 
            pages: " 10.1016/j.bbrc.2022.01.056. Epub 2022 Jan 19"
COMMENT     Sequence Label: pBMCL1
FEATURES             Location/Qualifiers
     source          1..7124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  183..348
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    546..594
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     619..638
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(1049..1081)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     promoter        complement(1415..1506)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(1473..1490)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     enhancer        1539..1918
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1919..2122
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2073..2093
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2119..2143
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2147..2279
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2262..2281
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             2320..2337
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    2344..2932
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2397..2417)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2815..2826)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3059..3193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3096..3115)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3150..3169
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        join(3592..3597,3598..3614,3615..3620)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3790..4323
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  4390..4614
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(4748..4765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4919..5507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4999..5018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5678..6469,6470..6538))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6301..6320
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6539..6643)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6669..7123)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6806..6827)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7017..7036
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa
       61 cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg aagatgacgg
      121 tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga aaaatgcccc
      181 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt
      241 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa
      301 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg gatctcctag
      361 gctcaagcag tgatcagtgg ctatggcagg gcttgccgcc ccgacgttgg ctgcgagccc
      421 tgggccttca cccgaacttg ggggttgggg tggggaaaag gaagaaacgc gggcgtattg
      481 gtcccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
      541 tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag
      601 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc catctcccgc
      661 tctagtactt ctcgacaagc ttttaggcca gggctttacc ctcggtcttg gaggtgacgt
      721 ccacctggac acccacgaac ttggacacgc gaccgtcggg agtcttgatg ggggtcacag
      781 tcagcaggtt ccagaaaggg gtaccgtcct tgcggtagtt cagcagacgc acggagcagg
      841 cttcaccctt cttgatagcg tcgcggatct tctgcacctc cttggggtcg gtaccttcac
      901 cctgcaggaa gcgggcgttg tggcccagca cttcgtcggg gccgtaacca gtcatggcgt
      961 agaagccctc ggaagcgtac accagggggc agtcgggcag agtagcgtca gccaccacga
     1021 aggtgtgacg cagaccggca ccgccacctt tacccaggcg gttcatctcg atgtcagtat
     1081 atatctgacg cttcttggtg tgcttcagct tgatgcacag gtggataccc acgcgcagga
     1141 ccaggaacag accgatgatc aggccgatga tgaagaagaa agaagcgatg ctggacttcc
     1201 agctgctgaa ccagccttcg accagttcga tagggttctt gctcagaccg gtgtcaccag
     1261 cgaaggcgga gtgagcagca gcagccagca gcacgtacag cacgatagcg ctcaccatct
     1321 tgctagtgtg ctccttgttg aaaccctggt ggctctggtt caccagcagc atagatccgc
     1381 gcccgatggt gggacggtat gaataatccg gaatatttat aggttttttt attacaaaac
     1441 tgttacgaaa acagtaaaat acttatttat ttgcgagatg gttatcattt taattatctc
     1501 catgatctat taatacttaa ttaacaacac catttgttgg cattgattat tgactagtta
     1561 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     1621 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     1681 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     1741 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc
     1801 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     1861 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     1921 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc
     1981 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     2041 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg
     2101 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcaaggtaa gtatcaaggt
     2161 tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt
     2221 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg
     2281 tgtaaggaat tcggatcccg ggcggccgct agcctcgagc accaccacca ccaccactga
     2341 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2401 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2461 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2521 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2581 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2641 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2701 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     2761 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     2821 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     2881 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtcga
     2941 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     3001 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3061 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3121 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3181 tcatgtctgg atctgatcag taacaacacc atttaaatgg agtggttaca aatggagtgg
     3241 ttaattaagt tccggagtat acgtagccaa ccactagaac tatagctaga gtcctgggcg
     3301 aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc
     3361 accaccggca agcgccgcga cggccgaggt cttccgatct cctgaagcca gggcagatcc
     3421 gtgcacagca ccttgccgta gaagaacagc aaggccgcca atgcctgacg atgcgtggag
     3481 accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc
     3541 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gttgacataa
     3601 gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     3661 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     3721 tgtttttttg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     3781 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     3841 aagttaggtg gctcaagtat gggcatcatt cgcacatgta ggctcggccc tgaccaagtc
     3901 aaatccatgc gggctgctct tgatcttttc ggtcgtgagt tcggagacgt agccacctac
     3961 tcccaacatc agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatc
     4021 gcgcttgctg ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc
     4081 aagtttgagc agccgcgtag tgagatctat atctatgatc tcgcagtctc cggcgagcac
     4141 cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt
     4201 ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat
     4261 acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc
     4321 taacaattcg ttcaagccga gatcggcttc ccggccgcgg agttgttcgg taaattgtca
     4381 caacgccgcg aatatagtct ttaccatgcc cttggccacg cccctcttta atacgacggg
     4441 caatttgcac ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt
     4501 tttcacagca taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta
     4561 ttgtcatagt ttagatctat tttgttcagt ttaagacttt attgtccgcc cacacccgct
     4621 tacgcagggc atccatttat tactcaaccg taaccgattt tgccaggtta cgcggctggt
     4681 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     4741 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4801 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4861 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4921 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4981 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5041 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5101 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5161 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5221 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5281 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5341 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5401 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5461 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5521 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5581 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5641 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5701 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5761 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5821 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5881 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5941 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6001 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6061 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6121 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6181 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6241 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6301 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6361 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6421 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6481 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6541 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6601 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6661 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     6721 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     6781 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taagaacgtg
     6841 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     6901 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     6961 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     7021 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     7081 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtc
//
