LOCUS       sequence-349611-       20403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) 
            but with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20403)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D.
  JOURNAL   Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9.
  PUBMED    34824475
REFERENCE   2  (bases 1 to 20403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9."
COMMENT     Sequence Label: YFVdelNS1-mCherry
FEATURES             Location/Qualifiers
     source          1..20403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    8..41
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    59..457
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    717..1190
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(1263..2234)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(2234..3409)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    3735..3985
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3988..4743)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      4834..5053
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      5121..5652
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5129..5743
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        6602..6704
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6705..7364
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6771..6790)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    7584..7605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7620..7625,7626..7643,7644..7650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7658..7674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7663..7685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7682..7698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7682..7698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8135..8153
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8135..8152
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             8352..9059
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8497..8515)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8765..8784
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8959..8982
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     19693..19712
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(20076..20095)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(20077..20095)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(20102..20119)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(20102..20118)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(20111..20133)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(20326..20345)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atcgaatata acttcgtata atgtatgcta tacgaagtta ttagcgatga gctcggactt
       61 ccattgttca ttccacggac aaaaacagag aaaggaaacg acagaggcca aaaagctcgc
      121 tttcagcacc tgtcgtttcc tttcttttca gagggtattt taaataaaaa cattaagtta
      181 tgacgaagaa gaacggaaac gccttaaacc ggaaaatttt cataaatagc gaaaacccgc
      241 gaggtcgccg ccccgtaacc tgtcggatca ccggaaagga cccgtaaagt gataatgatt
      301 atcatctaca tatcacaacg tgcgtggagg ccatcaaacc acgtcaaata atcaattatg
      361 acgcaggtat cgtattaatt gatctgcatc aacttaacgt aaaaacaact tcagacaata
      421 caaatcagcg acactgaata cggggcaacc tcatgtccga gctcgcgagc tcgtcgacag
      481 cgacacactt gcatcggatg cagcccggtt aacgtgccgg cacggcctgg gtaaccaggt
      541 attttgtcca cataaccgtg cgcaaaatgt tgtggataag caggacacag cagcaatcca
      601 cagcaggcat acaaccgcac accgaggtta ctccgttcta caggttacga cgacatgtca
      661 atacttgccc ttgacaggca ttgatggaat cgtagtctca cgctgatagt ctgatcgaca
      721 atacaagtgg gaccgtggtc ccagaccgat aatcagaccg acaacacgag tgggatcgtg
      781 gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag actaataatc
      841 agaccgacga tacgagtggg accgtggttc cagactaata atcagaccga cgatacgagt
      901 gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggacca tggtcccaga
      961 ctaataatca gaccgacgat acgagtggga ccgtggtccc agtctgatta tcagaccgac
     1021 gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt
     1081 ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca gtctgattat
     1141 cagaccgacg atacaagtgg aacagtgggc ccagagagaa tattcaggcc agttatgctt
     1201 tctggcctgt aacaaaggac attaagtaaa gacagataaa cgtagactaa aacgtggtcg
     1261 catcagggtg ctggcttttc aagttcctta agaatggcct caattttctc tatacactca
     1321 gttggaacac gagacctgtc caggttaagc accattttat cgcccttata caatactgtc
     1381 gctccaggag caaactgatg tcgtgagctt aaactagttc ttgatgcaga tgacgtttta
     1441 agcacagaag ttaaaagagt gataacttct tcagcttcaa atatcacccc agcttttttc
     1501 tgctcatgaa ggttagatgc ctgctgctta agtaattcct ctttatctgt aaaggctttt
     1561 tgaagtgcat cacctgaccg ggcagatagt tcaccggggt gagaaaaaag agcaacaact
     1621 gatttaggca atttggcggt gttgatacag cgggtaataa tcttacgtga aatattttcc
     1681 gcatcagcca gcgcagaaat atttccagca aattcattct gcaatcggct tgcataacgc
     1741 tgaccacgtt cataagcact tgttgggcga taatcgttac ccaatctgga taatgcagcc
     1801 atctgctcat catccagctc gccaaccaga acacgataat cactttcggt aagtgcagca
     1861 gctttacgac ggcgactccc atcggcaatt tctatgacac cagatactct tcgaccgaac
     1921 gccggtgtct gttgaccagt cagtagaaaa gaagggatga gatcatccag tgcgtcctca
     1981 gtaagcagct cctggtcacg ttcattacct gaccataccc gagaggtctt ctcaacacta
     2041 tcaccccgga gcacttcaag agtaaacttc acatcccgac cacatacagg caaagtaatg
     2101 gcattaccgc gagccattac tcctacgcgc gcaattaacg aatccaccat cggggcagct
     2161 ggtgtcgata acgaagtatc ttcaaccggt tgagtattga gcgtatgttt tggaataaca
     2221 ggcgcacgct tcattatcta atctcccagc gtggtttaat cagacgatcg aaaatttcat
     2281 tgcagacagg ttcccaaata gaaagagcat ttctccaggc accagttgaa gagcgttgat
     2341 caatggcctg ttcaaaaaca gttctcatcc ggatctgacc tttaccaact tcatccgttt
     2401 cacgtacaac attttttaga accatgcttc cccaggcatc ccgaatttgc tcctccatcc
     2461 acggggactg agagccattg ctattgctgt atttggtaag caaaatacgt acatcaggct
     2521 cgaacccttt aagatcaacg ttcttgagca gatcacgaag catatcgaaa aactgcagtg
     2581 cggaggtgta gtcaaacaac tcagcaggcg tgggaacaat cagcacatca gcagcacata
     2641 cgacattaat cgtgccgata cccaggttag gcgcgctgtc aataactatg acatcatagt
     2701 catgagcaac agtttcaatg gccagtcgga gcatcaggtg tggatcggtg ggcagtttac
     2761 cttcatcaaa tttgcccatt aactcagttt caatacggtg cagagccaga caggaaggaa
     2821 taatgtcaag ccccggccag caagtgggct ttattgcata agtgacatcg tccttttccc
     2881 caagatagaa aggcaggaga gtgtcttctg catgaatatg aagatctggt acccatccgt
     2941 gatacattga ggctgttccc tgggggtcgt taccttccac gagcaaaaca cgtagcccct
     3001 tcagagccag atcctgagca agatgaacag aaactgaggt tttgtaaacg ccacctttat
     3061 gggcagcaac cccgatcacc ggtggaaata cgtcttcagc acgtcgcaat cgcgtaccaa
     3121 acacatcacg catatgatta atttgttcaa ttgtataacc aacacgttgc tcaacccgtc
     3181 ctcgaatttc catatccggg tgcggtagtc gccctgcttt ctcggcatct ctgatagcct
     3241 gagaagaaac cccaactaaa tccgctgctt cacctattct ccagcgccgg gttattttcc
     3301 tcgcttccgg gctgtcatca ttaaactgtg caatggcgat agccttcgtc atttcatgac
     3361 cagcgtttat gcactggtta agtgtttcca tgagtttcat tctgaacatc ctttaatcat
     3421 tgctttgcgt ttttttatta aatcttgcaa tttactgcaa agcaacaaca aaatcgcaaa
     3481 gtcatcaaaa aaccgcaaag ttgtttaaaa taagagcaac actacaaaag gagataagaa
     3541 gagcacatac ctcagtcact tattatcact agcgctcgcc gcagccgtgt aaccgagcat
     3601 agcgagcgaa ctggcgagga agcaaagaag aactgttctg tcagatagct cttacgctca
     3661 gcgcaagaag aaatatccac cgtgggaaaa actccaggta gaggtacaca cgcggatagc
     3721 caattcagag taataaactg tgataatcaa ccctcatcaa tgatgacgaa ctaacccccg
     3781 atatcaggtc acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa ctgccctcaa
     3841 atttggcttc cttaaaaatt acagttcaaa aagtatgaga aaatccatgc aggctgaagg
     3901 aaacagcaaa actgtgacaa attaccctca gtaggtcaga acaaatgtga cgaaccaccc
     3961 tcaaatctgt gacagataac cctcagacta tcctgtcgtc atggaagtga tatcgcggaa
     4021 ggaaaatacg atatgagtcg tctggcggcc tttctttttc tcaatgtatg agaggcgcat
     4081 tggagttctg ctgttgatct cattaacaca gacctgcagg aagcggcggc ggaagtcagg
     4141 catacgctgg taactttgag gcagctggta acgctctatg atccagtcga ttttcagaga
     4201 gacgatgcct gagccatccg gcttacgata ctgacacagg gattcgtata aacgcatggc
     4261 atacggattg gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc ggttctgtaa
     4321 cccgataaag aagggaatga gatatgggtt gatatgtaca ctgtaaagcc ctctggatgg
     4381 actgtgcgca cgtttgataa accaaggaaa agattcatag cctttttcat cgccggcatc
     4441 ctcttcaggg cgataaaaaa ccacttcctt ccccgcgaaa ctcttcaatg cctgccgtat
     4501 atccttactg gcttccgcag aggtcaatcc gaatatttca gcatatttag caacatggat
     4561 ctcgcagata ccgtcatgtt cctgtagggt gccatcagat tttctgatct ggtcaacgaa
     4621 cagatacagc atacgttttt gatcccggga gagactatat gccgcctcag tgaggtcgtt
     4681 tgactggacg attcgcgggc tatttttacg tttcttgtga ttgataaccg ctgtttccgc
     4741 catgacagat ccatgtgaag tgtgacaagt ttttagattg tcacactaaa taaaaaagag
     4801 tcaataagca gggataactt tgtgaaaaaa cagcttcttc tgagggcaat ttgtcacagg
     4861 gttaagggca atttgtcaca gacaggactg tcatttgagg gtgatttgtc acactgaaag
     4921 ggcaatttgt cacaacacct tctctagaac cagcatggat aaaggcctac aaggcgctct
     4981 aaaaaagaag atctaaaaac tataaaaaaa ataattataa aaatatcccc gtggataagt
     5041 ggataacccc aagggaagtt ttttcaggca tcgtgtgtaa gcagaatata taagtgctgt
     5101 tccctggtgc ttcctcgctc actcgaccgg gagggttcga gaaggggggg cacccccctt
     5161 cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat
     5221 tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt
     5281 gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca
     5341 tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc
     5401 gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg
     5461 gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc
     5521 cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca
     5581 agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca
     5641 acgccggcgg ccggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg
     5701 cagggccata gacggccgcc agcccagcgg cgagggcaac cagctcgagg gcttcgccct
     5761 gtcgctcgac tgcggcgagc actactggct gtaaaaggac agaccacatc atggttctgt
     5821 gttcattagg ttgttctgtc cattgctgac ataatccgct ccacttcaac gtaacaccgc
     5881 acgaagattt ctattgttcc tgaaggcata ttcaaatcgt tttcgttacc gcttgcaggc
     5941 atcatgacag aacactactt cctataaacg ctacacaggc tcctgagatt aataatgcgg
     6001 atctctacga taatgggaga ttttcccgac tgtttcgttc gcttctcagt ggataacagc
     6061 cagcttctct gtttaacaga caaaaacagc atatccactc agttccacat ttccatataa
     6121 aggccaaggc atttattctc aggataattg tttcagcatc gcaaccgcat cagactccgg
     6181 catcgcaaac tgcacccggt gccgggcagc cacatccagc gcaaaaacct tcgtgtagac
     6241 ttccgttgaa ctgatggact tatgtcccat caggctttgc agaactttca gcggtatacc
     6301 ggcatacagc atgtgcatcg cataggaatg gcggaacgta tgtggtgtga ccggaacaga
     6361 gaacgtcaca ccgtcagcag cagcggcggc aaccgcctcc ccaatccagg tcctgaccgt
     6421 tctgtccgtc acttcccaga tccgcgcttt ctctgtcctt cctgtgcgac ggttacgccg
     6481 ctccatgagc ttatcgcgaa taaatacctg tgacggaaga tcacttcgca gaataaataa
     6541 atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg
     6601 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     6661 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     6721 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     6781 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     6841 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     6901 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     6961 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     7021 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     7081 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     7141 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     7201 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     7261 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     7321 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
     7381 attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg
     7441 gcagaaattc gatgataagc tgtcaaacat gagaattggt cgacggcccg ggcggccgca
     7501 aggagttcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7561 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7621 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7681 acaggaaaca gctatgacca tgattacgcc aagctattta ggtgagacta tagaatactc
     7741 aagcttgcat gcctgcaggt cgactctaga ggatcccaca ttattgaagc atttatcagg
     7801 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7861 ttccgcgcac atttccccga aaagtgccac ctgacgtgtc gacgcggccg ctagcgatga
     7921 ccctgctgat tggttcgctg accatttccg ggtgcgggac ggcgttacca gaaactcaga
     7981 aggttcgtcc aaccaaaccg actctgacgg cagtttacga gagagatgat agggtctgct
     8041 tcagtaagcc agatgctaca caattaggct tgtacatatt gtcgttagaa cgcggctaca
     8101 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agagtaaatc
     8161 ctgtgtgcta attgaggtgc attggtctgc aaatcgagtt gctaggcaat aaacacattt
     8221 ggattaattt taatcgttcg ttgagcgatt agcagagaac tgaccagaac atgtctggtc
     8281 gtaaagctca gggaaaaacc ctgggcgtca atatggtacg acgaggagtt cgctccttgt
     8341 caaacgagct catggtgagc aagggcgagg aggacaacat ggccatcatc aaggagttca
     8401 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     8461 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
     8521 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     8581 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     8641 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     8701 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     8761 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     8821 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     8881 actacgacgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     8941 cctacaacgt caacatcaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     9001 aacagtacga gcgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaaga
     9061 ccggtaactt tgaccttctc aagttggccg gcgacgtcga gtccaaccca gggcccctgc
     9121 agcaaatttt cgtgaagacc ctgacgggca agaccatcac tcttgaggtc gagcccagtg
     9181 acaccatcga gaatgtcaag gccaagatcc aagacaagga aggcatccca cctgaccagc
     9241 agaggctgat attcgcgggc aaacagctgg aggatggccg caccctgtcc gactacaaca
     9301 tccagaaaga gtccaccttg cacctggtgc tgtgtctccg cggtggaatg tccggtcgta
     9361 aagcccaggg caaaacccta ggcgtaaata tggtccgacg cggagtccgc tcattgtcga
     9421 acaaaataaa acaaaaaaca aaacaaattg gaaacagacc tggaccttca agaggtgttc
     9481 aaggatttat ctttttcttt ttgttcaaca ttttgactgg aaaaaagatc acagcccacc
     9541 taaagaggtt gtggaaaatg ctggacccaa gacaaggctt ggctgttcta aggaaagtca
     9601 agagagtggt ggccagtttg atgagaggat tgtcctcaag gaaacgccgt tcccatgatg
     9661 ttctgactgt gcaattccta attttgggaa tgctgttgat gacgggtgga gtgaccttgg
     9721 tgcgnaaaaa cagatggttg ctcctaaatg tgacatctga ggacctcggg aaaacattct
     9781 ctgtgggcac aggcaactgc acaacaaaca ttttggaagc caagtactgg tgcccagact
     9841 caatggaata caactgtccc aatctcagtc caagagagga gccagatgac attgattgct
     9901 ggtgctatgg ggtggaaaac gttagagtcg catatggtaa gtgtgactca gcaggcaggt
     9961 ctaggaggtc aagaagggcc attgacttgc ctacgcatga aaaccatggt ttgaagaccc
    10021 ggcaagaaaa atggatgact ggaagaatgg gtgaaaggca actccaaaag attgagagat
    10081 ggttcgtgag gaaccccttt tttgcagtga cggctctgac cattgcctac cttgtgggaa
    10141 gcaacatgac gcaacgagtc gtgattgccc tactggtctt ggctgttggt ccggcctact
    10201 cagctcactg cattggaatt actgacaggg atttcattga gggggtgcat ggaggaactt
    10261 gggtttcagc taccctggag caagacaagt gtgtcactgt tatggcccct gacaagcctt
    10321 cattggacat ctcactagag acagtagcca ttgatagacc tgctgaggtg aggaaagtgt
    10381 gttacaatgc agttctcact catgtgaaga ttaatgacaa gtgccccagc actggagagg
    10441 cccacctagc cgaagagaac gaaggggaca atgcgtgcaa gcgcacttat tctgatagag
    10501 gctggggcaa tggctgtggc ctatttggga aagggagcat tgtggcatgc gccaaattca
    10561 cttgtgccaa atccatgagt ttgtttgagg ttgatcagac caaaattcag tatgtcatca
    10621 gagcacaatt gcatgtaggg gccaagcagg aaaattggaa taccgacatt aagactctca
    10681 agtttgatgc cctgtcaggc tcccaggaag tcgagttcat tgggtatgga aaagctacac
    10741 tggaatgcca ggtgcaaact gcggtggact ttggtaacag ttacatcgct gagatggaaa
    10801 cagagagctg gatagtggac agacagtggg cccaggactt gaccctgcca tggcagagtg
    10861 gaagtggcgg ggtgtggaga gagatgcatc atcttgtcga atttgaacct ccgcatgccg
    10921 ccactatcag agtactggcc ctgggaaacc aggaaggctc cttgaaaaca gctcttactg
    10981 gcgcaatgag ggttacaaag gacacaaatg acaacaacct ttacaaacta catggtggac
    11041 atgtttcttg cagagtgaaa ttgtcagctt tgacactcaa ggggacatcc tacaaaatat
    11101 gcactgacaa aatgtttttt gtcaagaacc caactgacac tggccatggc actgttgtga
    11161 tgcaggtgaa agtgtcaaaa ggagccccct gcaggattcc agtgatagta gctgatgatc
    11221 ttacagcggc aatcaataaa ggcattttgg ttacagttaa ccccatcgcc tcaaccaatg
    11281 atgatgaagt gctgattgag gtgaacccac cttttggaga cagctacatt atcgttggga
    11341 gaggagattc acgtctcact taccagtggc acaaagaggg aagctcaata ggaaagttgt
    11401 tcactcagac catgaaaggc gtggaacgcc tggccgtcat gggagacacc gcctgggatt
    11461 tcagctccgc tggagggttc ttcacttcgg ttgggaaagg aattcatacg gtgtttggct
    11521 ctgcctttca ggggctattt ggcggcttga actggataac aaaggtcatc atgggggcgg
    11581 tacttatatg ggttggcatc aacacaagaa acatgacaat gtccatgagc atgatcttgg
    11641 taggagtgat catgatgttt ttgtctctag gagttggggc ggatcaagga tgcgccatca
    11701 actttggcaa gagagagctc ggatcacggt ccgggacacg cgtgtacatg gacgcagtct
    11761 ttgaatacac catagactgc gatggatcta tcttgggtgc agcggtgaac ggaaaaaaga
    11821 gtgcccatgg ctctccaaca ttttggatgg gaagtcatga agtaaatggg acatggatga
    11881 tccacacctt ggaggcatta gattacaagg agtgtgagtg gccactgaca catacgattg
    11941 gaacatcagt tgaagagagt gaaatgttca tgccgagatc aatcggaggc ccagttagct
    12001 ctcacaatca tatccctgga tacaaggttc agacgaacgg accttggatg caggtaccac
    12061 tagaagtgaa gagagaagct tgcccaggga ctagcgtgat cattgatggc aactgtgatg
    12121 gacggggaaa atcaaccaga tccaccacgg atagcgggaa agttattcct gaatggtgtt
    12181 gccgctcctg cacaatgccg cctgtgagct tccatggtag tgatgggtgt tggtatccca
    12241 tggaaattag gccaaggaaa acgcatgaaa gccatctggt gcgctcctgg gttacagctg
    12301 gagaaataca tgctgtccct tttggtttgg tgagcatgat gatagcaatg gaagtggtcc
    12361 taaggaaaag acagggacca aagcaaatgt tggttggagg agtagtgctc ttgggagcaa
    12421 tgctggtcgg gcaagtaact ctccttgatt tgctgaaact cacagtggct gtgggattgc
    12481 atttccatga gatgaacaat ggaggagacg ccatgtatat ggcgttgatt gctgcctttt
    12541 caatcagacc agggctgctc atcggctttg ggctcaggac cctatggagc cctcgggaac
    12601 gccttgtgct gaccctagga gcagccatgg tggagattgc cttgggtggc gtgatgggcg
    12661 gcctgtggaa gtatctaaat gcagtttctc tctgcatcct gacaataaat gctgttgctt
    12721 ctaggaaagc atcaaatacc atcttgcccc tcatggctct gttgacacct gtcactatgg
    12781 ctgaggtgag acttgccgca atgttctttt gtgccatggt tatcataggg gtccttcacc
    12841 agaatttcaa ggacacctcc atgcagaaga ctatacctct ggtggccctc acactcacat
    12901 cttacctggg cttgacacaa ccttttttgg gcctgtgtgc atttctggca acccgcatat
    12961 ttgggcgaag gagtatccca gtgaatgagg cactcgcagc agctggtcta gtgggagtgc
    13021 tggcaggact ggcttttcag gagatggaga acttccttgg tccgattgca gttggaggac
    13081 tcctgatgat gctggtatgc gtggctggga gggtggatgg gctagagctc aagaagcttg
    13141 gtgaagtttc atgggaagag gaggcggaga tcagcgggag ttccgcccgc tatgatgtgg
    13201 cactcagtga acaaggggag ttcaagctgc tttctgaaga gaaagtgcca tgggaccagg
    13261 ttgtgatgac ctcgctggcc ttggttgggg ctgccctcca tccatttgct cttctgctgg
    13321 tccttgctgg gtggctgttt catgtcaggg gagcgcgcag aagtggtcac cggagaagtg
    13381 gggatgtctt gtgggatatt cccactccta agatcatcga ggaatgtgaa catctggagg
    13441 atgggattta tggcatattc cagtcaacct tcttgggggc ctcccagcga ggagtgggag
    13501 tggcacaggg aggggtgttc cacacaatgt ggcatgtcac aagaggagct ttccttgtca
    13561 ggaatggcaa gaagttgatt ccatcttggg cttcagtaaa ggaagacctt gtcgcctatg
    13621 gtggctcatg gaagttggaa ggcagatggg atggagagga agaggtccag ttgatcgcgg
    13681 ctgttccagg aaagaacgtg gtcaacgtcc agacaaaacc gagcttgttc aaagtgagga
    13741 atgggggaga aatcggggct gtcgctcttg actatccgag tggcacttca ggatctccta
    13801 ttgttaacag gaacggagag gtgattgggc tgtacggcaa tggcatcctt gtcggtgaca
    13861 actccttcgt gtccgccata tcccagactg aggtgaagga agaaggaaag gaggagctcc
    13921 aagagatccc gacaatgcta aagaaaggaa tgacaactgt ccttgatttt catcctggag
    13981 ctgggaagac aagacgtttc ctcccacaga tcttggccga gtgcgcacgg agacgcttgc
    14041 gcactcttgt gttggccccc accagggttg ttctttctga aatgaaggag gcttttcacg
    14101 gcctggacgt gaaattccac acacaggctt tttccgctca cggcagcggg agagaagtca
    14161 ttgatgccat gtgccatgcc accctaactt acaggatgtt ggaaccaact agggttgtta
    14221 actgggaagt gatcattatg gatgaagccc attttttgga tccagctagc atagccgcta
    14281 gaggttgggc agcgcacaga gctagggcaa atgaaagtgc aacaatcttg atgacagcca
    14341 caccgcctgg gactagtgat gaatttccac attcaaatgg tgaaatagaa gatgttcaaa
    14401 cggacatacc cagtgagccc tggaacacag ggcatgactg gatcctggct gacaaaaggc
    14461 ccacggcatg gttccttcca tccatcagag ctgcaaatgt catggctgcc tctttgcgta
    14521 aggctggaaa gagtgtggtg gtcctgaaca ggaaaacctt tgagagagaa taccccacga
    14581 taaagcagaa gaaacctgac tttatattgg ccactgacat agctgaaatg ggagccaacc
    14641 tttgcgtgga gcgagtgctg gattgcagga cggcttttaa gcctgtgctt gtggatgaag
    14701 ggaggaaggt ggcaataaaa gggccacttc gtatctccgc atcctctgct gctcaaagga
    14761 gggggcgcat tgggagaaat cccaacagag atggagactc atactactat tctgagccta
    14821 caagtgaaaa taatgcccac cacgtctgct ggttggaggc ctcaatgctc ttggacaaca
    14881 tggaggtgag gggtggaatg gtcgccccac tctatggcgt tgaaggaact aaaacaccag
    14941 tttcccctgg tgaaatgaga ctgagggatg accagaggaa agtcttcaga gaactagtga
    15001 ggaattgtga cctgcccgtt tggctttcgt ggcaagtggc caaggctggt ttgaagacga
    15061 atgatcgtaa gtggtgtttt gaaggccctg aggaacatga gatcttgaat gacagcggtg
    15121 aaacagtgaa gtgcagggct cctggaggag caaagaagcc tctgcgccca aggtggtgtg
    15181 atgaaagggt gtcatctgac cagagtgcgc tgtctgaatt tattaagttt gctgaaggta
    15241 ggaggggagc tgctgaagtg ctagttgtgc tgagtgaact ccctgatttc ctggctaaaa
    15301 aaggtggaga ggcaatggat accatcagtg tgttcctcca ctctgaggaa ggctctaggg
    15361 cttaccgcaa tgcactatca atgatgcctg aggcaatgac aatagtcatg ctgtttatac
    15421 tggctggact actgacatcg ggaatggtca tctttttcat gtctcccaaa ggcatcagta
    15481 gaatgtctat ggcgatgggc acaatggccg gctgtggata tctcatgttc cttggaggcg
    15541 tcaaacccac tcacatctcc tatgtcatgc tcatattctt tgtcctgatg gtggttgtga
    15601 tccccgagcc agggcaacaa aggtccatcc aagacaacca agtggcatac ctcattattg
    15661 gcatcctgac gctggtttca gcggtggcag ccaacgagct aggcatgctg gagaaaacca
    15721 aagaggacct ctttgggaag aagaacttaa ttccatctag tgcttcaccc tggagttggc
    15781 cggatcttga cctgaagcca ggagctgcct ggacagtgta cgttggcatt gttacaatgc
    15841 tctctccaat gttgcaccac tggatcaaag tcgaatatgg caacctgtct ctgtctggaa
    15901 tagcccagtc agcctcagtc ctttctttca tggacaaggg gataccattc atgaagatga
    15961 atatctcggt cataatgctg ctggtcagtg gctggaattc aataacagtg atgcctctgc
    16021 tctgtggcat agggtgcgcc atgctccact ggtctctcat tttacctgga atcaaagcgc
    16081 agcagtcaaa gcttgcacag agaagggtgt tccatggcgt tgccaagaac cctgtggttg
    16141 atgggaatcc aacagttgac attgaggaag ctcctgaaat gcctgccctt tatgagaaga
    16201 aactggctct atatctcctt cttgctctca gcctagcttc tgttgccatg tgcagaacgc
    16261 ccttttcatt ggctgaaggc attgtcctag catcagctgc cttagggccg ctcatagagg
    16321 gaaacaccag ccttctttgg aatggaccca tggctgtctc catgacagga gtcatgaggg
    16381 ggaatcacta tgcttttgtg ggagtcatgt acaatctatg gaagatgaaa actggacgcc
    16441 gggggagcgc gaatggaaaa actttgggtg aagtctggaa gagggaactg aatctgttgg
    16501 acaagcgaca gtttgagttg tataaaagga ccgacattgt ggaggtggat cgtgatacgg
    16561 cacgcaggca tttggccgaa gggaaggtgg acaccggggt ggcggtctcc agggggaccg
    16621 caaagttaag gtggttccat gagcgtggct atgtcaagct ggaaggtagg gtgattgacc
    16681 tggggtgtgg ccgcggaggc tggtgttact acgctgctgc gcaaaaggaa gtgagtgggg
    16741 tcaaaggatt tactcttgga agagacggcc atgagaaacc catgaatgtg caaagtctgg
    16801 gatggaacat catcaccttc aaggacaaaa ctgatatcca ccgcctagaa ccagtgaaat
    16861 gtgacaccct tttgtgtgac attggagagt catcatcgtc atcggtcaca gagggggaaa
    16921 ggaccgtgag agttcttgat actgtagaaa aatggctggc ttgtggggtt gacaacttct
    16981 gtgtgaaggt gttagctcca tacatgccag atgttcttga gaaactggaa ttgctccaaa
    17041 ggaggtttgg cggaacagtg atcaggaacc ctctctccag gaattccact catgaaatgt
    17101 actacgtgtc tggagcccgc agcaatgtca catttactgt gaaccaaaca tcccgcctcc
    17161 tgatgaggag aatgaggcgt ccaactggaa aagtgaccct ggaggctgac gtcatcctcc
    17221 caattgggac acgcagtgtt gagacagaca agggacccct ggacaaagag gccatagaag
    17281 aaagggttga gaggataaaa tctgagtaca tgacctcttg gttttatgac aatgacaacc
    17341 cctacaggac ctggcactac tgtggctcct atgtcacaaa aacctcagga agtgcggcga
    17401 gcatggtaaa tggtgttatt aaaattctga catatccatg ggacaggata gaggaggtca
    17461 caagaatggc aatgactgac acaacccctt ttggacagca aagagtgttt aaagaaaaag
    17521 ttgacaccag agcaaaggat ccaccagcgg gaactaggaa gatcatgaaa gttgtcaaca
    17581 ggtggctgtt ccgccacctg gccagagaaa agaaccccag actgtgcaca aaggaagaat
    17641 ttattgcaaa agtccgaagt catgcagcca ttggagctta cctggaagaa caagaacagt
    17701 ggaagactgc caatgaggct gtccaagacc caaagttctg ggaactggtg gatgaagaaa
    17761 ggaagctgca ccaacaaggc aggtgtcgga cttgtgtgta caacatgatg gggaaaagag
    17821 agaagaagct gtcagagttt gggaaagcaa agggaagccg tgccatatgg tatatgtggc
    17881 tgggagcgcg gtatcttgag tttgaggccc tgggattcct gaatgaggac cattgggctt
    17941 ccagggaaaa ctcaggagga ggagtggaag gcattggctt acaataccta ggatatgtga
    18001 tcagagacct ggctgcaatg gatggtggtg gattctacgc ggatgacacc gctggatggg
    18061 acacgcgcat cacagaggca gaccttgatg atgaacagga gatcttgaac tacatgagcc
    18121 cacatcacaa aaaactggca caagcagtga tggaaatgac atacaagaac aaagtggtga
    18181 aagtgttgag accagcccca ggagggaaag cctacatgga tgtcataagt cgacgagacc
    18241 agagaggatc cgggcaggta gtgacttatg ctctgaacac catcaccaac ttgaaagtcc
    18301 aattgatcag aatggcagaa gcagagatgg tgatacatca ccaacatgtt caagattgtg
    18361 atgaatcagt tctgaccagg ctggaggcat ggctcactga gcacggatgt gacagactga
    18421 agaggatggc ggtgagtgga gacgactgtg tggtccggcc catcgatgac aggttcggcc
    18481 tggccctgtc ccatctcaac gccatgtcca aggttagaaa ggacatatct gaatggcagc
    18541 catcaaaagg gtggaatgat tgggagaatg tgcccttctg ttcccaccac ttccatgaac
    18601 tacagctgaa ggatggcagg aggattgtgg tgccttgccg agaacaggac gagctcattg
    18661 ggagaggaag ggtgtctcca ggaaacggct ggatgatcaa ggaaacagct tgcctcagca
    18721 aagcctatgc caacatgtgg tcactgatgt attttcacaa aagggacatg aggctactgt
    18781 cattggctgt ttcctcagct gttcccacct catgggttcc acaaggacgc acaacatggt
    18841 cgattcatgg gaaaggggag tggatgacca cggaagacat gcttgaggtg tggaacagag
    18901 tatggataac caacaaccca cacatgcagg acaagacaat ggtgaaaaaa tggagagatg
    18961 tcccttatct aaccaagaga caagacaagc tgtgcggatc actgattgga atgaccaata
    19021 gggccacctg ggcctcccac atccatttag tcatccatcg tatccgaacg ctgattggac
    19081 aggagaaata cactgactac ctaacagtca tggacaggta ttctgtggat gctgacctgc
    19141 aactgggtga gcttatctga aacaccatct aacaggaata accgggatac aaaccacggg
    19201 tggagaaccg gactccccac aacctgaaac cgggatataa accacggctg gagaaccgga
    19261 ctccgcactt aaaatgaaac agaaaccggg ataaaaacta cggatggaga accggactcc
    19321 acacattgag acagaagaag ttgtcagccc agaaccccac acgagttttg ccactgctaa
    19381 gctgtgaggc agtgcaggct gggacagccg acctccaggt tgcgaaaaac ctggtttctg
    19441 ggacctccca ccccagagta aaaagaacgg agcctccgct accaccctcc cacgtggtgg
    19501 tagaaagacg gggtctagag gttagaggag accctccagg gaacaaatag tgggaccata
    19561 ttgacgccag ggaaagaccg gagtggttct ctgcttttcc tccagaggtc tgtgagcaca
    19621 gtttgctcaa gaataagcag acctttggat gacaaacaca aaaccactcg agcaagacgt
    19681 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
    19741 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
    19801 aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct
    19861 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac
    19921 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat
    19981 tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc gctagcggag
    20041 tgtagtggga tccccgggta ccgagctcga attcgcccta tagtgagtcg tattacaatt
    20101 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    20161 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    20221 gcccttccca acagttgcgc agctgaatgg cgaatggcgc ctgatgcggt attttctcct
    20281 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
    20341 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcga accccttgcg
    20401 gcc
//
