LOCUS       sequence-349617-        3411 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector 
            for encoding a human codon-optimized SiCas12i driven by CBh promoter
            and tagmCherry driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Zhang H, Kong X, Xue M, Hu J, Wang Z, Wei Y, Wang H, Zhou J, Zhang 
            W, Xu M, Shen X, Yin F, Ai Z, Huang G, Xia J, Song X, Li H, Yuan Y, 
            Li J, Zhong N, Zhang M, Zhou Y, Yang H
  TITLE     An engineered xCas12i with high activity, high specificity, and 
            broad PAM range.
  JOURNAL   Protein Cell. 2023 Jun 28;14(7):538-543. doi: 
            10.1093/procel/pwac052.
  PUBMED    37378659
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Protein Cell"; date: "2023-06-28- 28"; volume: "14"; issue: "7"; 
            pages: "538-543"
COMMENT     Sequence Label: HEpl016
FEATURES             Location/Qualifiers
     source          1..3411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3361..3408
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 atggactata aggaccacga cggagactac aaggatcatg atattgatta caaagacgat
       61 gacgataaga tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc
      121 atgagttctg atgtggtgcg gccttataac acaaagctgc tcccagataa cagaaagcac
      181 aatatgttcc tgcagacctt caagcggctg aacagcatct ctctgaacca cttcgacctg
      241 ctgatctgcc tgtacgctgc aatcaccaac aagaaggccg aggaatacaa gtctgaaaag
      301 gaagcccacg tgaccgccga tagcctgtgt gccatcaatt ggttcagacc catgagcaag
      361 agatacagca aatacgccac caccaccttc aacatgttag aactgtttaa ggagtacagc
      421 ggccacgagc ctgatgccta ttccaagaac tacctgatga gcaatatcga cagcgacaga
      481 ttcgtgtggg tggattgtag gaagttcgct aaggactttg cctatcagat ggaactgggt
      541 ttccacgagt tcaccgtgtt ggccgaaacc ctgctggcta attctatcct ggtgctgaac
      601 gagagcacca aggccaattg ggcttgggga accgtgtctg ccctgtacgg cggcggagat
      661 aaggaggaca gcacactgaa gagcaagatt ctgctggcct tcgtggacgc cctgaacaac
      721 cacgagctga aaacaaagag agaaatcttg aatcaagtgt gtgaatctct gaaataccag
      781 agctaccagg acatgtacgt ggattttaga agcgtggttg acgaaaacgg caacaagaag
      841 tctcctaacg gctctatgcc tatcgtgacc aagttcgaga cagacgacct gatcagcgac
      901 aaccaaagaa aggccatgat cagcaacttc actaagaacg ccgctgccaa ggcagctaag
      961 aaacctatcc cttacttgga ccgcctgaag gagcacatgg tgtccctgtg cgacgagtac
     1021 aatgtgtatg cctgggccgc ggccatcaca aacagcaacg ccgacgtgac cgcccggaat
     1081 accagaaacc tgacattcat cggcgaacag aacagcagac gaaaggaact gagcgtgctg
     1141 cagacaacaa ccaacgagaa ggctaaggac atcctgaaca agatcaacga caacctgatt
     1201 caggaggtgc ggtacacccc tgcccctaag cacctgggca gagatctggc caacctgttt
     1261 gatacactga aggaaaagga catcaacaac atcgagaacg aagaagagaa acagaacgtg
     1321 atcaatgact gtatcgagca gtacgtggac gattgcagaa gcctcaaccg gaaccccatc
     1381 gcagccctcc tgaagcacat ctctaggtac tacgaggatt tcagcgccaa gaatttcctg
     1441 gacggcgcca agctgaacgt gctgactgag gtggtgaacc ggcagaaggc ccaccccacc
     1501 atctggagcg agaaggctta cacctggatc agcaagttcg acaagaaccg gagacaggcc
     1561 aacagcagcc tggtcggatg ggttgtgccc cccgaggagg tgcacaagga gaaaatcgcc
     1621 ggacagcaga gcatgatgtg ggtgaccctc accctgctgg acgacggcaa gtgggtcaaa
     1681 catcacatcc ccttcagcga cagcagatac tacagcgaag tgtacgccta caaccctaat
     1741 ctgccttatc tggacggagg catcccaaga cagagcaagt tcggcaacaa accaacaacc
     1801 aacctgacag ccgagtccca ggccctcctg gctaattcta agtacaagaa agccaacaag
     1861 agcttcctgc gggctaaaga gaatgccaca cacaacgtgc gggtgtcccc taacacctct
     1921 ctgtgcatta gactgctgaa ggacagcgcc ggaaaccaga tgttcgacaa aatcggcaac
     1981 gtgctcttcg gcatgcagat caaccacaag atcaccgtgg gaaaacctaa ctacaagatc
     2041 gaggtgggcg acagattcct gggcttcgat cagaaccaga gcgagaacca cacctacgcc
     2101 gtgctgcaga gagtgtccga gagcagtcac gacacccacc actttaacgg ctgggacgtg
     2161 aaggtgctgg aaaagggcaa agtgaccagc gatgtgatcg tgcgggacga ggtctacgac
     2221 caactgtctt acgagggcgt cccctacgat agcagcaagt tcgccgagtg gcgggacaag
     2281 cgcagaagat ttgtgcttga gaacctgagc atccagctgg aagagggcaa gaccttcctg
     2341 acagagttcg acaagctgaa taaggacagc ctgtaccgct ggaacatgaa ctacctgaaa
     2401 ctgctgagaa aggccatccg ggccggaggc aaagagttcg ccaagatcgc taagacagag
     2461 atcttcgagc tggcggtgga aagattcggc cctattaacc tgggcagcct gtcccagatc
     2521 agccttaaga tgattgcctc ctttaagggc gtggtccagt cctacttctc cgtgagcggc
     2581 tgcgtggatg atgcctccaa aaaggcccat gattctatgc tgttcacatt tatgtgcgcc
     2641 gccgaagaaa agcggaccaa caagagagaa gaaaagacca acagagccgc cagctttatc
     2701 ctgcaaaaag cctacctgca tggctgcaag atgatcgtgt gcgaggacga ccttcctgtg
     2761 gccgacggca agacaggcaa agcccagaat gccgaccgga tggactggtg cgccagagcc
     2821 ctggccaaga aggtgaacga cggctgtgtt gccatgagca tctgctacag agctatccct
     2881 gcctacatga gcagccacca ggaccccttt gtgcacatgc aggataagaa aaccagcgtg
     2941 ctgcggccta gattcatgga agttaataag gatagcatca gagactacca cgtggcgggc
     3001 ctgagaagaa tgctgaacag caagagtgac gctggcacca gtgtttatta ccggcaagct
     3061 gccctgcatt tctgcgaagc cctgggcgtg agccctgaac tggtgaaaaa caagaaaacc
     3121 cacgccgccg aactgggcaa gcacatgggc agcgctatgc tgatgccctg gagaggcggt
     3181 agagtgtaca tcgccagcaa aaagctgacc tccgatgcca aatcagtgaa gtactgcggc
     3241 gaggatatgt ggcagtacca cgccgatgag atcgccgctg ttaacatcgc catgtatgag
     3301 gtgtgctgcc agaccggcgc tttcggaaag aaacagaaaa aatcggacga gctgcctgga
     3361 aaaaggccgg cggccacgaa gaaggccggc caggcaaaga agaagaagta a
//
