LOCUS       sequence-349620-        3945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector
            for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh 
            promoter and tagmCherry driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Zhang H, Kong X, Xue M, Hu J, Wang Z, Wei Y, Wang H, Zhou J, Zhang 
            W, Xu M, Shen X, Yin F, Ai Z, Huang G, Xia J, Song X, Li H, Yuan Y, 
            Li J, Zhong N, Zhang M, Zhou Y, Yang H
  TITLE     An engineered xCas12i with high activity, high specificity, and 
            broad PAM range.
  JOURNAL   Protein Cell. 2023 Jun 28;14(7):538-543. doi: 
            10.1093/procel/pwac052.
  PUBMED    37378659
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Protein Cell"; date: "2023-06-28- 28"; volume: "14"; issue: "7"; 
            pages: "538-543"
COMMENT     Sequence Label: HEpl017
FEATURES             Location/Qualifiers
     source          1..3945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..57
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3895..3942
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtctct
       61 gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc caagagggca
      121 cgggatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc
      181 gagggctgga acagagccat cggcctacac gacccaacag cccatgccga aattatggcc
      241 ctgagacaag gcggcctggt catgcagaac tacagactga ttgacgccac cctgtacgtg
      301 acattcgagc cttgcgtgat gtgcgctggc gccatgatcc actctaggat cggccgcgtg
      361 gtgtttggat ggagaaattc taaaagaggc gccgcaggct ccctgatgaa cgtgctgaac
      421 taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga tgaatgtgcc
      481 gccctgctgt gcgacttcta tcggatgcct agacaggtgt tcaatgctca gaagaaggcc
      541 cagagttcca tcaactccgg aggatctagc ggaggctcct ctggcagcga gacaccagga
      601 acaagcgagt cagcaacacc agagagcagt ggcggcagca gcggcggcag cagtatgagt
      661 tctgatgtgg tgcggcctta taacacaaag ctgctcccag ataacagaaa gcacaatatg
      721 ttcctgcaga ccttcaagcg gctgaacagc atctctctga accacttcga cctgctgatc
      781 tgcctgtacg ctgcaatcac caacaagaag gccgaggaat acaagtctga aaaggaagcc
      841 cacgtgaccg ccgatagcct gtgtgccatc aattggttca gacccatgag caagagatac
      901 agcaaatacg ccaccaccac cttcaacatg ttagaactgt ttaaggagta cagcggccac
      961 gagcctgatg cctattccaa gaactacctg atgagcaata tcgacagcga cagattcgtg
     1021 tgggtggatt gtaggaagtt cgctaaggac tttgcctatc agatggaact gggtttccac
     1081 gagttcaccg tgttggccga aaccctgctg gctaattcta tcctggtgct gaacgagagc
     1141 accaaggcca attgggcttg gggaaccgtg tctgccctgt acggcggcgg agataaggag
     1201 gacagcacac tgaagagcaa gattctgctg gccttcgtgg acgccctgaa caaccacgag
     1261 ctgaaaacaa agagagaaat cttgaatcaa gtgtgtgaat ctctgaaata ccagagctac
     1321 caggacatgt acgtggattt tagaagcgtg gttgacgaaa acggcaacaa gaagtctcct
     1381 aacggctcta tgcctatcgt gaccaagttc gagacagacg acctgatcag cgacaaccaa
     1441 agaaaggcca tgatcagcaa cttcactaag aacgccgctg ccaaggcagc taagaaacct
     1501 atcccttact tggaccgcct gaaggagcac atggtgtccc tgtgcgacga gtacaatgtg
     1561 tatgcctggg ccgcggccat cacaaacagc aacgccgacg tgaccgcccg gaataccaga
     1621 aacctgacat tcatcggcga acagaacagc agacgaaagg aactgagcgt gctgcagaca
     1681 acaaccaacg agaaggctaa ggacatcctg aacaagatca acgacaacct gattcaggag
     1741 gtgcggtaca cccctgcccc taagcacctg ggcagagatc tggccaacct gtttgataca
     1801 ctgaaggaaa aggacatcaa caacatcgag aacgaagaag agaaacagaa cgtgatcaat
     1861 gactgtatcg agcagtacgt ggacgattgc agaagcctca accggaaccc catcgcagcc
     1921 ctcctgaagc acatctctag gtactacgag gatttcagcg ccaagaattt cctggacggc
     1981 gccaagctga acgtgctgac tgaggtggtg aaccggcaga aggcccaccc caccatctgg
     2041 agcgagaagg cttacacctg gatcagcaag ttcgacaaga accggagaca ggccaacagc
     2101 agcctggtcg gatgggttgt gccccccgag gaggtgcaca aggagaaaat cgccggacag
     2161 cagagcatga tgtgggtgac cctcaccctg ctggacgacg gcaagtgggt caaacatcac
     2221 atccccttca gcgacagcag atactacagc gaagtgtacg cctacaaccc taatctgcct
     2281 tatctggacg gaggcatccc aagacagagc aagttcggca acaaaccaac aaccaacctg
     2341 acagccgagt cccaggccct cctggctaat tctaagtaca agaaagccaa caagagcttc
     2401 ctgcgggcta aagagaatgc cacacacaac gtgcgggtgt cccctaacac ctctctgtgc
     2461 attagactgc tgaaggacag cgccggaaac cagatgttcg acaaaatcgg caacgtgctc
     2521 ttcggcatgc agatcaacca caagatcacc gtgggaaaac ctaactacaa gatcgaggtg
     2581 ggcgacagat tcctgggctt cgatcagaac cagagcgaga accacaccta cgccgtgctg
     2641 cagagagtgt ccgagagcag tcacgacacc caccacttta acggctggga cgtgaaggtg
     2701 ctggaaaagg gcaaagtgac cagcgatgtg atcgtgcggg acgaggtcta cgaccaactg
     2761 tcttacgagg gcgtccccta cgatagcagc aagttcgccg agtggcggga caagcgcaga
     2821 agatttgtgc ttgagaacct gagcatccag ctggaagagg gcaagacctt cctgacagag
     2881 ttcgacaagc tgaataagga cagcctgtac cgctggaaca tgaactacct gaaactgctg
     2941 agaaaggcca tccgggccgg aggcaaagag ttcgccaaga tcgctaagac agagatcttc
     3001 gagctggcgg tggaaagatt cggccctatt aacctgggca gcctgtccca gatcagcctt
     3061 aagatgattg cctcctttaa gggcgtggtc cagtcctact tctccgtgag cggctgcgtg
     3121 gatgatgcct ccaaaaaggc ccatgattct atgctgttca catttatgtg cgccgccgaa
     3181 gaaaagcgga ccaacaagag agaagaaaag accaacagag ccgccagctt tatcctgcaa
     3241 aaagcctacc tgcatggctg caagatgatc gtgtgcgagg acgaccttcc tgtggccgac
     3301 ggcaagacag gcaaagccca gaatgccgac cggatggact ggtgcgccag agccctggcc
     3361 aagaaggtga acgacggctg tgttgccatg agcatctgct acagagctat ccctgcctac
     3421 atgagcagcc accaggaccc ctttgtgcac atgcaggata agaaaaccag cgtgctgcgg
     3481 cctagattca tggaagttaa taaggatagc atcagagact accacgtggc gggcctgaga
     3541 agaatgctga acagcaagag tgacgctggc accagtgttt attaccggca agctgccctg
     3601 catttctgcg aagccctggg cgtgagccct gaactggtga aaaacaagaa aacccacgcc
     3661 gccgaactgg gcaagcacat gggcagcgct atgctgatgc cctggagagg cggtagagtg
     3721 tacatcgcca gcaaaaagct gacctccgat gccaaatcag tgaagtactg cggcgaggat
     3781 atgtggcagt accacgccgc cgagatcgcc gctgttaaca tcgccatgta tgaggtgtgc
     3841 tgccagaccg gcgctttcgg aaagaaacag aaaaaatcgg acgagctgcc tggaaaaagg
     3901 ccggcggcca cgaagaaggc cggccaggca aagaagaaga agtaa
//
