LOCUS       sequence-349653-        9154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid to create GFP fusion proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9154)
  TITLE     ENCODE collection: CRISPR constructs
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pZBED9.1.1-gDNA
FEATURES             Location/Qualifiers
     source          1..9154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        123..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     misc_RNA        complement(505..580)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(602..850)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(660..679)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(830..850)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     rep_origin      complement(912..1500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(992..1011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1671..2462,2463..2531))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2294..2313
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2532..2636)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2704..2722
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2760..2782)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2882..2901
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2918..3373)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3055..3076)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3267..3286
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3448..3588
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3459..3588
                     /label=AAV2 ITR
     polyA_signal    3597..3804
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3793..3810
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3838..4437)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3921..3941)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4418..4437
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(4438..4491)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4507..4554)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(join(4555..8628,8629..8631,8632..8655))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4555..8655)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(8680..8700)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8707..8772)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      8772..8781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          8793..9020
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     promoter        complement(join(9021..9154,1..121))
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
ORIGIN
        1 cccccccccc cccccgcccc catcgctgca caaaataatt aaaaaataaa taaatacaaa
       61 attgggggtg gggagggggg ggagatgggg agagtgaagc agaacgtggg gctcacctcg
      121 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
      181 gtcatgtact gggcacaatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
      241 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
      301 acccattgac gtcaatggaa agtccctatt ggcgttacta ttgacgtcaa tgggcggggg
      361 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacggg tacctctaga
      421 gccatttgtc tgcagaattg gcgcacgcgc taaaaacgga ctagccttat tttaacttgc
      481 tatttctagc tctaaaacaa aaaagcaccg actcggtgcc actttttcaa gttgataacg
      541 gactagcctt attttaactt gctatttcta gctctaaaac ataccttaca tatcaatatt
      601 cggtgtttcg tcctttccac aagatatata aagccaagaa atcgaaatac tttcaagtta
      661 cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac tacccaagaa
      721 attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca gtcaaattaa
      781 ttccaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga atcatgggaa
      841 ataggccctc acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      901 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      961 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1021 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1081 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1141 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1201 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1261 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1321 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1381 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1441 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1501 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1561 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1621 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1681 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1741 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1801 atgataccgc ggcttccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1861 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1921 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1981 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2041 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2101 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2161 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2221 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2281 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2341 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2401 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2461 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2521 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2581 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2641 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2701 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     2761 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     2821 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     2881 gcggcatcag agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg
     2941 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata gaccgaaatc
     3001 ggcaaaatcc cttataaatc aaaagaatag cccgagatag agttgagtgt tgttccagtt
     3061 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3121 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg
     3181 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3241 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctaaggcg
     3301 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3361 ctacagggcg cgtactatgg ttgctttgac gtatgcggtg tgaaataccg cacagatgcg
     3421 taaggagaaa ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg
     3481 aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg
     3541 agcgagcgcg cagagaggga gtggccaact ccatcactag gggttcctgc ggccgctccc
     3601 cagcatgcct gctattctct tcccaatcct cccccttgct gtcctgcccc accccacccc
     3661 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
     3721 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
     3781 atggctggca actagaaggc acagtcgagg ctgatcagcg agctctagtt agaattctca
     3841 ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc
     3901 ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag
     3961 gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc
     4021 cagacccttg ccctggtggt cgggcgagac tccgacggtg gccaggaacc acgcgggctc
     4081 cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc
     4141 gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc
     4201 cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc
     4261 gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc
     4321 cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac
     4381 ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcattgg
     4441 gccaggattc tcctcgacgt caccgcatgt tagcagactt cctctgccct ctccactgcc
     4501 gaattccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc ttttgtcgcc
     4561 tcccagctga gacaggtcga tccgtgtctc gtacaggccg gtgatgctct ggtggatcag
     4621 ggtggcgtcc agcacctctt tggtgctggt gtacctcttc cggtcgatgg tggtgtcaaa
     4681 gtacttgaag gcggcagggg ctcccagatt ggtcagggta aacaggtgga tgatattctc
     4741 ggcctgctct ctgatgggct tatcccggtg cttgttgtag gcggacagca ctttgtccag
     4801 attagcgtcg gccaggatca ctctcttgga gaactcgctg atctgctcga tgatctcgtc
     4861 caggtagtgc ttgtgctgtt ccacaaacag ctgtttctgc tcattatcct cgggggagcc
     4921 cttcagcttc tcatagtggc tggccaggta caggaagttc acatatttgg agggcagggc
     4981 cagttcgttt cccttctgca gttcgccggc agaggccagc attctcttcc ggccgttttc
     5041 cagctcgaac agggagtact taggcagctt gatgatcagg tcctttttca cttctttgta
     5101 gcccttggct tccagaaagt cgatgggatt cttctcgaag ctgcttcttt ccatgatggt
     5161 gatccccagc agctctttca cactcttcag tttcttggac ttgccctttt ccactttggc
     5221 caccaccagc acagaatagg ccacggtggg gctgtcgaag ccgccgtact tcttagggtc
     5281 ccagtccttc tttctggcga tcagcttatc gctgttcctc ttgggcagga tagactcttt
     5341 gctgaagccg cctgtctgca cctcggtctt tttcacgata ttcacttggg gcatgctcag
     5401 cactttccgc acggtggcaa aatcccggcc cttatcccac acgatctccc cggtttcgcc
     5461 gtttgtctcg atcagaggcc gcttccggat ctcgccgttg gccagggtaa tctcggtctt
     5521 gaaaaagttc atgatgttgc tgtagaagaa gtacttggcg gtagccttgc cgatttcctg
     5581 ctcgctcttg gcgatcatct tccgcacgtc gtacaccttg tagtcgccgt acacgaactc
     5641 gctttccagc ttagggtact ttttgatcag ggcggttccc acgacggcgt tcaggtaggc
     5701 gtcgtgggcg tggtggtagt tgttgatctc gcgcactttg taaaactgga aatccttccg
     5761 gaaatcggac accagcttgg acttcagggt gatcactttc acttcccgga tcagcttgtc
     5821 attctcgtcg tacttagtgt tcatccggga gtccaggatc tgtgccacgt gctttgtgat
     5881 ctgccgggtt tccaccagct gtctcttgat gaagccggcc ttatccagtt cgctcaggcc
     5941 gcctctctcg gccttggtca gattgtcgaa ctttctctgg gtaatcagct tggcgttcag
     6001 cagctgccgc cagtagttct tcatcttctt cacgacctct tcggagggca cgttgtcgct
     6061 cttgccccgg ttcttgtcgc ttctggtcag caccttgttg tcgatggagt cgtccttcag
     6121 aaagctctga ggcacgatat ggtccacatc gtagtcggac agccggttga tgtccagttc
     6181 ctggtccacg tacatatccc gcccattctg caggtagtac aggtacagct tctcgttctg
     6241 cagctgggtg ttttccacgg ggtgttcttt caggatctgg ctgcccagct ctttgatgcc
     6301 ctcttcgatc cgcttcattc tctcgcggct gttcttctgt cccttctggg tggtctggtt
     6361 ctctctggcc atttcgatca cgatgttctc gggcttgtgc cggcccatca ctttcacgag
     6421 ctcgtccacc accttcactg tctgcaggat gcccttctta atggcggggc tgccggccag
     6481 attggcaatg tgctcgtgca ggctatcgcc ctggccggac acctgggctt tctggatgtc
     6541 ctctttaaag gtcaggctgt cgtcgtggat cagctgcatg aagtttctgt tggcgaagcc
     6601 gtcggacttc aggaaatcca ggattgtctt gccggactgc ttgtcccgga tgccgttgat
     6661 cagcttccgg ctcagcctgc cccagccggt gtatctccgc cgcttcagct gcttcatcac
     6721 tttgtcgtcg aacaggtggg cataggtttt cagccgttcc tcgatcatct ctctgtcctc
     6781 aaacagtgtc agggtcagca cgatatcttc cagaatgtcc tcgttttcct cattgtccag
     6841 gaagtccttg tccttgataa ttttcagcag atcgtggtat gtgcccaggg aggcgttgaa
     6901 ccgatcttcc acgccggaga tttccacgga gtcgaagcac tcgattttct tgaagtagtc
     6961 ctctttcagc tgcttcacgg tcactttccg gttggtcttg aacagcaggt ccacgatggc
     7021 ctttttctgc tcgccgctca ggaaggcggg ctttctcatt ccctcggtca cgtatttcac
     7081 tttggtcagc tcgttataca cggtgaagta ctcgtacagc aggctgtgct tgggcagcac
     7141 cttctcgttg ggcaggttct tatcgaagtt ggtcatccgc tcgatgaagc tctgggcgga
     7201 agcgcccttg tccaccactt cctcgaagtt ccagggggtg atggtttcct cgctctttct
     7261 ggtcatccag gcgaatctgc tgtttcccct ggccagaggg cccacgtagt aggggatgcg
     7321 gaaggtcagg atcttctcga tcttttcccg gttgtccttc aggaatgggt aaaaatcttc
     7381 ctgccgccgc agaatggcgt gcagctctcc caggtggatc tggtggggga tgctgccgtt
     7441 gtcgaaggtc cgctgcttcc gcagcaggtc ctctctgttc agcttcacga gcagttcctc
     7501 ggtgccgtcc atcttttcca ggatgggctt gatgaacttg tagaactctt cctggctggc
     7561 tccgccgtca atgtagccgg cgtagccgtt cttgctctgg tcgaagaaaa tctctttgta
     7621 cttctcaggc agctgctgcc gcacgagagc tttcagcagg gtcaggtcct ggtggtgctc
     7681 gtcgtatctc ttgatcatag aggcgctcag gggggccttg gtgatctcgg tgttcactct
     7741 caggatgtcg ctcagcagga tggcgtcgga caggttcttg gcggccagaa acaggtcggc
     7801 gtactggtcg ccgatctggg ccagcaggtt gtccaggtcg tcgtcgtagg tgtccttgct
     7861 cagctgcagt ttggcatcct cggccaggtc gaagttgctc ttgaagttgg gggtcaggcc
     7921 caggctcagg gcaatcaggt tgccgaacag gccattcttc ttctcgccgg gcagctgggc
     7981 gatcagattt tccagccgtc tgctcttgct cagtctggca gacaggatgg ccttggcgtc
     8041 cacgccgctg gcgttgatgg ggttttcctc gaacagctgg ttgtaggtct gcaccagctg
     8101 gatgaacagc ttgtccacgt cgctgttgtc ggggttcagg tcgccctcga tcaggaagtg
     8161 gccccggaac ttgatcatgt gggccagggc cagatagatc agccgcaggt cggccttgtc
     8221 ggtgctgtcc accagtttct ttctcaggtg gtagatggtg gggtacttct cgtggtaggc
     8281 cacctcgtcc acgatgttgc cgaagatggg gtgccgctcg tgcttcttat cctcttccac
     8341 caggaaggac tcttccagtc tgtggaagaa gctgtcgtcc accttggcca tctcgttgct
     8401 gaagatctct tgcagatagc agatccggtt cttccgtctg gtgtatcttc ttctggcggt
     8461 tctcttcagc cgggtggcct cggctgtttc gccgctgtcg aacagcaggg ctccgatcag
     8521 gttcttcttg atgctgtgcc ggtcggtgtt gcccagcacc ttgaatttct tgctgggcac
     8581 cttgtactcg tcggtgatca cggcccagcc cacagagttg gtgccgatgg ccaggccgat
     8641 gctgtacttc ttgtcggctg ctgggactcc gtggataccg accttccgct tcttctttgg
     8701 ggccatctta tcgtcatcgt ctttgtaatc aatatcatga tccttgtagt ctccgtcgtg
     8761 gtccttatag tccatggtgg caccggtcca acctgaaaaa aagtgatttc aggcaggtgc
     8821 tccaggtaat taaacattaa taccccacca accaaccatc ccttaaaccc ttacctcttg
     8881 ctcagctaat tacagcccgg aggagaaggg ccgtcccgcc cgctcacctg tgggagtaac
     8941 gcggtcagtc agagccgggg cgggcggcgc gaggcggcgg cggagcgggg cacggggcga
     9001 aggcagcgcg cagcgactcc cgcccgccgc gcgcttcgct ttttataggg ccgccgccgc
     9061 cgccgcctcg ccataaaagg aaactttcgg agcgcgccgc tctgattggc tgccgccgca
     9121 cctctccgcc tcgccccgcc ccgcccctcg cccc
//
