LOCUS       sequence-349699-       10766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding ETS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: ETS2_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          163..1179
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1103..1125
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1187..1206
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1295..1319
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2733..2757)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2777..2800
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2835..3384
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2979..2996)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3206..3225
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3387..3389,3390..3392,3393..4106)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3387..3389,3390..3392,3393..4103)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3432..3453)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3693..3712)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4040..4061
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4183..4771
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4236..4256)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4654..4665)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4774..4790)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4775..4791)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4980..5213
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5285..5406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5322..5341)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5376..5395
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5446..5581
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5601..5620)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5602..5620)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5643..6001)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5683..5704)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5895..5914
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6028..6132
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6133..6201,6202..6993)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6351..6370)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7164..7752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7653..7672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7927..8230
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8232..8430
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8385..8405
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8448..8628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8675..8800
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9297..9530
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9715..9759
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10057..10174
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10272..10651
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(10653..10766,1..162)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
       61 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      121 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
      181 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
      241 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
      301 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
      361 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
      421 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
      481 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
      541 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
      601 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
      661 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
      721 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
      781 gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
      841 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
      901 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
      961 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     1021 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     1081 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1141 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1201 attgtgctgt ctcatcattt tggcaaagaa ttcccctagc cgcggaatct agactgccgg
     1261 atcgaattgt cgacgaattc ggatctggcc aatcacaagt ttgtacaaaa aagcaggcac
     1321 catgaatgat ttcggaatca agaatatgga ccaggtagcc cctgtggcta acagttacag
     1381 agggacactc aagcgccagc cagcctttga cacctttgat gggtccctgt ttgctgtttt
     1441 tccttctcta aatgaagagc aaacactgca agaagtgcca acaggcttgg attccatttc
     1501 tcatgactcc gccaactgtg aattgccttt gttaaccccg tgcagcaagg ctgtgatgag
     1561 tcaagcctta aaagctacct tcagtggctt caaaaaggaa cagcggcgcc tgggcattcc
     1621 aaagaacccc tggctgtgga gtgagcaaca ggtatgccag tggcttctct gggccaccaa
     1681 tgagttcagt ctggtgaacg tgaatctgca gaggttcggc atgaatggcc agatgctgtg
     1741 taaccttggc aaggaacgct ttctggagct ggcacctgac tttgtgggtg acattctctg
     1801 ggaacatctg gagcaaatga tcaaagaaaa ccaagaaaag acagaagatc aatatgaaga
     1861 aaattcacac ctcacctccg ttcctcattg gattaacagc aatacattag gttttggcac
     1921 agagcaggcg ccctatggaa tgcagacaca gaattacccc aaaggcggcc tcctggacag
     1981 catgtgtccg gcctccacac ccagcgtact cagctctgag caggagtttc agatgttccc
     2041 caagtctcgg ctcagctccg tcagcgtcac ctactgctct gtcagtcagg acttcccagg
     2101 cagcaacttg aatttgctca ccaacaattc tgggacgccc aaagaccacg actcccctga
     2161 gaacggtgcg gacagcttcg agagctcaga ctccctcctc cagtcctgga acagccagtc
     2221 gtccttgctg gatgtgcaac gggttccttc cttcgagagc ttcgaagatg actgcagcca
     2281 gtctctctgc ctcaataagc caaccatgtc tttcaaggat tacatccaag agaggagtga
     2341 cccggtggag caaggcaaac cagttatacc tgcagctgtg ctggccggct tcacaggaag
     2401 tggacctatt cagctgtggc agtttctcct ggagctgcta tcagacaaat cctgccagtc
     2461 attcatcagc tggactggag acggatggga gtttaagctc gccgaccccg atgaggtggc
     2521 ccgccggtgg ggaaagagga aaaataagcc caagatgaac tacgagaagc tgagccgggg
     2581 cttacgctac tattacgaca agaacatcat ccacaagacg tcggggaagc gctacgtgta
     2641 ccgcttcgtg tgcgacctcc agaacttgct ggggttcacg cccgaggaac tgcacgccat
     2701 cctgggcgtc cagcccgaca cggaggactg aaacccagct ttcttgtaca aagtggtggg
     2761 aggaggtgga tctggcgact acaaggacga cgatgacaag tgactcgagc gggatcaatt
     2821 ccgccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     2881 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     2941 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     3001 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     3061 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     3121 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     3181 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     3241 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     3301 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     3361 tttcctttga aaaacacgat aataccatgg tgagcaaggg cgaggagctg ttcaccgggg
     3421 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3481 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     3541 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     3601 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     3661 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     3721 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     3781 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     3841 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     3901 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     3961 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     4021 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4081 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagagtcgac ctgcaggcat
     4141 gcaagcttca ggtagccggc taacgttatc aagcttatcg ataatcaacc tctggattac
     4201 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4261 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4321 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4381 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4441 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4501 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4561 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4621 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4681 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4741 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
     4801 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     4861 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     4921 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4981 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     5041 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5101 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5161 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     5221 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     5281 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5341 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5401 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     5461 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5521 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5581 ctagggacgt acccaattcg ccctatagtg agtcgtatta gcatgtgagc aaaaggccag
     5641 cataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     5701 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     5761 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5821 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     5881 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     5941 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     6001 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6061 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6121 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6181 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6241 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6301 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6361 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6421 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6481 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6541 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6601 gtaactcgcc ttgatcgttg ggaaccgaag ctgaatgaag ccataccaaa cgacgagcgt
     6661 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6721 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6781 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6841 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6901 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6961 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7021 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7081 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7261 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7321 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7381 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7441 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7501 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7561 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7621 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7681 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7741 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7801 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7861 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     7921 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     7981 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     8041 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     8101 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     8161 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     8221 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     8281 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     8341 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     8401 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
     8461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     8641 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     8701 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     8761 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     8821 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     8881 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     8941 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     9001 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     9061 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     9121 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     9181 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     9241 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     9301 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     9361 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     9421 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     9481 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     9541 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     9601 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     9661 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     9721 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     9781 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     9841 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     9901 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     9961 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
    10021 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
    10081 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
    10141 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
    10201 tccagtttgg ttagtaccgg gcccgctcta gccctagatt taaatcgatg tacgggccag
    10261 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    10321 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    10381 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    10441 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    10501 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    10561 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    10621 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
    10681 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
    10741 ttgtgcagcg atgggggcgg gggggg
//
