LOCUS       sequence-349701-        7281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector mediating inducible expression of 4 genes (the 
            C-prM-E-NS1) of YFV-17D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D.
  JOURNAL   Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9.
  PUBMED    34824475
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9."
COMMENT     Sequence Label: AAV-TRE-C-prM-E-NS1
FEATURES             Location/Qualifiers
     source          1..7281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    164..245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     189..208
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    257..275
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    292..310
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    328..346
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    364..382
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    400..418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    435..453
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    471..489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        501..539
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     536..560
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    4028..4504
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4227..4246)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4544..4684
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4544..4673
                     /label=AAV2 ITR
     rep_origin      4759..5214
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4846..4865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5056..5077
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5231..5250)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5350..5372
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5410..5428)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5496..5600
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5601..5669,5670..6461)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5819..5838)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6632..7220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7121..7140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgmmctsa
       61 gkscgcccgg gcgtcgggcg acctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatttg tcggcagtga aaaaaatgct
      181 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      241 aagttaacaa caacaatccc tatcagtgat agagaacgat gtcgagttta ctccctatca
      301 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
      361 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
      421 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
      481 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc
      541 gtttagtgaa ccgtcagatc gcctggagat ttcgagctcg gtaccaataa tcagccacca
      601 tgtccggtcg taaagcccag ggcaaaaccc taggcgtaaa tatggtccga cgcggagtcc
      661 gctcattgtc gaacaaaata aaacaaaaaa caaaacaaat tggaaacaga cctggacctt
      721 caagaggtgt tcaaggattt atctttttct ttttgttcaa cattttgact ggaaaaaaga
      781 tcacagccca cctaaagagg ttgtggaaaa tgctggaccc aagacaaggc ttggctgttc
      841 taaggaaagt caagagagtg gtggccagtt tgatgagagg attgtcctca aggaaacgcc
      901 gttcccatga tgttctgact gtgcaattcc taattttggg aatgctgttg atgacgggtg
      961 gagtgacctt ggtgcggaaa aacagatggt tgctcctaaa tgtgacatct gaggacctcg
     1021 ggaaaacatt ctctgtgggc acaggcaact gcacaacaaa cattttggaa gccaagtact
     1081 ggtgcccaga ctcaatggaa tacaactgtc ccaatctcag tccaagagag gagccagatg
     1141 acattgattg ctggtgctat ggggtggaaa acgttagagt cgcatatggt aagtgtgact
     1201 cagcaggcag gtctaggagg tcaagaaggg ccattgactt gcctacgcat gaaaaccatg
     1261 gtttgaagac ccggcaagaa aaatggatga ctggaagaat gggtgaaagg caactccaaa
     1321 agattgagag atggttcgtg aggaacccct tttttgcagt gacggctctg accattgcct
     1381 accttgtggg aagcaacatg acgcaacgag tcgtgattgc cctactggtc ttggctgttg
     1441 gtccggccta ctcagctcac tgcattggaa ttactgacag ggatttcatt gagggggtgc
     1501 atggaggaac ttgggtttca gctaccctgg agcaagacaa gtgtgtcact gttatggccc
     1561 ctgacaagcc ttcattggac atctcactag agacagtagc cattgataga cctgctgagg
     1621 tgaggaaagt gtgttacaat gcagttctca ctcatgtgaa gattaatgac aagtgcccca
     1681 gcactggaga ggcccaccta gccgaagaga acgaagggga caatgcgtgc aagcgcactt
     1741 attctgatag aggctggggc aatggctgtg gcctatttgg gaaagggagc attgtggcat
     1801 gcgccaaatt cacttgtgcc aaatccatga gtttgtttga ggttgatcag accaaaattc
     1861 agtatgtcat cagagcacaa ttgcatgtag gggccaagca ggaaaattgg aataccgaca
     1921 ttaagactct caagtttgat gccctgtcag gctcccagga agtcgagttc attgggtatg
     1981 gaaaagctac actggaatgc caggtgcaaa ctgcggtgga ctttggtaac agttacatcg
     2041 ctgagatgga aacagagagc tggatagtgg acagacagtg ggcccaggac ttgaccctgc
     2101 catggcagag tggaagtggc ggggtgtgga gagagatgca tcatcttgtc gaatttgaac
     2161 ctccgcatgc cgccactatc agagtactgg ccctgggaaa ccaggaaggc tccttgaaaa
     2221 cagctcttac tggcgcaatg agggttacaa aggacacaaa tgacaacaac ctttacaaac
     2281 tacatggtgg acatgtttct tgcagagtga aattgtcagc tttgacactc aaggggacat
     2341 cctacaaaat atgcactgac aaaatgtttt ttgtcaagaa cccaactgac actggccatg
     2401 gcactgttgt gatgcaggtg aaagtgtcaa aaggagcccc ctgcaggatt ccagtgatag
     2461 tagctgatga tcttacagcg gcaatcaata aaggcatttt ggttacagtt aaccccatcg
     2521 cctcaaccaa tgatgatgaa gtgctgattg aggtgaaccc accttttgga gacagctaca
     2581 ttatcgttgg gagaggagat tcacgtctca cttaccagtg gcacaaagag ggaagctcaa
     2641 taggaaagtt gttcactcag accatgaaag gcgtggaacg cctggccgtc atgggagaca
     2701 ccgcctggga tttcagctcc gctggagggt tcttcacttc ggttgggaaa ggaattcata
     2761 cggtgtttgg ctctgccttt caggggctat ttggcggctt gaactggata acaaaggtca
     2821 tcatgggggc ggtacttata tgggttggca tcaacacaag aaacatgaca atgtccatga
     2881 gcatgatctt ggtaggagtg atcatgatgt ttttgtctct aggagttggg gcggatcaag
     2941 gatgcgccat caactttggc aagagagagc tcaagtgcgg agatggtatc ttcatattta
     3001 gagactctga tgactggctg aacaagtact catactatcc agaagatcct gtgaagcttg
     3061 catcaatagt gaaagcctct tttgaagaag ggaagtgtgg cctaaattca gttgactccc
     3121 ttgagcatga gatgtggaga agcagggcag atgagatcaa tgccattttt gaggaaaacg
     3181 aggtggacat ttctgttgtc gtgcaggatc caaagaatgt ttaccagaga ggaactcatc
     3241 cattttccag aattcgggat ggtctgcagt atggttggaa gacttggggt aagaaccttg
     3301 tgttctcccc agggaggaag aatggaagct tcatcataga tggaaagtcc aggaaagaat
     3361 gcccgttttc aaaccgggtc tggaattctt tccagataga ggagtttggg acgggagtgt
     3421 tcaccacacg cgtgtacatg gacgcagtct ttgaatacac catagactgc gatggatcta
     3481 tcttgggtgc agcggtgaac ggaaaaaaga gtgcccatgg ctctccaaca ttttggatgg
     3541 gaagtcatga agtaaatggg acatggatga tccacacctt ggaggcatta gattacaagg
     3601 agtgtgagtg gccactgaca catacgattg gaacatcagt tgaagagagt gaaatgttca
     3661 tgccgagatc aatcggaggc ccagttagct ctcacaatca tatccctgga tacaaggttc
     3721 agacgaacgg accttggatg caggtaccac tagaagtgaa gagagaagct tgcccaggga
     3781 ctagcgtgat cattgatggc aactgtgatg gacggggaaa atcaaccaga tccaccacgg
     3841 atagcgggaa agttattcct gaatggtgtt gccgctcctg cacaatgccg cctgtgagct
     3901 tccatggtag tgatgggtgt tggtatccca tggaaattag gccaaggaaa acgcatgaaa
     3961 gccatctggt gcgctcctgg gttacagctt gaataaatcg ataccgagcg ctgctcgaga
     4021 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     4081 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     4141 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     4201 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     4261 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     4321 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     4381 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     4441 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     4501 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     4561 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     4621 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     4681 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4741 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4801 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     4861 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4921 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4981 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5041 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     5101 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     5161 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     5221 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     5281 cacccgctga cgcgccctga ccggcttgtc tgctcccggc atccgcttac agacaagctg
     5341 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5401 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5461 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5521 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5581 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5641 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5701 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5761 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5821 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5881 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5941 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6001 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6061 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6121 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6181 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6241 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6301 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6361 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6421 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6481 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6541 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6601 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6661 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6721 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6781 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6841 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6901 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6961 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7021 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7081 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7141 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7201 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7261 gctggccttt tgctcacatg t
//
