LOCUS       sequence-349709-        4996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector mediating inducible expression of the NS1 gene of 
            YFV-17D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D.
  JOURNAL   Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9.
  PUBMED    34824475
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9."
COMMENT     Sequence Label: AAV-TRE-NS1NF
FEATURES             Location/Qualifiers
     source          1..4996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    138..219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     163..182
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    231..249
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    266..284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    302..320
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    338..356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    374..392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    409..427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    445..463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        475..513
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     510..534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    1743..2219
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1942..1961)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2259..2399
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2259..2388
                     /label=AAV2 ITR
     rep_origin      2474..2929
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2561..2580)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2771..2792
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2946..2965)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3065..3087
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3125..3143)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3211..3315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3316..3384,3385..4176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3534..3553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4347..4935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4836..4855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgacctca
       61 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc
      121 cgcacgcgta tttgtcggca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
      181 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa tccctatcag
      241 tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
      301 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
      361 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg
      421 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg
      481 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agatttcgag ctcggtacca ataatcagcc accatgaaca tgacaatgtc catgagcatg
      601 atcttggtag gagtgatcat gatgtttttg tctctaggag ttggggcgga tcaaggatgc
      661 gccatcaact ttggcaagag agagctcaag tgcggagatg gtatcttcat atttagagac
      721 tctgatgact ggctgaacaa gtactcatac tatccagaag atcctgtgaa gcttgcatca
      781 atagtgaaag cctcttttga agaagggaag tgtggcctaa attcagttga ctcccttgag
      841 catgagatgt ggagaagcag ggcagatgag atcaatgcca tttttgagga aaacgaggtg
      901 gacatttctg ttgtcgtgca ggatccaaag aatgtttacc agagaggaac tcatccattt
      961 tccagaattc gggatggtct gcagtatggt tggaagactt ggggtaagaa ccttgtgttc
     1021 tccccaggga ggaagaatgg aagcttcatc atagatggaa agtccaggaa agaatgcccg
     1081 ttttcaaacc gggtctggaa ttctttccag atagaggagt ttgggacggg agtgttcacc
     1141 acacgcgtgt acatggacgc agtctttgaa tacaccatag actgcgatgg atctatcttg
     1201 ggtgcagcgg tgaacggaaa aaagagtgcc catggctctc caacattttg gatgggaagt
     1261 catgaagtaa atgggacatg gatgatccac accttggagg cattagatta caaggagtgt
     1321 gagtggccac tgacacatac gattggaaca tcagttgaag agagtgaaat gttcatgccg
     1381 agatcaatcg gaggcccagt tagctctcac aatcatatcc ctggatacaa ggttcagacg
     1441 aacggacctt ggatgcaggt accactagaa gtgaagagag aagcttgccc agggactagc
     1501 gtgatcattg atggcaactg tgatggacgg ggaaaatcaa ccagatccac cacggatagc
     1561 gggaaagtta ttcctgaatg gtgttgccgc tcctgcacaa tgccgcctgt gagcttccat
     1621 ggtagtgatg ggtgttggta tcccatggaa attaggccaa ggaaaacgca tgaaagccat
     1681 ctggtgcgct cctgggttac agcttgaata aatcgatacc gagcgctgct cgagagatct
     1741 acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
     1801 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
     1861 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
     1921 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
     1981 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
     2041 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
     2101 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
     2161 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
     2221 tgattttgta ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     2281 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     2341 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     2461 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2521 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     2581 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2641 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     2701 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2761 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     2821 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     2881 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     2941 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3001 gctgacgcgc cctgaccggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3061 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     3121 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     3181 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3241 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3301 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3361 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3421 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3481 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3541 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3601 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3661 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3721 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3781 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3841 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3901 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3961 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4021 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4081 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4141 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4201 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4261 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4321 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4381 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4441 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4501 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4561 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4621 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4681 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4741 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4801 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4861 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4921 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4981 ccttttgctc acatgt
//
